Centrin-Mediated Cell Motility in Algae

Melkonian, Michael; Beech, Peter L.; Katsaros, Christos; Schulze, Dorothee

doi:10.1007/978-1-4615-9683-7_6

Cited by 66 publications

(59 citation statements)

References 99 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3B, lane 3 and 4). This result was coincided with the immunofluorescence data that caltractin homologues are found in every eukaryotic lineage investigated in association with a variety of flagellar or basal body-associated structures (Melkonian et al 1992). However, there were another two immunoreactive polypeptides in the supernatant fraction (Fig.…”

supporting

confidence: 62%

“…Caltractin, also known as centrin, is considered to be a ubiquitous component of the basal body/centriole complex in eukaryotic cells (Baron et al 1992, Melkonian et al 1992. It is a 20 kDa calcium-modulated EF-hand protein structurally homologous to calmodulin (Salisbury et al 1984, Huang et al 1988, Lee et al 1991, Lee and Huang 1993, Ogawa and Shimizu 1993, Errabolu et al 1994).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Molecular Cloning and Characterization of a cDNA Encoding Caltractin from Dunaliella salina

Ko¹,

Kim²,

Lee³

1999

Plant and Cell Physiology

View full text Add to dashboard Cite

We cloned a cDNA encoding caltractin, a 20 kDa calcium-binding protein, from Dunaliella salina (DSCALT). The Ca 2+ -bound mobility shift detected in Chlamydomonas caltractin was hardly detectable in DSCALT. Also, some differences were found in the electrophoretic mobility between the native DSCALT and the bacterial-expressed DSCALT. This difference may have resulted from the posttranslational modification. Immunoblot analysis revealed that this protein might be localized mainly in the basal body complex, the major microtubule organizing center (MTOC) in D. salina and the functional homologue of the centrosome in the animal cell.

show abstract

supporting

confidence: 62%

mentioning

confidence: 99%

Molecular Cloning and Characterization of a cDNA Encoding Caltractin from Dunaliella salina

Ko¹,

Kim²,

Lee³

1999

Plant and Cell Physiology

View full text Add to dashboard Cite

show abstract

“…The star-shaped fibers that characterize the stellate pattern of other green plants label with antibodies to centrin (Hoffman et al, 1994;Salisbury, 1995;Melkonian et al, 1992). In biflagellated lycophytes, fibrous connectors between basal bodies and spline are also centrin-containing .…”

Section: Mtocs In Developing Sperm Cellsmentioning

confidence: 97%

Microtubule organizing centers and the origin of centrioles during spermatogenesis in the PteridophytePhylloglossum

Renzaglia

Maden

2000

Microsc. Res. Tech.

View full text Add to dashboard Cite

“…The calciumbinding domains are co-linear to those of calmodulin and congruent with the CTER subfamilies of EF-hand proteins [Nakayama and Kretsinger, 1994]. Centrin can be found in microtubule organizing centers (MTOC) of diverse organisms from protists to humans [Melkonian 1992;Schiebel and Bornens 1995]. It was first identified as a major component in isolated striated flagellar roots (SFR), which consist of filaments thought to be involved in basal body localization, orientation, and segregation [Wright et al, 1985] of the green alga Tetraselmis striata [Salisbury et al, 1984].…”

Section: Introductionmentioning

confidence: 99%

In vivo localization of centrin in the green alga Chlamydomonas reinhardtii

Ruiz-Binder¹,

Geimer²,

Melkonian³

2002

Cell Motil. Cytoskeleton

Self Cite

View full text Add to dashboard Cite

The green alga Chlamydomonas reinhardtii has been used as a model system to study flagellar assembly, centriole assembly, and cell cycle events. These processes are dynamic. Therefore, protein targeting and protein-protein interactions should be evaluated in vivo. To be able to study dynamic processes in C. reinhardtii in vivo, we have explored the use of the green fluorescent protein (GFP). A construct containing a fusion of centrin and GFP was incorporated into the genome as a single copy. The selected clone shows expression in 25-50% of the cells. Centrin-GFP was targeted in vivo to the nuclear basal body connectors and the distal connecting fibers. At the electron microscopic level, it was also localized to the flagellar transitional regions. EM data of transformants indicate that there are some abnormalities in the centrin-containing structures. The transitional region consists of only the transverse septum or has lesions in the H-piece. The distal connecting fibers are thinner and their characteristic crossbands seem to be incomplete. Deflagellation is not affected since more than 95% of the cells deflagellate. Also basal body segregation is not affected since cells with an abnormal flagellar number were not detected. Functional studies of the centrin-GFP fusion show the characteristic calcium-induced mobility shift in SDS-PAGE. Immunofluorescence revealed that during cell division, centrin-GFP remains associated with the basal bodies. In vivo localization of the fusion protein during cell division shows that in metaphase centrin-GFP appears as two opposing spots located close to the spindle poles. The distance between the spots increases as the cells progress through anaphase and then decreases during telophase. GFP is a useful tool to study dynamic processes in the cytoskeleton of C. reinhardtii.

show abstract

Centrin-Mediated Cell Motility in Algae

Cited by 66 publications

References 99 publications

Molecular Cloning and Characterization of a cDNA Encoding Caltractin from Dunaliella salina

Molecular Cloning and Characterization of a cDNA Encoding Caltractin from Dunaliella salina

Microtubule organizing centers and the origin of centrioles during spermatogenesis in the PteridophytePhylloglossum

In vivo localization of centrin in the green alga Chlamydomonas reinhardtii

Contact Info

Product

Resources

About