Centripetal wall formation in roots ofVicia faba after caffeine treatment

Röper, W.; Röper, Sybille

doi:10.1007/bf01276284

Cited by 22 publications

(7 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Assaad et al (1996) described comparable cell wall defects in keule embryos. Similar abnormalities were induced by incubating different plant seedlings with drugs, such as caffeine in bean (Röper and Röper 1977), or treating with herbicides, such as dichlobenil in onion (Vaughn et al 1996). Both treatments disrupted cell plate formation and led to extensions of the parental wall, referred to as centripetal wall formation by Röper and Röper (1977).…”

Section: Discussionmentioning

confidence: 85%

“…Similar abnormalities were induced by incubating different plant seedlings with drugs, such as caffeine in bean (Röper and Röper 1977), or treating with herbicides, such as dichlobenil in onion (Vaughn et al 1996). Both treatments disrupted cell plate formation and led to extensions of the parental wall, referred to as centripetal wall formation by Röper and Röper (1977). Abnormal embryos with variably enlarged cells, incomplete cell walls and multiple nuclei were also seen in A. thaliana mutants in which HINKEL, a kinesin-related protein, was not functional (Strompen et al 2002).…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Unique cell wall abnormalities in the putative phosphoinositide phosphatase mutant AtSAC9

Vollmer

Youssef

DeWald

2011

Planta

View full text Add to dashboard Cite

SAC9 is a putative phosphoinositide phosphatase in Arabidopsis thaliana involved in phosphoinositide signaling. sac9-1 plants have a constitutively stressed phenotype with shorter roots which notably accumulate phosphatidylinositol 4,5-bisphosphate and its hydrolysis product inositol trisphosphate. We investigated the primary roots of sac9-1 seedlings at the cytological and ultrastructural level to determine the structural basis for this altered growth. Despite the normal appearance of organelles and cytoplasmic elements, our studies reveal extreme abnormalities of cell wall and membrane structures in sac9-1 primary root cells, regardless of cell type, position within the meristematic area, and plane of section. Cell wall material was deposited locally and in a range of abnormal shapes, sometimes completely fragmenting the cell. Simple protuberances, broad flanges, diffuse patches, elaborate folds, irregular loops and other complex three-dimensional structures were found to extend randomly from the pre-existing cell wall. Abundant vesicles and excessive membrane material were associated with these irregular wall structures. We argue that a perturbed phosphoinositide metabolism most likely induces these observed abnormalities and hypothesize that a disorganized cytoskeleton and excessive membrane trafficking mediate the cell wall defects.

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 87%

Unique cell wall abnormalities in the putative phosphoinositide phosphatase mutant AtSAC9

Vollmer

Youssef

DeWald

2011

Planta

View full text Add to dashboard Cite

show abstract

“…This circumferential microtubule array formed after exposure to Benlate is comparable to those found with caffeine treatment, as studied by a number of other groups. It is well known that, without detectably interfering with phragmoplast microtubules during cytokinesis, caffeine prevents the completion of new cell wall formation (Pickett-Heaps, 1969;Roper and Roper, 1977;Pareyre, Lasselain, and Deysson, 1979;Bonsignore and Hepler, 1985;Galatis and Apostolakos, 1991). Among II 6.0 (0.82) 11.2 (0.96)g 3.0 (-) 19.0 (4.97) 10 giliter 6 1.7 (0.24)" 3.0 (0.71)" 0.4 (0.54)" 3.0 (1.65)" II 2.3 (0.52)" 4.3 (0.98)" 1.5 (-) 4.3 (2.42)" a Two days of germination period under the same treatment conditions is included in the total seedling age.…”

Section: Discussionmentioning

confidence: 99%

Effects of Benlate 50 DF on Microtubules of Cucumber Root Tip Cells and on Growth of Cucumber Seedlings

Woo

Wick

1995

American Journal of Botany

View full text Add to dashboard Cite

The aim of this study was to determine whether the fungicide Benlate 50 DF has adverse effects on the microtubules of cucumber root tip cells and on growth ofthe young seedlings. Germinating cucumber seeds and young seedlings were exposed to six concentrations of Benlate ranging from I mg/liter to l Gg/liter. Although seed germination was not affected by Benlate, seedling growth, especially number and length of branch roots, was reduced in proportion to the concentration of Benlate and length of treatment. Significantly lower mitotic indices were obtained from root tips exposed to 0.1, 0.6, I, or 10 g/liter of Benlate. By means of immunofluorescence microscopy, the major microtubule arrays of root tip cells exposed to Benlate were compared and contrasted to those of controls. A few abnormalities in microtubule arrays were found at or after cytokinesis, and were of two types: I) persistence of phragmoplast microtubules; and 2) presence of an array of microtubules between two recently divided daughter nuclei in the plane that would normally be occupied by the new cell wall. These abnormalities are somewhat similar to those induced by caffeine, and, as with caffeine treatment, possibly reflect impaired or/and incomplete cytokinesis that results in production of binucleate cells. A few binucleate cells were observed in root tips exposed to l O g/liter of Benlate.

show abstract

“…It is well known that, without detectably interfering with phragmoplast microtubules during cytokinesis, caffeine prevents the completion of new cell wall formation (Pickett-Heaps, 1969;Roper and Roper, 1977;Pareyre, Lasselain, and Deysson, 1979;Bonsignore and Hepler, 1985;Galatis and Apostolakos, 1991). For small plots, the instructions are to use half of the concentration listed for larger acreage spraying, but to apply it at two times the volume per acre oflarger areas, essentially drenching the plants.…”

Section: Discussionmentioning

confidence: 99%

Effects of Benlate 50 DF on microtubules of cucumber root tip cells and on growth of cucumber seedlings

Woo¹,

Wick²

1995

American J of Botany

View full text Add to dashboard Cite

The aim of this study was to determine whether the fungicide Benlate 50 DF has adverse effects on the microtubules of cucumber root tip cells and on growth of the young seedlings. Germinating cucumber seeds and young seedlings were exposed to six concentrations of Benlate ranging from 1 mg/liter to 10 g/liter. Although seed germination was not affected by Benlate, seedling growth, especially number and length of branch roots, was reduced in proportion to the concentration of Benlate and length of treatment. Significantly lower mitotic indices were obtained from root tips exposed to 0.1, 0.6, 1, or 10 g/liter of Benlate. By means of immunofluorescence microscopy, the major microtubule arrays of root tip cells exposed to Benlate were compared and contrasted to those of controls. A few abnormalities in microtubule arrays were found at or after cytokinesis, and were of two types: 1) persistence of phragmoplast microtubules; and 2) presence of an array of microtubules between two recently divided daughter nuclei in the plane that would normally be occupied by the new cell wall. These abnormalities are somewhat similar to those induced by caffeine, and, as with caffeine treatment, possibly reflect impaired or/and incomplete cytokinesis that results in production of binucleate cells. A few binucleate cells were observed in root tips exposed to 10 g/liter of Benlate.

show abstract

Centripetal wall formation in roots ofVicia faba after caffeine treatment

Cited by 22 publications

References 20 publications

Unique cell wall abnormalities in the putative phosphoinositide phosphatase mutant AtSAC9

Unique cell wall abnormalities in the putative phosphoinositide phosphatase mutant AtSAC9

Effects of Benlate 50 DF on Microtubules of Cucumber Root Tip Cells and on Growth of Cucumber Seedlings

Effects of Benlate 50 DF on microtubules of cucumber root tip cells and on growth of cucumber seedlings

Contact Info

Product

Resources

About