Centrifugation-induced production of triacylglycerols in Chlamydomonas reinhardtii

Kato, Naohiro; Lum, Tony; Légeret, Bertrand; Li‐Beisson, Yonghua; Ndathe, Ruth

doi:10.1016/j.biteb.2018.10.003

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…Previously (Coragliotti et al ., 2011), we had achieved this by centrifuging cells from all time points and resuspending them in fresh TAP to avoid any possible effect of nutrients coming from different volumes of fresh medium between samples. However, we modified this procedure as it was recently reported that centrifugation induces lipid production (Kato et al ., 2019). The modified protocol involved adding, every hour, the volume of fresh medium necessary to revert the cell density to the value at the end of the dark period, which we had previously determined that did not impact the accumulation of the selected target molecules at the times points analyzed (unpublished results).…”

Section: Methodsmentioning

confidence: 99%

Expression of Chlamydomonas reinhardtii chloroplast diacylglycerol acyltransferase 3 is induced by light in concert with triacylglycerol accumulation

et al. 2022

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Considerable progress has been made towards the understanding of triacylglycerol (TAG) accumulation in algae. One key aspect is finding conditions that trigger TAG production without reducing cell division. Previously, we identified a soluble diacylglycerol acyltransferase (DGAT), related to plant DGAT3, with heterologous DGAT activity. In this work, we demonstrate that Chlamydomonas reinhardtii DGAT3 localizes to the chloroplast and that its expression is induced by light, in correspondence with TAG accumulation. Dgat3 mRNAs and TAGs increase in both wild-type and starch-deficient cells grown with acetate upon transferring them from dark or low light to higher light levels, albeit affected by the particularities of each strain. The response of dgat3 mRNAs and TAGs to light depends on the pre-existing levels of TAGs, suggesting the existence of a negative regulatory loop in the synthesis pathway, although an effect of TAG turnover cannot be ruled out. Altogether, these results hint towards a possible role of DGAT3 in light-dependent TAG accumulation in C. reinhardtii.

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Section: Methodsmentioning

confidence: 99%

Expression of Chlamydomonas reinhardtii chloroplast diacylglycerol acyltransferase 3 is induced by light in concert with triacylglycerol accumulation

et al. 2022

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show abstract

“…Previously (Coragliotti et al, 2011), we had done this by centrifuging cells from all time points and resuspending them in fresh TAP to avoid a possible effect of nutrients coming from different volumes of fresh medium between samples. However, we modified this procedure since it was recently reported that centrifugation induces lipid production (Kato et al, 2019). The modified protocol consisted in adding, every hour, the volume of fresh medium necessary to revert the cell density to the value at the end of the dark, which we had previously determined that did not impact the accumulation of the selected target molecules at the times points analyzed (unpublished results).…”

Section: Chlamydomonas Reinhardtii Strains and Culture Conditionsmentioning

confidence: 99%

Expression ofChlamydomonas reinhardtiichloroplast diacylglycerol acyltransferase-3 is activated by light in concert with triacylglycerol accumulation

Carro

Soto

Mamone

et al. 2021

Preprint

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Considerable progress has been made towards the understanding of triacylglycerol (TAG) accumulation in algae. One key aspect is finding conditions that trigger TAG production without reducing cell division. Previously, we identified a soluble diacylglycerol acyltransferase (DGAT), related to plant DGAT3, with heterologous DGAT activity. In this work, we demonstrate that Chlamydomonas reinhardtii DGAT3 localizes to the chloroplast and its expression is activated by light, in correspondence with TAG accumulation. Dgat3 mRNAs and TAGs increased in both wild type and starch-deficient cells grown with acetate upon transferring them from dark or low light to higher light. Light-activated DGAT3 expression and TAG accumulation depended on the preexisting levels of TAGs, suggesting the existence of a regulatory loop. These results indicate that DGAT3 could be responsible, at least in part, for light-dependent TAG accumulation. Moreover, our results present DGAT3 as a promising target of future studies oriented to the industrial applications of TAGs.

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A complex metabolic rearrangement towards the accumulation of glycerol and sugars consequence of a proteome remodeling is required for the survival of Chlamydomonas reinhardtii growing under osmotic stress

Colina

Carbó

Cañal

et al. 2020

Environmental and Experimental Botany

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Centrifugation-induced production of triacylglycerols in Chlamydomonas reinhardtii

Cited by 4 publications

References 25 publications

Expression of Chlamydomonas reinhardtii chloroplast diacylglycerol acyltransferase 3 is induced by light in concert with triacylglycerol accumulation

Expression of Chlamydomonas reinhardtii chloroplast diacylglycerol acyltransferase 3 is induced by light in concert with triacylglycerol accumulation

Expression ofChlamydomonas reinhardtiichloroplast diacylglycerol acyltransferase-3 is activated by light in concert with triacylglycerol accumulation

A complex metabolic rearrangement towards the accumulation of glycerol and sugars consequence of a proteome remodeling is required for the survival of Chlamydomonas reinhardtii growing under osmotic stress

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