Centromeric DNA of Kluyveromyces lactis

Random sequencing of the Kluyveromyces lactis genome allowed the identification of 2235^2601 open reading frames (ORFs) homologous to S. cerevisiae ORFs, 51 ORFs which were homologous to genes from other species, 64 tRNAs, the complete rDNA repeat, and a few Ty1-and Ty2-like sequences. In addition, the complete sequence of plasmid pKD1 and a large coverage of the mitochondrial genome were obtained. The global distribution into general functional categories found in Saccharomyces cerevisiae and as defined by MIPS is well conserved in K. lactis. However, detailed examination of certain subcategories revealed a small excess of genes involved in amino acid metabolism in K. lactis. The sequences are deposited at EMBL under the accession numbers AL424881Â L430960. ß 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

show abstract

“…Five out of the six centromeres of K. lactis are known [21]. We compared their sequence to our data and identi¢ed only part of KlCEN3 in one RST.…”

Section: Centromeresmentioning

confidence: 94%

“…Five of the six centromeres have been characterized [21]. A genetic map of the K. lactis genome, describing 75 loci, has been published in 1995 [22] but an updated survey of databases reveals that 150 chromosomal genes of K. lactis have been cloned and entirely sequenced.…”

Section: Introductionmentioning

confidence: 99%

Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis

et al. 2000

View full text Add to dashboard Cite

show abstract

“…MA Ta/MA Tot,TRP1/trp l , Heus et al (1993 a) the suitable separation of chromosomes IV and V (Heus et al 1990). The chromosomes were separated using a contour-clamped homogenous electric field (CHEF) apparatus (Bio-Rad).…”

Section: This Study Gg1901mentioning

confidence: 99%

Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable, despite low overall homology

Mulder¹,

Winkler

Scholten³

et al. 1994

Curr Genet

Self Cite

View full text Add to dashboard Cite

General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Download date: 10 May 2018Curr Genet (1994)26:198-207 Current Genetics The KICPF1 gene, coding for the centromere and promoter factor CPF1 from Kluyveromyces lactis, has been cloned by functional complementation of the methionine auxotrophic phenotype of a Saccharomyces cerevisiae mutant lacking ScCPF1. The amino-acid sequences of both CPF1 proteins show a relatively-low overall identity (31%), but a highly-homologous C-terminal domain (86%). This region constitutes the DNA-binding domain with basic-helix-loop-helix and leucine-zipper motifs, features common to the myc-related transcription factor family. The N-terminal two-thirds of the CPF1 proteins show no significant similarity, although the presence of acidic regions is a shared feature. In K1CPF1, the acidic region is a prominent stretch of approximately 40 consecutive aspartate and glutamate residues, suggesting that this part might be involved in transcriptional activation. In-vitro mobility-shift experiments were used to establish that both CPF1 proteins bind to the consensus binding site RTCACRTG (CDEI element). In contrast to S. cerevisiae, CPF1 genedisruption is lethal in K. lactis. The homologous CPF1 genes were transformed to both S. cerevisiae and K. lactis cpfl-null strains. Indistinguishable phenotypes were observed, indicating that, not withstanding the long nonconserved N-terminal region, the proteins are sufficiently homologous to overcome the phenotypes associated with cpfl gene-disruption.

show abstract

“…The centromere DNA (CEN DNA) consists of 125 bp in S. cerevisiae [1] and 250 bp in Kluyveromyces lactis [2]. In contrast, the centromere DNA of mammalian chromosomes is estimated to contain at least 1 Mb and functional centromere DNA isolated from Drosophila melanogaster [3] or Schizosaccharomyces pombe [4] consists of at least 220 kb or 40 kb respectively.…”

Section: Cen Dna Binding Proteins I Introductionmentioning

confidence: 99%

The Saccharomyces cerevisiae kinetochore

Lechner¹,

Ortiz²

1996

FEBS Letters

View full text Add to dashboard Cite

Accurate chromosome segregation is dependent on a specialized chromosomal structure, the kinetochore/centromere. The only essential constituent of the S. cerevisiae kinetochore established today is CBF3, a multisubunit complex that binds to S. cerevisiae centromere DNA. Therefore CBF3 and its four components, Cbf3a, Cbf3b, Cbf3c and Cbf3d, will form the centerpiece of this review. In addition, we will describe proteins that are putatively involved in kinetochore function specifically in the context with CBF3 interaction. Furthermore, we discuss the role of the S. cerevisiae kinetochores in a putative cell cycle checkpoint control and in microtubule attachment. Key words:Centromere; Kinetochore; CBF3; Saccharomyces cerevisiae Recent reviews that describe the S. cerevisiae kinetochore are available [1,5,6]. This review focuses on the protein components of the S. cerevisiae kinetochore. First we describe proteins that physically interact with the CEN DNA and we emphasize the analysis of CBF3 (_centromere DNA binding factor), a key component of the S. cerevisiae kinetochore. Second we describe proteins that are putatively involved in kinetochore function (as structural components or regulators) as deduced from genetic interactions with CEN DNA or CEN DNA binding proteins. Finally we discuss the role of the S. cerevisiae kinetochore as a putative cell cycle checkpoint and in microtubule interaction. CEN DNA binding proteins

show abstract

Centromeric DNA of Kluyveromyces lactis

Cited by 67 publications

References 39 publications

Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis

Genomic Exploration of the Hemiascomycetous Yeasts: 11. Kluyveromyces lactis

Centromere promoter factors (CPF1) of the yeasts Saccharomyces cerevisiae and Kluyveromyces lactis are functionally exchangeable, despite low overall homology

The Saccharomyces cerevisiae kinetochore

Contact Info

Product

Resources

About