Central GABAA receptor mediates taurine-induced hypothermia and possibly reduces food intake in thermo-neutral chicks and regulates plasma metabolites in heat-exposed chicks

Elhussiny, Mohamed Z.; Tran, Phuong V.; Pham, Cuong; Nguyen, Linh T.; Haraguchi, Shogo; Gilbert, Elizabeth R.; Cline, Mark A.; Bungo, Takashi; Furuse, Mitsuhiro; Chowdhury, Vishwajit S.

doi:10.1016/j.jtherbio.2021.102905

Cited by 10 publications

(9 citation statements)

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“…CRF induces hyperthermia through the activation of the HPA axis [41] and the dose-dependently increased O 2 consumption, CO 2 production and heat production in the chicks [42]. On the other hand, the central injection of taurine induced dose-dependent hypothermia in chicks [32] and in rabbits [43]. It could be speculated that taurine-induced hypothermia and attenuated CRF magnified SIH through a reduction in heat production.…”

Section: Discussionmentioning

confidence: 99%

“…The taurine and CRF were dissolved in 0.85% saline containing 0.1% Evans blue. Evans blue solution was injected into the control group chicks, as described [32,63]. The drugs were incubated on ice during the experiments.…”

Section: Injection Proceduresmentioning

confidence: 99%

“…On the day of the experiments, the chicks (five days old; n = 10) were injected with 10 µL of saline, CRF (2.1 pmol [6,16]), taurine (2.5 µmol [32]), or CRF plus taurine (2.1 pmol + 2.5 µmol). The chicks were then individually housed in an acrylic glass chamber (40 cm × 30 cm × 20 cm) at a temperature of 30 ± 1 • C, and water and diet were withheld.…”

Section: Experimental Designmentioning

confidence: 99%

“…The vocalizations were counted using a digital hand tally counter, and the behaviors were analyzed by a researcher blind to treatment. A digital thermometer with a precision of ±0.1 • C (Thermalert TH-5, Physitemp Instruments Inc., Clifton, NJ, USA) was used to measure the rectal temperature at 0 and 10 min post-injection by inserting the thermistor probe into the rectum via the cloaca to a depth of approximately 2 cm, as we reported [32,67]. The chicks were anesthetized 10 min post-ICV injection with isoflurane (Mylan Pharmaceutical Co., Ltd., Tokyo, Japan) before the collection of blood samples from the jugular vein.…”

Section: Experimental Designmentioning

confidence: 99%

“…Taurine, an inhibitory neurotransmitter in the brain, has a positive allosteric modulatory effect on ligand-gated chloride channels in neurons, including the ionotropic γ-amino butyric acid receptor (GABA A -R) and the glycine receptor, as well as inhibitory effects on other ligand-and voltage-gated cation channels, such as the N-methyl-D-aspartate receptor [30,31]. Our recent findings showed that the central injection of taurine caused hypothermia in a dose-dependent manner in chicks that was mediated through GABA A -R [32]. In addition, taurine was reported to induce anxiolytic effects in mice and rats [33,34], and the consumption of taurine-supplemented diets was associated with antidepressant-like behaviors in forced swimming-tested mice [35].…”

Section: Introductionmentioning

confidence: 99%

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Central Taurine Attenuates Hyperthermia and Isolation Stress Behaviors Augmented by Corticotropin-Releasing Factor with Modifying Brain Amino Acid Metabolism in Neonatal Chicks

et al. 2022

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The objective of this study was to determine the effects of centrally administered taurine on rectal temperature, behavioral responses and brain amino acid metabolism under isolation stress and the presence of co-injected corticotropin-releasing factor (CRF). Neonatal chicks were centrally injected with saline, 2.1 pmol of CRF, 2.5 μmol of taurine or both taurine and CRF. The results showed that CRF-induced hyperthermia was attenuated by co-injection with taurine. Taurine, alone or with CRF, significantly decreased the number of distress vocalizations and the time spent in active wakefulness, as well as increased the time spent in the sleeping posture, compared with the saline- and CRF-injected chicks. An amino acid chromatographic analysis revealed that diencephalic leucine, isoleucine, tyrosine, glutamate, asparagine, alanine, β-alanine, cystathionine and 3-methylhistidine were decreased in response to taurine alone or in combination with CRF. Central taurine, alone and when co-administered with CRF, decreased isoleucine, phenylalanine, tyrosine and cysteine, but increased glycine concentrations in the brainstem, compared with saline and CRF groups. The results collectively indicate that central taurine attenuated CRF-induced hyperthermia and stress behaviors in neonatal chicks, and the mechanism likely involves the repartitioning of amino acids to different metabolic pathways. In particular, brain leucine, isoleucine, cysteine, glutamate and glycine may be mobilized to cope with acute stressors.

