Centrifugal microfluidic-based multiplex recombinase polymerase amplification assay for rapid detection of SARS-CoV-2

Li, Ruoxu; Ning, Shunzong; Ren, Xiaodong; Sun, Xian-Ge; Li, Wenman; Li, Yuwei; Li, Jin; Chen, Chen; Wang, Hong; Lu, Weiping; Deng, Shaoli; Huang, Qing

doi:10.1016/j.isci.2023.106245

Cited by 6 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A centrifugal microfluidic platform was proposed to detect SARS-CoV-2 rapidly [62]. A multiplex assay targeting the E, N, and ORF1ab genes of SARS-CoV-2 was realized using RT-RPA and fluorescence monitoring.…”

Section: Fluorescence-based Detection Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Ngoc,

Lee

2024

Biosensors

View full text Add to dashboard Cite

Ribonucleic acid (RNA) viruses are one of the major classes of pathogens that cause human diseases. The conventional method to detect RNA viruses is real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR), but it has some limitations. It is expensive and time-consuming, with infrastructure and trained personnel requirements. Its high throughput requires sophisticated automation and large-scale infrastructure. Isothermal amplification methods have been explored as an alternative to address these challenges. These methods are rapid, user-friendly, low-cost, can be performed in less specialized settings, and are highly accurate for detecting RNA viruses. Microfluidic technology provides an ideal platform for performing virus diagnostic tests, including sample preparation, immunoassays, and nucleic acid-based assays. Among these techniques, nucleic acid isothermal amplification methods have been widely integrated with microfluidic platforms for RNA virus detection owing to their simplicity, sensitivity, selectivity, and short analysis time. This review summarizes some common isothermal amplification methods for RNA viruses. It also describes commercialized devices and kits that use isothermal amplification techniques for SARS-CoV-2 detection. Furthermore, the most recent applications of isothermal amplification-based microfluidic platforms for RNA virus detection are discussed in this article.

show abstract

Section: Fluorescence-based Detection Methodsmentioning

confidence: 99%

“…In the future, integrating sample preparation on a chip platform should be performed. Secondly, high-throughput screening for targets remained limited [62]. Figure 4 illustrates the strategy for using centrifugal microfluidic devices to detect multiple SARS-CoV-2 genes.…”

Section: Fluorescence-based Detection Methodsmentioning

confidence: 99%

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Ngoc,

Lee

2024

Biosensors

View full text Add to dashboard Cite

show abstract

“…Additionally, 3D bioprinting offers a promising solution for large-scale tissue construction, blood vessel maturation, organ transplantation, and regeneration research [ 80 , 139 , 140 ]. Integrating biosensors, multiplex, and artificial intelligence setups into chips can provide real-time online monitoring of the behavior of SARS-CoV2, including viral infection and transmission, microenvironment changes, and organ function [ 141 , 142 ]. Finally, on-chip multi-omics and high-resolution imaging devices can be used to analyze ultra-early-stage viral infection detection, virus screening, organopathy, and secondary and systemic diseases [ [143] , [144] , [145] ].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Microfluidic strategies for biomimetic lung chip establishment and SARS-CoV2 study

Wang,

Wen,

Zhu

et al. 2024

Materials Today Bio

View full text Add to dashboard Cite

“…Для обнаружения SARS-CoV-2 предложен чип на основе центробежной микрофлюидики, сочетающий ОТ-РПА и флуоресцентную детекцию по конечной точке [62]. Микрофлюидный чип в форме предметного стекла для микроскопа позволял одновременно обнаруживать наличие 3 вирусных мишеней и 1 мишень ДНК человека за 30 мин.…”

Section: автономные портативные устройстваunclassified

Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases

Bondareva,

Baturin,

Mironova

2024

Journal of microbiology, epidemiology and immunobiology

View full text Add to dashboard Cite

Isothermal amplification techniques have been actively developed in recent years and are gradually introduced into the range of methods for infectious disease diagnostics. One of the fastest isothermal methods is recombinase polymerase amplification (RPA). This review contains information about the principle of RPA, the role of individual reaction components and primer design considerations. It provides information on characteristics of various methods of RPA results detection, effects of inhibitors, temperature and agitation on the efficiency of reaction. Approaches to quantitative and multiplex RPA are described, as well as some variants of portable devices designed to identify infectious agents. The conclusion summarizes advantages and disadvantages of RPA in comparison with other amplification methods.

show abstract

Centrifugal microfluidic-based multiplex recombinase polymerase amplification assay for rapid detection of SARS-CoV-2

Cited by 6 publications

References 53 publications

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Current Trends in RNA Virus Detection via Nucleic Acid Isothermal Amplification-Based Platforms

Microfluidic strategies for biomimetic lung chip establishment and SARS-CoV2 study

Recombinase polymerase amplification: method’s characteristics and applications in diagnostics of infectious diseases

Contact Info

Product

Resources

About