Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro

Pietroboni, Glenys R.; Harnett, Gerald B.; Bucens, Marion R.

doi:10.1016/0166-0934(89)90010-4

Cited by 48 publications

(27 citation statements)

References 8 publications

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“…The use of centrifugally enhanced inoculation would have resulted in an effective multiplicity of 10 TCID &! virus\cell (Pietroboni et al, 1989). During the adsorption period, cells and virus were initially incubated at 4 mC for 2 h, before being incubated at 37 mC to promote synchronous virus entry into cells.…”

Section: Mrna Levels After Cell-free Hiv Infectionmentioning

confidence: 99%

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Kinetics of viral RNA synthesis following cell-to-cell transmission of human immunodeficiency virus type 1.

Davis

Burrell

1997

Journal of General Virology

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The temporal appearance and levels of human immunodeficiency virus type 1 (HIV-1) tat, rev, nef, env and gag mRNA species were examined using a synchronized, one-step, cell-to-cell HIV-1 infection model involving HUT-78 cells and HIV-1 persistently infected H3B cells. Individual mRNAs were quantified by RT-PCR using RNA standards transcribed in vitro from cDNA clones. Consistent with an infection that produces high yields of virus, significant levels of env and gag mRNAs were detected in the cytoplasm of infected cells late in the infection cycle. However, at no time after infection did levels of tat, rev and nef mRNA, which encode the regulatory proteins of HIV-1, exceed their levels present in the persistently infected virus donor H3B cells. The

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Section: Mrna Levels After Cell-free Hiv Infectionmentioning

confidence: 99%

“…HuT-78 cells were infected with cell-free virus at a nominal multiplicity of 1 TCID &! virus per cell using a centrifugal enhancement technique Pietroboni et al, 1989). RNA was extracted from cells immediately after exposure to the virus (0 h) and 4, 8, 12, 16 and 24 h later.…”

Section: Virus Infectionmentioning

confidence: 99%

Kinetics of viral RNA synthesis following cell-to-cell transmission of human immunodeficiency virus type 1.

Davis

Burrell

1997

Journal of General Virology

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“…The centrifugal enhancement of infection has been described for human and murine cytomegaloviruses (Ho et al, 1993;Hodgkin et al, 1988;Hudson et al, 1976;Osborn and Walker, 1968), retroviruses (Bahnson et al, 1995;Chuck et al, 1996;Ho et al, 1993;Kotani et al, 1994;Pietroboni et al, 1989), bluetongue virus (Sundin and Mecham, 1989), adenoviruses (Nyberg-Hoffman et al, 1997), human herpesvirus type 6 (Pietroboni et al, 1989) and influenza A virus (Reading et al, 2001). This study evaluated the impact of centrifugation on HCV JFH1 infection of human hepatocytes.…”

Section: Introductionmentioning

confidence: 99%

Centrifugal enhancement of hepatitis C virus infection of human hepatocytes

Wang

et al. 2008

Journal of Virological Methods

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Hepatitis C virus (HCV) is a human pathogen associated with chronic liver disease. Recently, the cell culture systems supporting complete replication and production of HCV genotype 2a (JFH1) have been established. This study investigated the effect of low-speed centrifugation on HCV JFH1 infection of human hepatocytes (Huh7.5.1). Higher levels of HCV RNA expression were observed in Huh7.5.1 cells infected with centrifugal inoculation of HCV JFH1 than those in the control cells. This increased HCV RNA expression was associated with the elevated expression of HCV NS3 protein in the hepatocytes. The centrifugal enhancement of HCV infection was time and speed dependent. However, the enhancement was not observed when centrifugation was performed before or after HCV infection. In addition, there was no association between centrifugal enhancement and the expression of HCV entry receptors (CD81 and claudin-1) and intracellular IFN-α in the hepatocytes. These data indicate that centrifugal inoculation is a useful tool for increasing the efficiency of HCV infection and replication in the target cells in vitro.

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“…The centrifugation-enhancement technique used has been reported to increase the infectious titre of the virus 10-fold in comparison with the conventional, non-enhanced infection method (Pietroboni et al, 1989), and thus the actual m.o.i, in the experiment would be approximately 10. Fig.…”

mentioning

confidence: 99%

De novo reverse transcription is a crucial event in cell-to-cell transmission of human immunodeficiency virus

Kuiper

Stephenson

Burrell

1992

Journal of General Virology

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Centrifugal enhancement of human immunodeficiency virus (HIV) and human herpesvirus type 6 (HHV-6) infection in vitro

Cited by 48 publications

References 8 publications

Kinetics of viral RNA synthesis following cell-to-cell transmission of human immunodeficiency virus type 1.

Kinetics of viral RNA synthesis following cell-to-cell transmission of human immunodeficiency virus type 1.

Centrifugal enhancement of hepatitis C virus infection of human hepatocytes

De novo reverse transcription is a crucial event in cell-to-cell transmission of human immunodeficiency virus

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