Centromeric repetitive DNA sequences in the genus Brassica

Harrison, G. E.; Heslop-Harrison, J. S.

doi:10.1007/bf00222197

Cited by 144 publications

(82 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This close relationship is also indicated from FISH experiments because only a few probes appear to be species specific. Some sequences used as probes, such as the ''centromeric retrotransposon of Brassica'' and a tandem repeat (TR805), hybridized to chromosomes of all three species (Lim et al 2007) whereas others, for example, the tandem repeat sequences pBcKB4 (CentBr1), pBoKB1, and CentBr2, hybridized to several chromosomes of both the A and C genomes but not to the B genome (Harrison and HeslopHarrison 1995;Lim et al 2005Lim et al , 2007.…”

Section: Discussionmentioning

confidence: 99%

A and C Genome Distinction and Chromosome Identification inBrassica napusby Sequential Fluorescencein SituHybridization and Genomicin SituHybridization

et al. 2008

View full text Add to dashboard Cite

The two genomes (A and C) of the allopolyploid Brassica napus have been clearly distinguished using genomic in situ hybridization (GISH) despite the fact that the two extant diploids, B. rapa (A, n ¼ 10) and B. oleracea (C, n ¼ 9), representing the progenitor genomes, are closely related. Using DNA from B. oleracea as the probe, with B. rapa DNA and the intergenic spacer of the B. oleracea 45S rDNA as the block, hybridization occurred on 9 of the 19 chromosome pairs along the majority of their length. The pattern of hybridization confirms that the two genomes have remained distinct in B. napus line DH12075, with no significant genome homogenization and no large-scale translocations between the genomes. Fluorescence in situ hybridization (FISH)-with 45S rDNA and a BAC that hybridizes to the pericentromeric heterochromatin of several chromosomes-followed by GISH allowed identification of six chromosomes and also three chromosome groups. Our procedure was used on the B. napus cultivar Westar, which has an interstitial reciprocal translocation. Two translocated segments were detected in pollen mother cells at the pachytene stage of meiosis. Using B. oleracea chromosome-specific BACs as FISH probes followed by GISH, the chromosomes involved were confirmed to be A7 and C6.

show abstract

Section: Discussionmentioning

confidence: 99%

A and C Genome Distinction and Chromosome Identification inBrassica napusby Sequential Fluorescencein SituHybridization and Genomicin SituHybridization

et al. 2008

View full text Add to dashboard Cite

show abstract

“…OB420) (Maluszynska & HeslopHarrison, 1993) and B. oleracea cv. BrauncschWeiger (Snowdon et al, 1997) for 45S rDNA and cultivar Dwarf green curled for pBcKB4 (Harrison & Heslop-Harrison, 1995).…”

Section: Discussionmentioning

confidence: 99%

“…(III) Plasmids pBoKB1 derived from B. oleracea and pBcKB4 derived from B. campestris containing repetitive DNA clones from the pericentromeric heterochromatin (Harrison & Heslop-Harrison, 1995).…”

Section: Probesmentioning

confidence: 99%

“…Earlier studies have established the numbers, but not the positions of loci, for 18S-5.8S-25S (45S) and 5S rDNA, and also analysed a repetitive DNA present in pericentromeric heterochromatin of Brassica mitotic chromosomes (Maluszynska & Heslop-Harrison, 1993;Harrison & Heslop-Harrison, 1995;Brandes & Heslop-Harrison, 1996). In this paper we determine, for the first time, the chromosomal positions of 45S rDNA and 5S rDNA sequences for Brassica oleracea var.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. alboglabra by fluorescence in situ hybridization

et al. 1998

View full text Add to dashboard Cite

As part of a programme to integrate the genetic and physical chromosome maps of Brassica oleracea we have carried out fluorescence in situ hybridization (FISH) to mitotic and meiotic chromosomes of B. oleracea var. alboglabra, using repeat sequence DNA probes. We have confirmed that there are three 18S-5.8S-25S rDNA sites in the haploid genome and accurately determined their locations for the first time; two occur subtelomerically on the short arms of the two satellited acrocentric chromosomes (chromosomes 4 and 7), whereas the third site is adjacent to the centromere on the short arm of a large submetacentric chromosome (chromosome 2). 5S rDNA sequences are located on the long arm of this same chromosome, with closely adjacent major and minor loci. A highly repeated sequence (pBcKB4) colocalizes with the pericentromeric heterochromatin on all chromosomes, but six chromosomes exhibit very strong signals whereas the remaining three show only weak signals. The construction of a partial karyotype for B. oleracea var. alboglabra, based on several major cytogenetical landmarks provided by FISH localization of repetitive DNA sequences, provides a valuable framework for the future physical mapping of nonrepetitive sequences.

show abstract

“…Chromosome-specific satellite sequence families have been reported in both animal and plant species (e.g., Warburton et al, 1993;Choo et al, 1994;Harrison and Heslop-Harrison, 1995;Gindullis et al, 2001). Like T .…”

mentioning

confidence: 99%

Chromosome-specific satellite sequences in Turritis glabra

Kawabe

Nasuda

2006

Genes Genet. Syst.

View full text Add to dashboard Cite

Two novel repetitive sequence families were isolated from Turritis glabra (2n = 2x = 12). These two repeat families are similar to those of centromeric repeats in Arabidopsis thaliana, are co-localized on one chromosome pair, and differ by about 20% from each other. Phylogenetic analysis revealed that the two repeat families of T . glabra are more similar to each other than to the centromeric repeat families of other Arabidopsis and related species. The relationships of satellite sequences reflected the species phylogeny, indicating that the replacement of satellite sequences has occurred in each species lineage independently, and shared variants could not have existed for a long time between species.

show abstract

Centromeric repetitive DNA sequences in the genus Brassica

Cited by 144 publications

References 32 publications

A and C Genome Distinction and Chromosome Identification inBrassica napusby Sequential Fluorescencein SituHybridization and Genomicin SituHybridization

A and C Genome Distinction and Chromosome Identification inBrassica napusby Sequential Fluorescencein SituHybridization and Genomicin SituHybridization

Physical mapping of DNA repetitive sequences to mitotic and meiotic chromosomes of Brassica oleracea var. alboglabra by fluorescence in situ hybridization

Chromosome-specific satellite sequences in Turritis glabra

Contact Info

Product

Resources

About