Central role of 70-kDa heat shock protein in adaptation of plants to drought stress

Aghaie, Peyman; Tafreshi, Seyed Ali Hosseini

doi:10.1007/s12192-020-01144-7

Cited by 46 publications

(20 citation statements)

References 59 publications

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“…Furthermore, most of the DEGs encoding HSPs were up-regulated in the roots of P. ussuriensis under PEG-induced drought stress. The result is consistent with previous studies on Capsicum annuum , O. sativa , and Solanum lycopersicum showing that HSPs positively regulate the tolerance to drought stress [ 11 , 15 , 59 ].…”

Section: Discussionsupporting

confidence: 93%

Characterization of the Gene Expression Profile Response to Drought Stress in Populus ussuriensis Using PacBio SMRT and Illumina Sequencing

Liu

et al. 2022

IJMS

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In this study, we characterized the gene expression profile in the roots of Populus ussuriensis at 0, 6, 12, 24, 48 and 120 h after the start of polyethylene glycol (PEG)-induced drought stress using PacBio single-molecule real-time sequencing (SMRT-seq) and Illumina RNA sequencing. Compared to the control, 2244 differentially expressed genes (DEGs) were identified, and many of these DEGs were associated with the signal transduction, antioxidant system, ion accumulation and drought-inducing proteins. Changes in certain physiological and biochemical indexes, such as antioxidant activity and the contents of Ca2+, proline, and total soluble sugars, were further confirmed in P. ussuriensis roots. Furthermore, most of the differentially expressed transcription factors were members of the AP2/ERF, C2H2, MYB, NAC, C2C2 and WRKY families. Additionally, based on PacBio SMRT-seq results, 5955 long non-coding RNAs and 700 alternative splicing events were identified. Our results provide a global view of the gene expression profile that contributes to drought resistance in P. ussuriensis and meaningful information for genetic engineering research in the future.

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Section: Discussionsupporting

confidence: 93%

Characterization of the Gene Expression Profile Response to Drought Stress in Populus ussuriensis Using PacBio SMRT and Illumina Sequencing

Liu

et al. 2022

IJMS

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“…In addition, in B+ Myc roots there was the accumulation of two plastid heat shock proteins (Lj6g3v0704480, Lj1g3v4865370), which play a critical role in maintaining the cellular homeostasis under normal plant life or during stress conditions (Aghaie and Tafreshi, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Symbiotic responses of Lotus japonicus to two isogenic lines of a mycorrhizal fungus differing in the presence/absence of an endobacterium

et al. 2021

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Summary As other arbuscular mycorrhizal fungi, Gigaspora margarita contains unculturable endobacteria in its cytoplasm. A cured fungal line has been obtained and showed it was capable of establishing a successful mycorrhizal colonization. However, previous OMICs and physiological analyses have demonstrated that the cured fungus is impaired in some functions during the pre‐symbiotic phase, leading to a lower respiration activity, lower ATP, and antioxidant production. Here, by combining deep dual‐mRNA sequencing and proteomics applied to Lotus japonicus roots colonized by the fungal line with bacteria (B+) and by the cured line (B−), we tested the hypothesis that L. japonicus (i) activates its symbiotic pathways irrespective of the presence or absence of the endobacterium, but (ii) perceives the two fungal lines as different physiological entities. Morphological observations confirmed the absence of clear endobacteria‐dependent changes in the mycorrhizal phenotype of L. japonicus, while transcript and proteomic datasets revealed activation of the most important symbiotic pathways. They included the iconic nutrient transport and some less‐investigated pathways, such as phenylpropanoid biosynthesis. However, significant differences between the mycorrhizal B+/B− plants emerged in the respiratory pathways and lipid biosynthesis. In both cases, the roots colonized by the cured line revealed a reduced capacity to activate genes involved in antioxidant metabolism, as well as the early biosynthetic steps of the symbiotic lipids, which are directed towards the fungus. Similar to its pre‐symbiotic phase, the intraradical fungus revealed transcripts related to mitochondrial activity, which were downregulated in the cured line, as well as perturbation in lipid biosynthesis.

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“…This phenomenon is called acquired tolerance [38,39]. The heat stress response is characterised by an elevated synthesis of a specific set of heat shock proteins (Hsps) associated with the development of thermotolerance [40][41][42]. Among the genes involved in the heat stress response, we observed the gene encoding for Hsp70 to be positively regulated by heat stress (Table 2).…”

Section: Heat Stress Modelmentioning

confidence: 96%

Meta-Analysis as a Tool to Identify Candidate Genes Involved in the Fagus sylvatica L. Abiotic Stress Response

Garosi

Ferrante

Vettori

et al. 2022

Forests

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In this study, we aimed to evaluate whether candidate genes for abiotic stresses in Fagus sylvatica L. are also candidate genes for herbaceous plants, with the purpose of better defining the abiotic stress response model of F. sylvatica. Therefore, a meta-analysis was performed on published papers related to abiotic stress. Firstly, we carried out a systematic review regarding the activity of 24 candidate genes selected for F. sylvatica under abiotic stress reported in 503 articles. After choosing the inclusion criteria, 73 articles out of 503, regarding 12 candidate genes, were included in this analysis. We performed an exploratory meta-analysis based on the random-effect model and the combined effect-size approach (Cohen’s d). The results obtained through Forest and Funnel plots indicate that the candidate genes for F. sylvatica are considered to be candidate genes in other herbaceous species. These results allowed us to set up models of plants’ response to abiotic stresses implementing the stress models in forest species. The results of this study will serve to bridge knowledge gaps regarding the pathways of response to abiotic stresses in trees based on the meta-analysis. The study approach used could be extended to observe larger gene databases and different species.

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Central role of 70-kDa heat shock protein in adaptation of plants to drought stress

Cited by 46 publications

References 59 publications

Characterization of the Gene Expression Profile Response to Drought Stress in Populus ussuriensis Using PacBio SMRT and Illumina Sequencing

Characterization of the Gene Expression Profile Response to Drought Stress in Populus ussuriensis Using PacBio SMRT and Illumina Sequencing

Symbiotic responses of Lotus japonicus to two isogenic lines of a mycorrhizal fungus differing in the presence/absence of an endobacterium

Meta-Analysis as a Tool to Identify Candidate Genes Involved in the Fagus sylvatica L. Abiotic Stress Response

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