Centrobin-mediated Regulation of the Centrosomal Protein 4.1-associated Protein (CPAP) Level Limits Centriole Length during Elongation Stage

Gudi, Radhika; Haycraft, Courtney J.; Bell, P. Darwin; Li, Zihai; Vasu, Chenthamarakshan

doi:10.1074/jbc.m114.603423

Cited by 29 publications

(38 citation statements)

References 62 publications

(116 reference statements)

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“…1). The proteins that comprise these appendages are enriched specifically at the mother centriole, whereas the daughter centriole possesses its own specific molecular components (e.g., centrobin-a protein required to ensure the length of the newly formed centrioles; Zou et al 2005;Gudi et al 2015). The current proposed role for distal appendages is in docking the centrosome to the plasma membrane during the formation of cilia (Tanos et al 2013), whereas the subdistal appendages are proposed to act in MTanchoring in interphase cells (Delgehyr et al 2005).…”

Section: Introduction: Centrosomes-small But Not Simplementioning

confidence: 99%

The Centrosome, a Multitalented Renaissance Organelle

Vertii

Hehnly

Doxsey

2016

Cold Spring Harb Perspect Biol

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The centrosome acts as a microtubule-organizing center (MTOC) from the G to G phases of the cell cycle; it can mature into a spindle pole during mitosis and/or transition into a cilium by elongating microtubules (MTs) from the basal body on cell differentiation or cell cycle arrest. New studies hint that the centrosome functions in more than MT organization. For instance, it has recently been shown that a specific substructure of the centrosome-the mother centriole appendages-are required for the recycling of endosomes back to the plasma membrane. This alone could have important implications for a renaissance in our understanding of the development of primary cilia, endosome recycling, and the immune response. Here, we review newly identified roles for the centrosome in directing membrane traffic, the immunological synapse, and the stress response.

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Section: Introduction: Centrosomes-small But Not Simplementioning

confidence: 99%

The Centrosome, a Multitalented Renaissance Organelle

Vertii

Hehnly

Doxsey

2016

Cold Spring Harb Perspect Biol

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“…The role of CPAP in centriole biogenesis and centrosome function is well established (38,41,(48)(49)(50)(51)(52). During our studies to determine the interaction between CPAP and other centriolar proteins, we observed that both HEK293T and HeLa cells transfected with GFP-CPAP showed spontaneous formation of punctate structures.…”

Section: Cpap Overexpression Causes the Formation Of "Mvb-like" Strucmentioning

confidence: 73%

Centrosomal P4.1-associated protein (CPAP) positively regulates endocytic vesicular transport and lysosome targeting of EGFR

Gudi

Palanisamy

Vasu

2020

Preprint

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Centrosomal P4.1-associated protein (CPAP) plays a critical role in restricting the centriole length in human cells. Here, we report a novel positive regulatory role for CPAP in endosome maturation during internalized-cell surface receptor trafficking. We found that CPAP is required for targeting ligand-bound cell surface receptor to the lysosome for degradation. While routing of ligand-engaged receptor, which is targeted for degradation or recycling, into early endosomes is not impacted by CPAP-deficiency, sequential transfer of the receptor to late endosome is severely diminished in CPAP-depleted cells. Importantly, activation and recruitment of Rab7, but not Rab5, is compromised upon CPAP depletion indicating its positive regulatory role in early to late endosome transition. Overall, this regulatory role of CPAP in endosome maturation provides new insights in understanding the cellular functions of CPAP, which is well recognized to be critical for centriole duplication and ciliogenesis.

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“…Recently, it was reported that centrobin induces centriole elongation through regulating the CPAP level which is also a crucial factor for centriole growth. 15,16 It remains to be investigated what is the biological significance of cytoplasmic centrobin. It is known that both NEK2 and PLK1 phosphorylate centrobin at specific sites but result in opposite outcomes.…”

Section: Discussionmentioning

confidence: 99%

The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm

Shin

Kaang

et al. 2015

Cell Cycle

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Centrobin resides in daughter centriole and play a critical role in centriole duplication. Nucleation and stabilization of microtubules are known biological activities of centrobin. Here, we report a specific localization of centrobin outside the centrosome. Centrobin was associated with the stable microtubules. In hippocampal cells, centrobin formed cytoplasmic dots in addition to the localization at both centrosomes with the mother and daughter centrioles. Such specific localization pattern suggests that cytoplasmic centrobin is not just a reserved pool for centrosomal localization but also has a specific role in the cytoplasm. In fact, centrobin enhanced microtubule formation outside as well as inside the centrosome. These results propose specific roles of the cytoplasmic centrobin for noncentrosomal microtubule formation in specific cell types and during the cell cycle.

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Centrobin-mediated Regulation of the Centrosomal Protein 4.1-associated Protein (CPAP) Level Limits Centriole Length during Elongation Stage

Cited by 29 publications

References 62 publications

The Centrosome, a Multitalented Renaissance Organelle

The Centrosome, a Multitalented Renaissance Organelle

Centrosomal P4.1-associated protein (CPAP) positively regulates endocytic vesicular transport and lysosome targeting of EGFR

The microtubule nucleation activity of centrobin in both the centrosome and cytoplasm

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