Central Insulin Administration Maintains Reproductive Behavior in Diabetic Female Rats

Kovács, Péter; Morales, José Carlos Roldán; Karkanias, George

doi:10.1159/000071964

Cited by 26 publications

(7 citation statements)

References 32 publications

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“…Lower lordosis quotients in sexual behavior tests have also been reported [18,19]. Estrous cycle duration and sexual behavior were not impaired in the STZ rats in the present study.…”

Section: Discussionsupporting

confidence: 82%

Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats

Kiss

Woodside

Sinzato

et al. 2013

Diabetol Metab Syndr

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BackgroundNeonatal STZ treatment induces a state of mild hyperglycemia in adult rats that disrupts metabolism and maternal/fetal interactions. The aim of this study was investigate the effect of neonatal STZ treatment on the physical development, behavior, and reproductive function of female Wistar rats from infancy to adulthood.MethodsAt birth, litters were assigned either to a Control (subcutaneous (s.c.) citrate buffer, n = 10) or STZ group, (streptozotocin (STZ) - 100 mg/kg-sc, n = 6). Blood glucose levels were measured on postnatal days (PND) 35, 84 and 120. In Experiment 1 body weight, length and the appearance of developmental milestones such as eye and vaginal opening were monitored. To assess the relative contribution of the initial and long term effects of STZ treatment this group was subdivided based on blood glucose levels recorded on PND 120: STZ hyperglycemic (between 120 and 300 mg/dl) and STZ normoglycemic (under 120 mg/dl). Behavioral activity was assessed in an open field on PND 21 and 75. In Experiment 2 estrous cyclicity, sexual behavior and circulating gonadotropin, ovarian steroid, and insulin levels were compared between control and STZ-hyperglycemic rats. In all measures the litter was the experimental unit. Parametric data were analyzed using one-way or, where appropriate, two-way ANOVA and significant effects were investigated using Tukey’s post hoc test. Fisher’s exact test was employed when data did not satisfy the assumption of normality e.g. presence of urine and fecal boli on the open field between groups. Statistical significance was set at p < 0.05 for all data.ResultsAs expected neonatal STZ treatment caused hyperglycemia and hypoinsulinemia in adulthood. STZ-treated pups also showed a temporary reduction in growth rate that probably reflected the early loss of circulating insulin. Hyperglycemic rats also exhibited a reduction in locomotor and exploratory behavior in the open field. Mild hyperglycemia did not impair gonadotropin levels or estrous cylicity but ovarian steroid concentrations were altered.ConclusionsIn female Wistar rats, neonatal STZ treatment impairs growth in infancy and results in mild hyperglycemia/hypoinsulinemia in adulthood that is associated with changes in the response to a novel environment and altered ovarian steroid hormone levels.

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“…Lower lordosis quotients in sexual behavior tests have also been reported [18,19]. Estrous cycle duration and sexual behavior were not impaired in the STZ rats in the present study.…”

Section: Discussionsupporting

confidence: 82%

Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats

Kiss

Woodside

Sinzato

et al. 2013

Diabetol Metab Syndr

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“…Alternatively, it is clear that insulin signaling has functions in the CNS not related to glucose metabolism. For example, insulin administered to the third ventricle at doses insufficient to reduce hyperglycemia rescues sexual behavior in STZ-induced diabetic rats and somewhat enhances that behavior in normal controls (Kovacs et al, 2003). Moreover, insulin receptor knockout mice show increased phosphorylation of the microtubule-associated protein Tau but no alteration in basal brain glucose metabolism (Schubert et al., 2004).…”

Section: Discussionmentioning

confidence: 99%

Changes in mitotic rate and GFAP expression in the primary olfactory axis of Streptozotocin-induced diabetic rats

et al. 2005

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Many diabetic individuals develop anosmia but the mechanism(s) causing the dysfunction in the olfactory system is (are) unknown. Glial fibrillary acidic protein expression is reduced in diabetic retinopathy and is also reduced, with unknown consequences, in other brain regions of diabetic rats. We used immunohistochemistry and immunoblotting from untreated control and streptozotocin-induced type 1 (insulin dependent) diabetic rats to investigate main olfactory epithelial mitotic rate and glial fibrillary acidic protein expression in the lamina propria of the sensory epithelium and in the olfactory bulb. Numbers of bromodeoxyuridine-positive cells were significantly lower in the diabetic sensory epithelium compared to non-diabetic controls. Immunohistochemical observations suggested a qualitative difference in glial fibrillary acidic protein expression in both regions examined especially in the olfactory bulb external plexiform layer and the lamina propria. Immunoblot analysis confirmed that the diabetic olfactory bulb and lamina propria expressed less glial fibrillary acidic protein compared to the non-diabetic control group. The lower expression levels in the olfactory bulb external plexiform layer suggested by immunohistochemistry do not reflect a change in the number of astrocytes since the numbers of S100B(+) cells were not different between the two groups.

