Centrifugation of Rickettsiae and Viruses Onto Cells and Its Effect on Infection

Weiss, Emilio; Dressler, Harry R.

doi:10.3181/00379727-103-25637

Cited by 71 publications

(36 citation statements)

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“…Psittacosis virus antiserum obtained from immunized turkeys was conjugated with fluorescein isothiocyanate by the method of Riggs et al 8 The conjugate was adsorbed twice with liver, kidney, heart, and muscle powder of rabbit origin and once with spleen and bone marrow powder. For staining, the conjugate was used as a 1:10 dilution.…”

Section: E Antiserum Conjugate and Technique Of Immunofluorescent Stmentioning

confidence: 99%

Quantitative assay of psittacosis virus by the fluorescent cell-counting technique

Hahon¹,

Nakamura²

1964

Virology

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Section: E Antiserum Conjugate and Technique Of Immunofluorescent Stmentioning

confidence: 99%

Quantitative assay of psittacosis virus by the fluorescent cell-counting technique

Hahon¹,

Nakamura²

1964

Virology

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“…The development of simple methods for isolating chlamydia A directly from the human genital tract or eye has been remarkably slow, although it has been known for many years (Furness, Graham & Reeve, 1960) that certain 'fast' laboratory-adapted strains, isolated and serially passaged in the yolk sac of chick embryos, could be grown in tissue culture by methods in general use in virology laboratories, and that a wide variety of cell lines could be used for this purpose (Blyth & Taverne, 1974). Adsorption of chlamydia to tissue culture cells is inefficient and slow, but this can be overcome to some extent by centrifuging the infected inoculum onto the monolayer, as with rickettsias (Weiss & Dressler, 1960) or by pretreatment of tissue cultures with DEAE-dextran (Rota & Nichols, 1973). The main problem, however, has been the lack of precise information on the optimum conditions for the growth of genital strains of chlamydia A in tissue culture.…”

Section: Introductionmentioning

confidence: 99%

Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents)

Johnson

Hobson

1976

J. Hyg.

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SUMMARYNormal non-irradiated McCoy cell cultures provide a sensitive and reproducible method for the isolation of oculo-genital strains, of chlamydia A directly from human secretions and for laboratory studies with these agents. Since September 1973, chlamydia have been isolated from 175 of 562 women (32.1 %) attending venereal disease clinics. Freshly isolated and low passage strains have been used to determine the importance of centrifugation, constitution and pH of the tissue culture medium, and the temperature of incubation in controlling the efficiency of plating in the method.

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“…The principles of this technique are based on the inoculation of clinical specimens by low-speed centrifugation on confluent cell monolayers seeded onto 1-cm 2 -round coverslips and incubated with 1 ml of culture medium in a shell vial. The centrifugation step is critical because this step enhances the attachment and penetration of the bacteria in cells (354). The sensitivity of this cell culture technique is increased by the small surface area of the coverslip, which contains cells that enhance the ratio of the number of intracellular bacteria to the number of cells and that allow more efficient recovery.…”

Section: Cell Culturementioning

confidence: 99%

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

et al. 2015

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SUMMARY A pure bacterial culture remains essential for the study of its virulence, its antibiotic susceptibility, and its genome sequence in order to facilitate the understanding and treatment of caused diseases. The first culture conditions empirically varied incubation time, nutrients, atmosphere, and temperature; culture was then gradually abandoned in favor of molecular methods. The rebirth of culture in clinical microbiology was prompted by microbiologists specializing in intracellular bacteria. The shell vial procedure allowed the culture of new species of Rickettsia . The design of axenic media for growing fastidious bacteria such as Tropheryma whipplei and Coxiella burnetii and the ability of amoebal coculture to discover new bacteria constituted major advances. Strong efforts associating optimized culture media, detection methods, and a microaerophilic atmosphere allowed a dramatic decrease of the time of Mycobacterium tuberculosis culture. The use of a new versatile medium allowed an extension of the repertoire of archaea. Finally, to optimize the culture of anaerobes in routine bacteriology laboratories, the addition of antioxidants in culture media under an aerobic atmosphere allowed the growth of strictly anaerobic species. Nevertheless, among usual bacterial pathogens, the development of axenic media for the culture of Treponema pallidum or Mycobacterium leprae remains an important challenge that the patience and innovations of cultivators will enable them to overcome.

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Centrifugation of Rickettsiae and Viruses Onto Cells and Its Effect on Infection

Cited by 71 publications

References 0 publications

Quantitative assay of psittacosis virus by the fluorescent cell-counting technique

Quantitative assay of psittacosis virus by the fluorescent cell-counting technique

Factors affecting the sensitivity of replicating McCoy cells in the isolation and growth of chlamydia A (TRIC agents)

Current and Past Strategies for Bacterial Culture in Clinical Microbiology

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