Central serotonin attenuates LPS-induced systemic inflammation

Mota, Clarissa M.D.; Rodrigues-Santos, Caroline; Fernández, Raúl; Carolino, Ruither Oliveira Gomes; Antunes‐Rodrigues, José; Anselmo-Franci, Janete Aparecida; Branco, Luiz G.S.

doi:10.1016/j.bbi.2017.07.010

Cited by 25 publications

(10 citation statements)

References 85 publications

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“…Additionally, kynurenic acid, which is detoxified kynurenine, reduced the release of IL-4 through the specific activation of G protein-coupled receptor 35 (GPR35) [34], and quinolinic acid have a role in protect the body from inflammatory injury via a direct action on neutrophils or vascular permeability 20 [35]. Melatonin, serotonin and N-acetylserotonin also have ability to inhibit production of inflammatory cytokines and superoxide [36,37,38]. Meanwhile, increase in the production of glutamic acid (glutamate) and NADP have a favor role involved in the purine metabolism and glutathione metabolism while coenzyme A affect phenylacetate metabolism.…”

Section: Discussionmentioning

confidence: 99%

Piper sarmentosum extract alleviates inflammatory responses and improves barrier function in porcine intestinal epithelial (IPEC-J2) cells induced by lipopolysaccharide (LPS)

Wang¹,

Zhou²,

Hanlin³

et al. 2020

Preprint

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Background: In this study, we investigated the anti-inflammatory effect of Piper sarmentosum extract (PSE) in the IPEC-J2 cells induced by lipopolysaccharide (LPS). Meanwhile, we also tested the metabolomics profile of cells treated by LPS and PSE.Method: IPEC-J2 cells (6×10 5 cell/well) were seeded on 6-well plates, and cells were divided into three treatments (control, LPS treatment and LPS + PSE-NB treatment). Each treatment was conducted in five replicates. After incubation for 24 h, cells in LPS + PSE-NB treatment were treated by media containing PSE-NB at 10 ug/ml (cells in control and LPS treatments were treated by complete medium). Cells were culture for 24 h, and cells in LPS treatment and LPS + PSE-NB treatment then were treated by media contain 1 μg/ml of LPS (cells in control was treated by complete medium) for another 24 h. After treatment, cells were used for gene expression assays, protein expression assays and metabolomics analysis. Results:We demonstrated that LPS stimulation significantly up-regulated the mRNA expression of IL-1, IL-6 and TNF-α (P < 0.05) compared with the control in the IPEC-J2 cells. Piper sarmentosum extract with n-butanol (PSE-NB) pre-treatment with 10 ug/mL before LPS stimulation significantly decreased the expression of IL-1, IL-6 and TNF-α compared with LPS treatment (P < 0.05). We found that PSE-NB improved the expression of intestinal tight junction proteins (ZO1 and Occludin) and NHE3 that were reduced by LPS stimulation (P < 0.05). Moreover, PSE-NB alleviated LPS-induced protein expression of p65 and p-p65 (P < 0.05) and inhibited the NF-κB signaling pathway. Metabolic pathway analysis indicated that PSE-NB exert anti-inflammatory activity mainly through affecting tryptophan metabolism. Its metabolic product, melatonin, has anti-inflammatory properties by inhibition of NF-κB activation, which consistent with our results regarding to anti-inflammatory activity of PSE-NB on inflammatory signaling pathway. Conclusion: These results suggested that PSE-NB might attenuate LPS-induced inflammatory responses in the IPEC-J2 cells by regulating inflammatory NF-κB signaling pathway and intracellular metabolic pattern. Background 3 Intestinal epithelial cells (IECs) play important roles in the animal's physical defense, which is the first line of defense against the invasion of various pathogens and antigens in the external environment of gut lumen [1]. Impaired intestinal epithelium tends to be associated with a series of both intestinal and systemic diseases, including intestinal inflammation and diarrhea [2]. Although gut inflammation may not cause the full-blown clinical symptoms in weaned piglets, it could lead to increased mucosal permeability and reduced growth performance [3]. So, it is necessary to prevent gut inflammation and maintain normal barrier function of IECs in animals. Piper sarmentosum, is a terrestrial herb. This plant is distributed mainly in tropical and subtropical region of Asia. It is used as a folk medicine in certain countries of Southeast Asia for the...

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Section: Discussionmentioning

confidence: 99%

Piper sarmentosum extract alleviates inflammatory responses and improves barrier function in porcine intestinal epithelial (IPEC-J2) cells induced by lipopolysaccharide (LPS)

Wang¹,

Zhou²,

Hanlin³

et al. 2020

Preprint

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“…Martins [78] suggested that plasma LPS should be monitored in early life to prevent mitochondrial apoptosis in neurodegenerative diseases. Mota et al [79] investigated the relationship between central 5-HT and LPS-induced systemic inflammation in animals. In this study, the authors showed a marked decrease in both the Anteroventral Preoptic Region (AVPO) 5-HT and 5-HIAA 300 min after LPS administration and enhanced corticosterone concentrations 45 and 300 min after LPS administration.…”

Section: Gut Motilitymentioning

confidence: 99%

The Relationship Between the Serotonin Metabolism, Gut-Microbiota and the Gut-Brain Axis

