Centrobin

Abstract

In mammalian cells, the centrosome consists of a pair of centrioles and amorphous pericentriolar material. The pair of centrioles, which are the core components of the centrosome, duplicate once per cell cycle. Centrosomes play a pivotal role in orchestrating the formation of the bipolar spindle during mitosis. Recent studies have linked centrosomal activity on centrioles or centriole-associated structures to cytokinesis and cell cycle progression through G1 into the S phase. In this study, we have identified centrobin as a centriole-associated protein that asymmetrically localizes to the daughter centriole. The silencing of centrobin expression by small interfering RNA inhibited centriole duplication and resulted in centrosomes with one or no centriole, demonstrating that centrobin is required for centriole duplication. Furthermore, inhibition of centriole duplication by centrobin depletion led to impaired cytokinesis.

Centrosomal nucleolin is required for microtubule network organization

“…4M left, black bar). In agreement with previous publication, 36 centrobin was found associated with both centrioles in more than 50% of the cells (Fig. 4M, left, gray bar).…”

Centrobin/NIP2 Is a Microtubule Stabilizer Whose Activity Is Enhanced by PLK1 Phosphorylation during Mitosis

“…Centrobin is expressed abundantly in tissues with high proliferation activities (14); however, it was also detected in the centrosomes of resting cells, suggesting that centrobin is a core centrosome protein required for basic functions of the centrosome (15). Furthermore, centrobin is involved in the microtubule organizing activity of the cell (16).…”

“…Centrobin/NIP2 was initially identified as a centrosome protein required for centriole duplication (14). Centrobin is expressed abundantly in tissues with high proliferation activities (14); however, it was also detected in the centrosomes of resting cells, suggesting that centrobin is a core centrosome protein required for basic functions of the centrosome (15).…”

C-NAP1 and rootletin restrain DNA damage-induced centriole splitting and facilitate ciliogenesis

“…Primary cilia play a sensory role and regulate cell signaling γ-tubulin, as our reference marker in these experiments, because it consistently colocalized with the centriole marker, centrin ( Table 1), indicating that no PCM fragmentation occurred after IR, and because it gave a robust signal with no DNA damageinduced granules or background, 30 allowing unambiguous identification of centrioles. Isolated centrioles were positive for a number of markers that indicated a normal centriole composition and an associated PCM: centrin, 31 centrobin, 32 C-NAP1, 15 γ-tubulin, glutamylated tubulin, 33 NEDD1, 34,35 pericentrin 36 and rootletin, 14 which all localize to centrosomes in untreated cells ( Fig. 1A and Table 1; Fig.…”

“…Given that CEP170 and ninein can localize to proximal centriole ends, the absence of kizuna and cenexin from the split centrioles suggests that the daughter centriole tends to become isolated after irradiation. To confirm these analyses with centriole-specific markers, we quantitated cenexin and the daughter-associated protein, centrobin, 32 at the split centrioles along with centrin2 and centrin3. In 50 cells examined per experiment, the split centriole showed cenexin staining in 12% of cells and centrobin in 84% (Fig.…”