Centromere-linkage in the turbot (Scophthalmus maximus) through half-tetrad analysis in diploid meiogynogenetics

Martı́nez, Paulino; Hermida, Miguel; Pardo, Belén G.; Fernández, Carlos; Castro, Jaime; Cal, Rosa; Álvarez‐Dios, José Antonio; Bouza, Carmen

doi:10.1016/j.aquaculture.2008.05.011

Cited by 46 publications

(59 citation statements)

References 41 publications

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“…An accurate centromere position could aid both in interpreting recombination frequencies in terms of physical distances in its vicinity and in explaining previous sex ratios observed in turbot gynogenetic and triploid progenies (Cal et al 2006a,b). In Figure 3, the joint segregation analysis for both markers and the two alternative centromere locations-I (Martínez et al 2008) and II (present data) in Figure 3-is presented. Joint segregation evidenced the necessity of 25 double recombinants to explain the data under hypothesis I, while only 1 double recombinant would be necessary under hypothesis II.…”

Section: Resultsmentioning

confidence: 99%

“…A more accurate location of centromere at LG5 was determined by analyzing a large sample (96 individuals) in the reference diploid gynogenetic family with the two closest informative markers to the centromere, Sma-USC270 and Sma-USC65 (Martínez et al 2008). An accurate centromere position could aid both in interpreting recombination frequencies in terms of physical distances in its vicinity and in explaining previous sex ratios observed in turbot gynogenetic and triploid progenies (Cal et al 2006a,b).…”

Section: Resultsmentioning

confidence: 99%

“…The position of the centromere at LG5 was reanalyzed using previous data and new information obtained after genotyping 96 individuals of the reference diploid gynogenetic family (Martínez et al 2008) with the closest informative centromere markers. Complete interference was used for estimating locus-centromere distances, and joint segregation analysis was applied to order the group of closely linked markers and the centromere (Thorgaard et al 1983).…”

Section: Statistical Proceduresmentioning

confidence: 99%

“…Genetic map refinement: The turbot genetic map (Bouza et al 2007Martínez et al 2008) was reanalyzed at LG5, where the main sex-associated QTL was located (see results). Previous mapping data in the reference haploid (HF) and diploid (DF ¼ Qfam1) families (Bouza et al 2007) were revised, and missing data were supplied.…”

Section: Statistical Proceduresmentioning

confidence: 99%

“…In this study, we used the turbot genetic map (Bouza et al 2007Martínez et al 2008) to look for sexassociated QTL in this species. The identification of a major QTL in a specific linkage group (LG) in the five families analyzed prompted us to refine the genetic map at this LG and to perform a comparative genomics approach against model fish species for a precise location and characterization of the putative SD region.…”

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confidence: 99%

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Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

et al. 2009

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Sex determination in fish is a labile character in evolutionary terms. The sex-determining (SD) master gene can differ even between closely related fish species. This group is an interesting model for studying the evolution of the SD region and the gonadal differentiation pathway. The turbot (Scophthalmus maximus) is a flatfish of great commercial value, where a strong sexual dimorphism exists for growth rate. Following a QTL and marker association approach in five families and a natural population, we identified the main SD region of turbot at the proximal end of linkage group (LG) 5, close to the SmaUSC-E30 marker. The refined map of this region suggested that this marker would be 2.6 cM and 1.4 Mb from the putative SD gene. This region appeared mostly undifferentiated between males and females, and no relevant recombination frequency differences were detected between sexes. Comparative genomics of LG5 marker sequences against five model species showed no similarity of this chromosome to the sex chromosomes of medaka, stickleback, and fugu, but suggested a similarity to a sex-associated QTL from Oreochromis spp. The segregation analysis of the closest markers to the SD region demonstrated a ZW/ZZ model of sex determination in turbot. A small proportion of families did not fit perfectly with this model, which suggests that other minor genetic and/or environmental factors are involved in sex determination in this species.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Statistical Proceduresmentioning

confidence: 99%

Section: Statistical Proceduresmentioning

confidence: 99%

mentioning

confidence: 99%

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Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

et al. 2009

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Reproduction and Sex Control in Turbot

Taboada

Robledo

Bouza

et al. 2018

Sex Control in Aquaculture

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Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones

et al. 2014

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Bacterial artificial chromosomes (BAC) have been widely used for fluorescence in situ hybridization (FISH) mapping of chromosome landmarks in different organisms, including a few in teleosts. In this study, we used BAC-FISH to consolidate the previous genetic and cytogenetic maps of the turbot (Scophthalmus maximus), a commercially important pleuronectiform. The maps consisted of 24 linkage groups (LGs) but only 22 chromosomes. All turbot LGs were assigned to specific chromosomes using BAC probes obtained from a turbot 5× genomic BAC library. It consisted of 46,080 clones with inserts of at least 100 kb and <5 % empty vectors. These BAC probes contained gene-derived or anonymous markers, most of them linked to quantitative trait loci (QTL) related to productive traits. BAC clones were mapped by FISH to unique marker-specific chromosomal positions, which showed a notable concordance with previous genetic mapping data. The two metacentric pairs were cytogenetically assigned to LG2 and LG16, and the nucleolar organizer region (NOR)-bearing pair was assigned to LG15. Double-color FISH assays enabled the consolidation of the turbot genetic map into 22 linkage groups by merging LG8 with LG18 and LG21 with LG24. In this work, a first-generation probe panel of BAC clones anchored to the turbot linkage and cytogenetical map was developed. It is a useful tool for chromosome traceability in turbot, but also relevant in the context of pleuronectiform karyotypes, which often show small hardly identifiable chromosomes. This panel will also be valuable for further integrative genomics of turbot within Pleuronectiformes and teleosts, especially for fine QTL mapping for aquaculture traits, comparative genomics, and whole-genome assembly.

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Centromere-linkage in the turbot (Scophthalmus maximus) through half-tetrad analysis in diploid meiogynogenetics

Cited by 46 publications

References 41 publications

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

Identification of the Major Sex-Determining Region of Turbot (Scophthalmus maximus)

Reproduction and Sex Control in Turbot

Consolidation of the genetic and cytogenetic maps of turbot (Scophthalmus maximus) using FISH with BAC clones

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