Central composite design in HPMC granulation and correlations between product properties and process parameters

Simone, Veronica De; Dalmoro, Annalisa; Lamberti, Gaetano; D’Amore, Matteo; Barba, Anna Angela

doi:10.1039/c7nj01280b

Cited by 10 publications

(7 citation statements)

References 30 publications

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“…albcians biofilm formation. The physical parameters were analysed by 3 ( k − p ) factorial CCD, minimizing the number of experiments (Kumari & Sarkar, 2014; Simone et al, 2017). The CCD model predicted critical values which were experimentally tested and validated.…”

Section: Discussionmentioning

confidence: 99%

“…Statistical and computational modelling with CCD-based RSM was used to develop a simple, rapid, economic highthroughput method which can establish a relationship between multiple variables for optimization of growth conditions for C. albcians biofilm formation. The physical parameters were analysed by 3 (k − p) factorial CCD, minimizing the number of experiments (Kumari & Sarkar, 2014;Simone et al, 2017). The CCD model predicted critical values which were experimentally tested and validated.…”

Section: Discussionmentioning

confidence: 99%

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Candida albicans biofilm formation and growth optimization for functional studies using response surface methodology

Sadanandan

Vijayalakshmi

Lokesh

et al. 2022

Journal of Applied Microbiology

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Aim: Optimization of Candida albicans growth and biofilm formation is essential for understanding the recalcitrance of this pathogen to advance functional analysis on hospital tools and material surfaces. Optimization and quantification of biofilm have always been a challenge using the conventional one variable at a time (OVAT) method. The present study uses central composite design-based response surface methodology for optimization of conditions to induce growth and biofilm formation in Candida albicans on polystyrene microtiter plates. Methods and Results:Statistical software package, Stat Soft®, STASTICA version 12.6 was used for data analysis. The variables considered in the design matrix were media pH, temperature, incubation period, shaker speed and inoculum size. A fourpronged quantification approach with XTT assay (cell viability), crystal violet assay (biofilm), calcofluor white assay and wet/dry weight measurements (cell mass) was used to understand different aspects of biofilm formation. Cell viability and cell mass were inversely related; however, biofilm was independent of these two factors. The study also highlighted the fact that foetal bovine serum does not significantly contribute to cell adhesion and in turn in vitro biofilm formation in some of the cultures. Conclusions: A high-throughput optimization of C. albicans growth and biofilm formation on polystyrene microplate has been developed and validated. Significance and Impact of Study:This is a first time approach to optimize the interaction of parameters for C. albicans biofilm formation using RSM. Heterogeneity in growth conditions for local strains of C. albicans clinical isolates was observed. This microtiter plate-based method can be used for future screening of therapeutics for the control of C. albicans.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Candida albicans biofilm formation and growth optimization for functional studies using response surface methodology

Sadanandan

Vijayalakshmi

Lokesh

et al. 2022

Journal of Applied Microbiology

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“…The impact that the aforementioned process parameters have on final granule properties is continuously studied to predict the final product quality. A possible approach to study the wet granulation process was that of a recent study conducted by [44], devoted to first planning experiments by the design of experiments (DoEs) and then to use the results to give correlations between product properties and process parameters. In particular, the central composite design (CCD) statistical protocol was applied for planning the experimental campaign about the production of hydroxypropyl methylcellulose (HPMC 20, Pentachem Srl, San Clemente RN-Italy) granules with distilled water as the binder phase and using a bench scale low-shear granulator apparatus.…”

Section: Inside Wet Granulation Runs: Experimental Evidencesmentioning

confidence: 99%

“…It is important to note that several studies in the literature describe the correlations between process parameters and granule properties; however, they use different apparatuses and final products (tablets). It was the first time that, for such similar granulation systems, semiempirical correlations were able to give the combined effect of impeller rotation speed, binder volume, and binder flow rate on granulation yield and flowability [44].…”

Section: Inside Wet Granulation Runs: Experimental Evidencesmentioning

confidence: 99%

“…• Empirical models: they are obtained from the regression of large set of experimental data, most of the time derived from the design of experiments statistic technique [44,48,49]. Empirical models have the advantages of being very simple (often polynomial equations), easy to obtain (regression of data), quite reliable within the investigated range.…”

Section: Mathematical Approach To Describe the Phenomena Involved In mentioning

confidence: 99%

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Inside the Phenomenological Aspects of Wet Granulation: Role of Process Parameters

Simone¹,

Caccavo²,

Dalmoro³

et al. 2018

Granularity in Materials Science

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Granulation is a size-enlargement process by which small particles are bonded, by means of various techniques, in coherent and stable masses (granules), in which the original particles are still identifiable. In wet granulation processes, the powder particles are aggregated through the use of a liquid phase called binder. The main purposes of sizeenlargement process of a powder or mixture of powders are to improve technological properties and/or to realize suitable forms of commercial products. A modern and rational approach in the production of granular structures with tailored features (in terms of size and size distribution, flowability, mechanical and release properties, etc.) requires a deep understanding of phenomena involved during granules formation. By this knowledge, suitable predictive tools can be developed with the aim to choose right process conditions to be used in developing new formulations by avoiding or reducing costs for new tests. In this chapter, after introductive notes on granulation process, the phenomenological aspects involved in the formation of the granules with respect to the main process parameters are presented by experimental demonstration. Possible mathematical approaches in the granulation process description are also presented and the one involving the population mass balances equations is detailed.

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A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

Ashrit

Sadanandan

Natraj

et al. 2022

Polymers for Advanced Techs

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Biofilm formation is one of the crucial factors for virulence in Candida albicans, Escherichia coli and their polymicrobial biofilm formed on medical prosthetics. In this study, high biofilm forming C. albicans M207 from a case of invasive candidiasis and a standard strain of bacterial culture E. coli ATCC 39936 were used for initial screening and media optimization using One Variable at a time (OVAT) and Response Surface Methodology (RSM) models, respectively, in polystyrene microtiter plates for in vitro biofilm studies. Sucrose and peptone significantly affected biofilm formation when screened by OVAT. Media optimization by Central Composite Design (CCD) showed that pH significantly affected biofilm formation for all the cultures which was further validated at critical parameters. Biofilm was quantified by growth OD measurements, MTT and CV assays in the presence and absence of FBS precoating and compared with standard Trypticase Soy Broth (TSB) media which did not show any significant difference. This high throughput polystyrene microtiter plate assay for preliminary screening of nutritional components using OVAT and media optimization using RSM model is being reported for the first time for biofilm formation by C. albicans and E. coli in a single and dual species environment. This study is essential for functional analysis of in‐vitro growth and biofilm formation by these two pathogenic strains on polymeric hospital equipments and to develop suitable control measures to inhibit biofilm formation by C. albicans and E. coli single species and their co‐culture.

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Central composite design in HPMC granulation and correlations between product properties and process parameters

Abstract: Combined effects of rpm, binder volume and binder flow rate on granulation yield and flowability properties have been investigated.

Cited by 10 publications

References 30 publications

Candida albicans biofilm formation and growth optimization for functional studies using response surface methodology

Candida albicans biofilm formation and growth optimization for functional studies using response surface methodology

Inside the Phenomenological Aspects of Wet Granulation: Role of Process Parameters

A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

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