Centrifugal partition chromatography for purification of Cannabidiol from Cannabis sativa

Lahoutifard-Henry, N; Pahnke, L; Quemener, Céline Le; Audo, Grégoire; Simdon, L

doi:10.1055/s-0039-1697319

Cited by 1 publication

(2 citation statements)

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“…In recent years, numerous therapeutic effects of C. sativa have been associated with Δ 9 ‐tetrahydrocannabinol (Δ 9 ‐THC) and non‐psychotropic cannabinoids including CBD 13 . CPC is highly selective to guarantee industrial‐grade preparative separation of CBD from crude C. sativa extract, with CBD purity higher than 95% 24–26 . The optimal biphasic solvent system generally used is alkane–methanol–water (5:4:1, v/v/v) in descending mode.…”

Section: Resultsmentioning

confidence: 99%

“…13 CPC is highly selective to guarantee industrial-grade preparative separation of CBD from crude C. sativa extract, with CBD purity higher than 95%. [24][25][26] The optimal biphasic solvent system generally used is alkane-methanol-water (5:4:1, v/v/v) in descending mode. Herein, we proposed to substitute alkane and methanol by HMDSO and ethanol, respectively.…”

Section: Cannabidiol (Cbd)mentioning

confidence: 99%

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Investigation of alternative two‐phase solvent systems for purification of natural products by centrifugal partition chromatography

Michel,

Loyet,

Boulho

et al. 2023

Phytochemical Analysis

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IntroductionCentrifugal partition chromatography (CPC) is a liquid–liquid chromatography characterised by its solvent flexibility. The compounds undergoing separation are subjected to a continuous partition process between two immiscible phases in a column space free of solid support. In the context of green chemistry, it is important to substitute halogenated and petroleum‐based solvents commonly used in purification processes.ObjectivesThe main goal of the current study was to replace classical solvents used in CPC (e.g., hexane and methanol) by green and renewable alternatives.MethodsSolvents were first selected based on literature. Their commercial availability, price, recyclability, toxicity and ability to form two phases were particularly sought after.Key findingsThe new two‐phase solvent systems were evaluated for the purification of two compounds of interest: piperine and cannabidiol. Using these alternative two‐phase solvent systems allows us to isolate natural products with a high purity level (> 95%).ConclusionSubstituting petroleum‐based solvents with bio‐sourced, renewable alternatives reduces the environmental impact of CPC. Herein, new biphasic solvent systems were built using hexamethyldisiloxane, ethyl isobutyrate and 2‐methyl tetrahydrofuran in combination with ethanol and water. Furthermore, this research provides a scientific basis for developing new and sustainable solvent systems in CPC.

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Section: Resultsmentioning

confidence: 99%