Central Spindle Assembly and Cytokinesis Require a Kinesin-like Protein/RhoGAP Complex with Microtubule Bundling Activity

Mishima, Masanori; Kaitna, Susanne; Glotzer, Michael

doi:10.1016/s1534-5807(01)00110-1

Cited by 464 publications

(594 citation statements)

References 32 publications

Supporting

Mentioning

559

Contrasting

Unclassified

Order By: Relevance

“…This is reflected in the different strategies S. pombe and animal cells follow to set the division plane according to DNA location inside the cell. In metazoan mitotic cells, microtubules from the spindle midzone, organized in-between the two sets of segregating chromosomes, contact the cell cortex at the future cleavage site and deliver the centralspindlin complex which in turn activates the small GTPase RhoA [Mishima et al, 2002], a key step in contractile ring assembly and furrow ingression, while polar microtubules inhibit cortical contractility. In contrast, as discussed in more details below, in fission yeast, the microtubule cytoskeleton plays an indirect role in division plane selection by positioning the nucleus in the cell center and distributing polarity cues to the cell tips.…”

Section: Review Articlementioning

confidence: 99%

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

Cytoskeleton

View full text Add to dashboard Cite

Cell division is a critical and irreversible step in the cell cycle. The strategies that cells follow to regulate the position of the division plane must take into account the global geometry of the cell as well as position of the genetic material to ensure its accurate segregation into daughter cells of a given cell shape and size. Along the years, research on Schizosaccharomyces pombe, a wellrecognized model organism for cell division studies has allowed a detailed molecular understanding of the spatial mechanisms regulating cytokinesis. Division plane position in this unicellular rod-shaped organism, which divides by the assembly and constriction of a medially placed actomyosin ring, largely depends on the anillinlike protein Mid1. Therefore, the major pathways controlling the position of the division plane converge on Mid1. In this review, we make an overview of the studies that have deciphered how Mid1 localization and scaffolding activities are controlled over the cell cycle to ensure the symmetrical division of fission yeast cells. These studies have revealed new mechanisms generating spatial information based on nuclear shuttling of the division plane factor Mid1 and on the establishment of cortical inhibitory gradients of the cell polarity kinase Pom1. V C 2012 Wiley Periodicals, Inc Key Words: cytokinesis, fission yeast, contractile ring, Mid1, cell division Introduction C ytokinesis is the final step of cell division that physically separates the daughter cells at the end of the cell cycle. It is a universal process, essential for cell proliferation, for the survival of unicellular organisms or for the development of multicellular organisms, whose major features have been well conserved along evolution. As an irreversible event, cytokinesis is under tight spatial and temporal regulation. Defective cytokinesis can indeed alter cell geometry or size, perturb cellular organization within tissues or prevent the accurate transmission of the genetic material, producing aneuploid cells, which can affect the survival of unicellular species or favor cancer and tumor progression in animal species.Cytokinesis is first strictly coordinated with mitotic progression in order to successfully fulfill chromosome segregation. Checkpoint mechanisms monitoring the function of the mitotic apparatus can halt cells in metaphase, which prevents cytokinesis until actual chromosome segregation in anaphase.Second, spatial regulatory pathways define the position of the division plane according to the position of the nucleus at mitotic entry, or of the mitotic apparatus, perpendicular to the axis of chromosome segregation. This ensures the efficient segregation of the genetic material and allows to control the geometry of the daughter cells in response to morphogenetic programs. Cell division can indeed be symmetric or asymmetric to allow the generation of daughter cells of different sizes. Orientation of the division plane is also modulated to properly position the daughter cells inside embryos or tissues and to permit the asymm...

show abstract

Section: Review Articlementioning

confidence: 99%

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Rincón

Paoletti

2012

Cytoskeleton

View full text Add to dashboard Cite

show abstract

“…Centraspindlin is a heterotetramer that consists of MKLP-1 and CYK-4, which contains a GTPase-activating protein (GAP) domain for Rho GTPases. As a whole heterotetramer, containing two molecules of MKLP-1 and two molecules of CYK-4 RhoGAP, it promotes microtubule bundling in vitro [15] and RhoA regulation and recruits regulators of abscission [2].…”

Section: Early Cytokinesismentioning

confidence: 99%

“…Microtubule-associated proteins PRC1 [13,14] Centralspindlin complex CYK-4 [2,15] MKLP1 [2,15] Chromosome passenger complex INCENP [16,17] Survinin [16,17] Borealin [16,17] Aurora B [16,17] ESRCTs and associated proteins CEP55 [30][31][32] TSG101 [31,32] ALIX [31,32] CHMP1B [33] Spastin [33] CHMP4B [31,32,34] FYVE-CENT [34] TTC19 [34] Kinesins KIF4A [14] MKLP2 [17] MPP1 [22] KIF14 [23] KIF13A [34] Additional proteins NuSAP [18] Orbit [19] ASP [20] TBCD [21] ECT2 [24] FIP3 [25] PLK1 [26,27] Septins [28] Anillin [29] during anaphase and cytokinesis and is important for the last step of abscission. CEP55 binds directly MKLP1 and is controlled by centralspindlin, since knockdown of centralspindlin abolishes CEP55 from the midbody [30].…”

Section: The Abscission Step Of Cytokinesismentioning

confidence: 99%

Cytokinesis and cancer

Sagona

Stenmark

2010

FEBS Letters

View full text Add to dashboard Cite

a b s t r a c tCytokinesis is the final stage of cell division during which the two daughter cells separate completely. Although less well understood than some of the earlier phases of the cell cycle, recent discoveries have shed light on the mechanisms that orchestrate this process, including cleavage furrow formation, midbody maturation and abscission. One of the reasons why research on cytokinesis has been attracting increasing attention is the concept that failure of this process in mammals is associated with carcinogenesis. In this minireview, we will discuss the possible links between cytokinesis and cancer, and highlight key mechanisms that connect these processes.

show abstract

“…(34)(35)(36) Ect2 associates with MgcRacGAP, (20)(21)(22)(23)37) a component of the centralspindlin complex. (38) The other centralspindlin component, MKLP1, is a plus enddirected microtubule motor protein. Centralspindlin bundles microtubules in the spindle midzone, (38) and its components have also been localized to astral microtubules in the region near the equator prior to furrowing.…”

Section: How Are Zones Generated?mentioning

confidence: 99%

Rho GTPase activity zones and transient contractile arrays

2006

View full text Add to dashboard Cite

SummaryThe Rho GTPases-Rho, Rac and Cdc42-act as molecular switches, cycling between an active GTP-bound state and an inactive GDP-bound state, to regulate the actin cytoskeleton. It has recently become apparent that the Rho GTPases can be activated in subcellular zones that appear semi-stable, yet are dynamically maintained. These Rho GTPase activity zones are associated with a variety of fundamental biological processes including symmetric and asymmetric cytokinesis and cellular wound repair. Here we review the basic features of Rho GTPase activity zones, suggest that these zones represent a fundamental signaling mechanism, and discuss the implications of zone properties from the perspective of both their function and how they are likely to be controlled.

show abstract

Central Spindle Assembly and Cytokinesis Require a Kinesin-like Protein/RhoGAP Complex with Microtubule Bundling Activity

Cited by 464 publications

References 32 publications

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Mid1/anillin and the spatial regulation of cytokinesis in fission yeast

Cytokinesis and cancer

Rho GTPase activity zones and transient contractile arrays

Contact Info

Product

Resources

About