Central role of <i>Fa<scp>GAMYB</scp></i> in the transition of the strawberry receptacle from development to ripening

Vallarino, José G.; Osorio, Sonia; Bombarely, Aureliano; Casañal, Ana; Cruz-Rus, Eduardo; Sánchez-Sevilla, José F.; Amaya, Iraida; Giavalisco, Patrick; Fernie, Alisdair R.; Botella, Miguel A.; Valpuesta, Victoriano

doi:10.1111/nph.13463

Cited by 64 publications

(59 citation statements)

References 58 publications

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“…Here, we found that silencing of FaMADS9 causes changes in the expression of an elevated number of ARFs but also a drastic reduction in the expression of ABA‐synthesizing genes such as FaNCED1 , FaNCED2 and FaNCED3 in the transgenic receptacles, and a subsequent decrease in AB A content. Silencing of FaNCED1 and FaNCED2 was observed in FaGAMYB ‐silenced receptacles (Vallarino et al , ), as well as reduced levels of ABA in the transgenic tissue. Notably, the ASR gene ( FvH4_2g13410 ), which is involved in the ABA transduction pathway, was up‐regulated in red receptacles.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the hormones have been reported to participate in strawberry fruit development and ripening (Chai et al , ; Csukasi et al , ; Estrada‐Johnson et al , ; Jia et al , ; Merchante et al , ; Mezzetti et al , ), and they are expected to play a main role in the regulation of the process. However, the interplay between hormones and regulatory genes has scarcely been addressed (Csukasi et al , ; Vallarino et al , ). Here, we found that silencing of FaMADS9 causes changes in the expression of an elevated number of ARFs but also a drastic reduction in the expression of ABA‐synthesizing genes such as FaNCED1 , FaNCED2 and FaNCED3 in the transgenic receptacles, and a subsequent decrease in AB A content.…”

Section: Discussionmentioning

confidence: 99%

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Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Vallarino

Merchante

Sánchez-Sevilla

et al. 2019

Plant Biotechnology Journal

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FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines did not show differences in basic parameters, such as fruit firmness or colour, but exhibited lower Brix values in three of the four independent lines. The gene ontology MapMan category that was most enriched among the differentially expressed genes in the receptacles at the white stage corresponded to the regulation of transcription, including a high percentage of transcription factors and regulatory proteins associated with auxin action. In contrast, the most enriched categories at the red stage were transport, lipid metabolism and cell wall. Metabolomic analysis of the receptacles of the transformed fruits identified significant changes in the content of maltose, galactonic acid‐1,4‐lactone, proanthocyanidins and flavonols at the green/white stage, while isomaltose, anthocyanins and cuticular wax metabolism were the most affected at the red stage. Among the regulatory genes that were differentially expressed in the transgenic receptacles were several genes previously linked to flavonoid metabolism, such as MYB10, DIV, ZFN1, ZFN2, GT2, and GT5, or associated with the action of hormones, such as abscisic acid, SHP, ASR, GTE7 and SnRK2.7. The inference of a gene regulatory network, based on a dynamic Bayesian approach, among the genes differentially expressed in the transgenic receptacles at the white and red stages, identified the genes KAN1, DIV, ZFN2 and GTE7 as putative targets of FaMADS9. A MADS9‐specific CArG box was identified in the promoters of these genes.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Vallarino

Merchante

Sánchez-Sevilla

et al. 2019

Plant Biotechnology Journal

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“…The bHLH proteins have not been extensively studied in plants. Those that have been characterized function in anthocyanin biosynthesis, phytochrome signaling, globulin expression, fruit dehiscence, and carpel and epidermal development5253. Consequently, gene27422 could encode a bHLH TF regulating pigment formation in strawberry fruit.…”

Section: Discussionmentioning

confidence: 99%

Early metabolic and transcriptional variations in fruit of natural white-fruited Fragaria vesca genotypes