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“…The original purpose of this study was to determine if TA may restore reproductive function in diabetic rats that always suffer with the low reproductive capability [1,10]. We …”

Section: Discussionmentioning

confidence: 99%

“…TA can depress high plasma glucose level in diabetic mouse models of insulin dependent diabetic mellitus (IDDM) and non-insulin dependent diabetic mellitus (NIDDM) [7,8]. It is well known that diabetic patients usually suffer from sexual dysfunction problems [1,10]. TA, one of the Tremalleceae family, has medical effects on strengthening sexual function [3].…”

Section: Introductionmentioning

confidence: 99%

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THE EFFECTS OFTREMELLA AURANTIAON TESTOSTERONE AND CORTICOSTERONE PRODUCTIONS IN NORMAL AND DIABETIC RATS

Lo¹,

Yang

Liu

et al. 2004

Archives of Andrology

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Tremella aurantia (TA) has been traditionally used as food and crude medicine in Chinese society. The polysaccharide isolated from the fruiting bodies of TA exhibits significant hypoglycemic activity in diabetic mouse models of insulin-dependent diabetes mellitus (IDDM) and non-insulindependent diabetes mellitus (NIDDM). Diabetes will cause sexual dysfunction in patients. In the present study, we examined if the treatment of TA on IDDM and NIDDM rats will restore steroidogenesis and then the reproductive function. The fruiting bodies (FB), mycelium (TM) and polysaccharide (GX) of TA were fed to the IDDM and NIDDM rats, and testosterone and corticosterone levels in plasma, the weight of steroidogenic organs, and the expression of steroidogenic acute regulatory (StAR) protein and P450scc enzyme were determined. Plasma testosterone productions were significantly suppressed with the feeding of FB or TM in normal rat (p < 0.05). Testosterone productions were also significantly suppressed in IDDM diabetes rats (p < 0.05), and FB or TM could not restore the inhibitory effects (p > 0.05). There was no significant difference of the testosterone production between normal and NIDDM rats (p > 0.05). In plasma corticosterone production, there were no differences among control, FB-or TM-fed normal rats (p > 0.05). Corticosterone levels were reduced in IDDM rats compared to control, and FB or TM could restore its level. Corticosterone levels were induced in NIDDM rats compared to control (p < 0.05), but FB, TM or GX significantly brought the corticosterone back (p < 0.05) to the control levels. Considering steroidogenic organs, IDDM rats with or without TA treatments had heavier testis and adrenal glands, but not epididymis, than normal rats with or without TA TA is kindly supplied by Dr. Solomon P. Wasser (Institute of Evolution, Haifa University, Mt Carmel 31905, Haifa, Israel). This work was supported by National Science Council Grants NSC 92-2320-B006-054 to BMH, Taiwan.Address correspondence to Bu-Miin Huang, Ph.D., Department of Cell Biology and Anatomy, College of Medicine, National Cheng Kung University, Tainan, Taiwan, Republic of China. E-mail: bumiin@mail.ncku. edu.tw 395 treatments. There were no effects of TA on the weight of steroidogenic organs among normal and NIDDM rats. However, GX feeding in NIDDM rat had lesser testis weight compared to NIDDM rats. The expression of StAR protein and P450scc enzyme were not different among groups in IDDM and NIDDM rats. Plasma testosterone productions were suppressed in normal rats with the feeding of TA (FB and TM). IDDM rats did have lower testosterone, but not in NIDDM, and FB or TM could not restore the inhibitory effects. The induction of IDDM or NIDDM rats did affect steroidogenesis and steroidogenic organ weights, and the feeding of TA had different effects on steroidogenesis in different types of diabetic rats.

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Central Insulin Administration Maintains Reproductive Behavior in Diabetic Female Rats

Cited by 26 publications

References 32 publications

Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats

Neonatally induced mild diabetes: influence on development, behavior and reproductive function of female Wistar rats

Changes in mitotic rate and GFAP expression in the primary olfactory axis of Streptozotocin-induced diabetic rats

THE EFFECTS OFTREMELLA AURANTIAON TESTOSTERONE AND CORTICOSTERONE PRODUCTIONS IN NORMAL AND DIABETIC RATS

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