Centrifuge Polarizing Microscope with Dual Specimen Chambers and Injection Ports

Knudson, R. A.; Inoué, Shinya; Goda, Makoto

doi:10.2307/1543339

Cited by 2 publications

(2 citation statements)

References 2 publications

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“…That provides the trigger for viewing chamber B. Thus, one can instantly select to see the specimen in chamber A or B simply by flipping the BS (2). The inside dimension of the specimen chamber is 8 mm long (column height), 3 mm wide, and 0.5 mm thick.…”

Section: Resultsmentioning

confidence: 99%

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human andAmphiumared blood cells

Hoffman¹,

Inoué²

2008

Collected Works of Shinya Inoué

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This paper describes changes that occur in human and Amphiuma red blood cells observed during centrifugation with a special microscope. Dilute suspensions of cells were layered, in a centrifuge chamber, above an osmotically matched dense solution, containing Nycodenz, Ficoll, or Percoll (Pharmacia) that formed a density gradient that allowed the cells to slowly settle to an equilibrium position. Biconcave human red blood cells moved downward at low forces with minimum wobble. The cells oriented vertically when the force field was increased and Hb sedimented as the lower part of each cell became bulged and assumed a ''baglike'' shape. The upper centripetal portion of the cell became thinner and remained biconcave. These changes occurred rapidly and were completely reversible upon lowering the centrifugal force. Bag-shaped cells, upon touching red cells in rouleau, immediately reverted to biconcave disks as they flipped onto a stack. Amphiuma red cells displayed a different type of reversible stratification and deformation at high force fields. Here the cells became stretched, with the nucleus now moving centrifugally, the Hb moving centripetally, and the bottom of the cells becoming thinner and clear. Nevertheless, the distribution of the marginal bands at the cells' rim was unchanged. We conclude that centrifugation, per se, while changing a red cell's shape and the distribution of its intracellular constituents, does so in a completely reversible manner. Centrifugation of red cells harboring altered or missing structural elements could provide information on shape determinants that are still unexplained. centrifuge polarization microscope ͉ Hb sedimentation ͉ shape deformations Conflict of interest statement: No conflicts declared.

show abstract

Section: Resultsmentioning

confidence: 99%

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human andAmphiumared blood cells

Hoffman¹,

Inoué²

2008

Collected Works of Shinya Inoué

Self Cite

View full text Add to dashboard Cite

show abstract

“…These studies were carried out by use of a newly developed centrifuge polarizing microscope (CPM) (see Fig. 1), that provides, at high resolution, direct observation of the red blood cells, at speeds of up to 11,000 rpm (1,2). It is known that centrifugation, per se, has essentially no effect on shape or volume when comparisons are made of human red cells before and after centrifugation.…”

mentioning

confidence: 99%

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human and Amphiuma red blood cells

Hoffman

Inoué²

2006

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

show abstract

Centrifuge Polarizing Microscope with Dual Specimen Chambers and Injection Ports

Cited by 2 publications

References 2 publications

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human andAmphiumared blood cells

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human andAmphiumared blood cells

Directly observed reversible shape changes and hemoglobin stratification during centrifugation of human and Amphiuma red blood cells

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