Härtl

Denton

Franz-Oberdorf

et al. 2017

Sci Rep

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Strawberry fruits (Fragaria vesca) are valued for their sweet fruity flavor, juicy texture, and characteristic red color caused by anthocyanin pigments. To gain a deeper insight into the regulation of anthocyanin biosynthesis, we performed comparative metabolite profiling and transcriptome analyses of one red-fruited and two natural white-fruited strawberry varieties in two tissues and three ripening stages. Developing fruit of the three genotypes showed a distinctive pattern of polyphenol accumulation already in green receptacle and achenes. Global analysis of the transcriptomes revealed that the ripening process in the white-fruited varieties is already affected at an early developmental stage. Key polyphenol genes showed considerably lower transcript levels in the receptacle and achenes of both white genotypes, compared to the red genotype. The expression of the anthocyanidin glucosyltransferase gene and a glutathione S-transferase, putatively involved in the vacuolar transport of the anthocyanins, seemed to be critical for anthocyanin formation. A bHLH transcription factor is among the differentially expressed genes as well. Furthermore, genes associated with flavor formation and fruit softening appear to be coordinately regulated and seem to interact with the polyphenol biosynthesis pathway. This study provides new information about polyphenol biosynthesis regulators in strawberry, and reveals genes unknown to affect anthocyanin formation.

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“…Accordingly, we were able to apply the protocol for the extraction, detection and identification of primary and/or secondary metabolites of several species and tissues. Amongst others, we studied Arabidopsis thaliana seedlings [32], roots and leaves [23], barley ( Hordeum vulgare ) [62], wild strawberry ( Fragaria vesca ) [63] strawberry ( Fragaria X ananassa ) [64], the root tissue of maize ( Zea mays ) [65], the green algae Chlamydomonas reinhardtii [66], the marine diatom Thalassiosira pseudonana [61] and some low phosphate-tolerant proteaceae species [51]. …”

Section: Discussionmentioning

confidence: 99%

Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

et al. 2016

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BackgroundThe elucidation of complex biological systems requires integration of multiple molecular parameters. Accordingly, high throughput methods like transcriptomics, proteomics, metabolomics and lipidomics have emerged to provide the tools for successful system-wide investigations. Unfortunately, optimized analysis of different compounds requires specific extraction procedures in combination with specific analytical instrumentation. However, the most efficient extraction protocols often only cover a restricted number of compounds due to the different physico-chemical properties of these biological compounds. Consequently, comprehensive analysis of several molecular components like polar primary metabolites next to lipids or proteins require multiple aliquots to enable the specific extraction procedures required to cover these diverse compound classes. This multi-parallel sample handling of different sample aliquots is therefore not only more sample intensive, it also requires more time and effort to obtain the required extracts.ResultsTo circumvent large sample amounts, distributed into several aliquots for the comprehensive extraction of most relevant biological compounds, we developed a simple, robust and reproducible two-phase liquid–liquid extraction protocol. This one-step extraction protocol allows for the analysis of polar-, semi-polar and hydrophobic metabolites, next to insoluble or precipitated compounds, including proteins, starch and plant cell wall components, from a single sample. The method is scalable regarding the used sample amounts but also the employed volumes and can be performed in microcentrifuge tubes, enabling high throughput analysis. The obtained fractions are fully compatible with common analytical methods, including spectroscopic, chromatographic and mass spectrometry-based techniques. To document the utility of the described protocol, we used 25 mg of Arabidopsis thaliana rosette leaves for the generation of multi-omics data sets, covering lipidomics, metabolomics and proteomics. The obtained data allowed us to measure and annotate more than 200 lipid compounds, 100 primary metabolites, 50 secondary metabolites and 2000 proteins.ConclusionsThe described extraction protocol provides a simple and straightforward method for the efficient extraction of lipids, metabolites and proteins from minute amounts of a single sample, enabling the targeted but also untargeted high-throughput analyses of diverse biological tissues and samples.Electronic supplementary materialThe online version of this article (doi:10.1186/s13007-016-0146-2) contains supplementary material, which is available to authorized users.

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Central role of FaGAMYB in the transition of the strawberry receptacle from development to ripening

Cited by 64 publications

References 58 publications

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Early metabolic and transcriptional variations in fruit of natural white-fruited Fragaria vesca genotypes

Protocol: a fast, comprehensive and reproducible one-step extraction method for the rapid preparation of polar and semi-polar metabolites, lipids, proteins, starch and cell wall polymers from a single sample

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