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Section: Discussionmentioning

confidence: 99%

Section: Injection Proceduresmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Experimental Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Central Taurine Attenuates Hyperthermia and Isolation Stress Behaviors Augmented by Corticotropin-Releasing Factor with Modifying Brain Amino Acid Metabolism in Neonatal Chicks

et al. 2022

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The effect of 8-day oral taurine supplementation on thermoregulation during low-intensity exercise at fixed heat production in hot conditions of incremental humidity

Peel,

McNarry,

Heffernan

et al. 2024

Eur J Appl Physiol

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Purpose To determine the effect of taurine supplementation on sweating and core temperature responses, including the transition from compensable to uncompensable heat stress, during prolonged low-intensity exercise of a fixed-heat production (~ 200W/m2) in hot conditions (37.5 °C), at both fixed and incremental vapour-pressure. Methods Fifteen females (n = 3) and males (n = 12; 27 ± 5 years, 78 ± 9 kg, $$\dot{V}$$ V ˙ O2max 50.3 ± 7.8 mL/kg/min), completed a treadmill walking protocol (~ 200W/m2 heat production [Ḣprod]) in the heat (37.5 ± 0.1 °C) at fixed-(16-mmHg) and ramped-humidity (∆1.5-mmHg/5-min) following 1 week of oral taurine supplementation (50 mg/kg/bm) or placebo, in a double-blind, randomised, cross-over design. Participants were assessed for whole-body sweat loss (WBSL), local sweat rate (LSR), sweat gland activation (SGA), core temperature (Tcore), breakpoint of compensability (Pcrit) and calorimetric heat transfer components. Plasma volume and plasma taurine concentrations were established through pre- and post-trial blood samples. Results Taurine supplementation increased WBSL by 26.6% and 5.1% (p = 0.035), LSR by 15.5% and 7.8% (p = 0.013), SGA (1 × 1 cm) by 32.2% and 29.9% (p < 0.001) and SGA (3 × 3 cm) by 22.1% and 17.1% (p = 0.015) during the fixed- and ramped-humidity exercise periods, respectively. Evaporative heat loss was enhanced by 27% (p = 0.010), heat-storage reduced by 72% (p = 0.024) and Pcrit was greater in taurine vs placebo (25.0-mmHg vs 21.7-mmHg; p = 0.002). Conclusion Taurine supplementation increased sweating responses during fixed Ḣprod in hot conditions, prior to substantial heat strain and before the breakpoint of compensability, demonstrating improved thermoregulatory capacity. The enhanced evaporative cooling and reduced heat-storage delayed the subsequent upward inflection in Tcore—represented by a greater Pcrit—and offers a potential dietary supplementation strategy to support thermoregulation.

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Intracerebroventricular injection taurine changes free amino acid concentrations in the brain and plasma in chicks

et al. 2022

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Central GABAA receptor mediates taurine-induced hypothermia and possibly reduces food intake in thermo-neutral chicks and regulates plasma metabolites in heat-exposed chicks

Cited by 10 publications

References 46 publications

Central Taurine Attenuates Hyperthermia and Isolation Stress Behaviors Augmented by Corticotropin-Releasing Factor with Modifying Brain Amino Acid Metabolism in Neonatal Chicks

Central Taurine Attenuates Hyperthermia and Isolation Stress Behaviors Augmented by Corticotropin-Releasing Factor with Modifying Brain Amino Acid Metabolism in Neonatal Chicks

The effect of 8-day oral taurine supplementation on thermoregulation during low-intensity exercise at fixed heat production in hot conditions of incremental humidity

Intracerebroventricular injection taurine changes free amino acid concentrations in the brain and plasma in chicks

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