Stasi

Sadalla

Milani

2019

CDM

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Background:: Serotonin (5-HT) has a pleiotropic function in gastrointestinal, neurological/psychiatric and liver diseases. The aim of this review was to elucidate whether the gut-microbiota played a critical role in regulating peripheral serotonin levels. Methods:: We searched for relevant studies published in English using the PubMed database from 1993 to the present. Results: : Several studies suggested that alterations in the gut-microbiota may contribute to a modulation of serotonin signalling. The first indication regarded the changes in the composition of the commensal bacteria and the intestinal transit time caused by antibiotic treatment. The second indication regarded the changes in serotonin levels correlated to specific bacteria. The third indication regarded the fact that decreased serotonin transporter expression was associated with a shift in gut-microbiota from homeostasis to inflammatory type microbiota. Serotonin plays a key role in the regulation of visceral pain, secretion, and initiation of the peristaltic reflex; however, its altered levels are also detected in many different psychiatric disorders. Symptoms of some gastrointestinal functional disorders may be due to deregulation in central nervous system activity, dysregulation at the peripheral level (intestine), or a combination of both (brain-gut axis) by means of neuro-endocrine-immune stimuli. Moreover, several studies have demonstrated the profibrogenic role of 5-HT in the liver, showing that it works synergistically with platelet-derived growth factor in stimulating hepatic stellate cell proliferation. Conclusion:: Although the specific interaction mechanisms are still unclear, some studies have suggested that there is a correlation between the gut-microbiota, some gastrointestinal and liver diseases and the serotonin metabolism.

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“…Serotonin (5‐hydroxytryptamine, 5‐HT) is an important neurotransmitter and hormone that modulates many physiological functions including cardiovascular, respiration, immune, energy balance and body temperature . Changes in the level of circulating 5‐HT have the potential to influence the CNS regulation of homeostatic functions either through alterations in afferent signalling or through direct effects on CNS 5‐HT receptors (5‐HTR), although whether 5‐HT crosses the blood‐brain barrier remains controversial .…”

Section: Introductionmentioning

confidence: 99%

Systemic serotonin inhibits brown adipose tissue sympathetic nerve activity via a GABA input to the dorsomedial hypothalamus, not via 5HT_1A receptor activation in raphe pallidus

et al. 2019

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Aim Serotonin (5‐hydroxytryptamine, 5‐HT), an important neurotransmitter and hormone, modulates many physiological functions including body temperature. We investigated neural mechanisms involved in the inhibition of brown adipose tissue (BAT) sympathetic nerve activity (SNA) and BAT thermogenesis evoked by 5‐HT. Methods Electrophysiological recordings, intravenous (iv) injections and nanoinjections in the brains of anaesthetized rats. Results Cooling‐evoked increases in BAT SNA were inhibited by the intra‐rostral raphé pallidus (rRPa) and the iv administration of the 5‐HT1A receptor agonist, 8‐OH‐DPAT or 5‐HT. The intra‐rRPa 5‐HT, the intra‐rRPa and the iv 8‐OH‐DPAT, but not the iv 5‐HT‐induced inhibition of BAT SNA were prevented by nanoinjection of a 5‐HT1A receptor antagonist in the rRPa. The increase in BAT SNA evoked by nanoinjection of NMDA in the rRPa was not inhibited by iv 5‐HT, indicating that iv 5‐HT does not inhibit BAT SNA by acting in the rRPa or in the sympathetic pathway distal to the rRPa. In contrast, under a warm condition, blockade of 5HT1A receptors in the rRPa increased BAT SNA and BAT thermogenesis, suggesting that endogenous 5‐HT in the rRPa contributes to the suppression of BAT SNA and BAT thermogenesis. The increases in BAT SNA and BAT thermogenesis evoked by nanoinjection of NMDA in the dorsomedial hypothalamus (DMH) were inhibited by iv 5‐HT, but those following bicuculline nanoinjection in the DMH were not inhibited. Conclusions The systemic 5‐HT‐induced inhibition of BAT SNA requires a GABAergic inhibition of BAT sympathoexcitatory neurones in the DMH. In addition, during warming, 5‐HT released endogenously in rRPa inhibits BAT SNA.

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Central serotonin attenuates LPS-induced systemic inflammation

Cited by 25 publications

References 85 publications

Piper sarmentosum extract alleviates inflammatory responses and improves barrier function in porcine intestinal epithelial (IPEC-J2) cells induced by lipopolysaccharide (LPS)

Piper sarmentosum extract alleviates inflammatory responses and improves barrier function in porcine intestinal epithelial (IPEC-J2) cells induced by lipopolysaccharide (LPS)

The Relationship Between the Serotonin Metabolism, Gut-Microbiota and the Gut-Brain Axis

Systemic serotonin inhibits brown adipose tissue sympathetic nerve activity via a GABA input to the dorsomedial hypothalamus, not via 5HT_1A receptor activation in raphe pallidus

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Central serotonin attenuates LPS-induced systemic inflammation

Cited by 25 publications

References 85 publications

Piper sarmentosum extract alleviates inflammatory responses and improves barrier function in porcine intestinal epithelial (IPEC-J2) cells induced by lipopolysaccharide (LPS)

Piper sarmentosum extract alleviates inflammatory responses and improves barrier function in porcine intestinal epithelial (IPEC-J2) cells induced by lipopolysaccharide (LPS)

The Relationship Between the Serotonin Metabolism, Gut-Microbiota and the Gut-Brain Axis

Systemic serotonin inhibits brown adipose tissue sympathetic nerve activity via a GABA input to the dorsomedial hypothalamus, not via 5HT1A receptor activation in raphe pallidus

Contact Info

Product

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About

Systemic serotonin inhibits brown adipose tissue sympathetic nerve activity via a GABA input to the dorsomedial hypothalamus, not via 5HT_1A receptor activation in raphe pallidus