Central and Peripheral Regulation of Appetite and Food Intake in Drosophila

Branch, Audrey; Shen, Ping

doi:10.1201/9781315120171-2

Cited by 9 publications

(6 citation statements)

References 1 publication

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“…We sought to determine whether bam is involved in impacting food consumption of Drosophila , since previous evidence has shown that increased nutrient absorption and storage might be a consequence of elevated intake of foods ( Kohyama-Koganeya, Kurosawa & Hirabayashi, 2015 ; Branch & Shen, 2017 ). To do this, we first performed the cafe assay, a widely used method for directly and accurately measuring feeding rates by using capillary feeds containing liquid media ( Ja et al, 2007 ).…”

Section: Resultsmentioning

confidence: 99%

A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila

Wang

Zhu

Zhang

et al. 2022

PeerJ

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Background Previous studies have proven that bag-of-marbles (bam) plays a pivotal role in promoting early germ cell differentiation in Drosophila ovary. However, whether it functions in regulating the metabolic state of the host remains largely unknown. Methods We utilized GC-MS, qPCR, and some classical kits to examine various metabolic profiles and gut microbial composition in bam loss-of-function mutants and age-paired controls. We performed genetic manipulations to explore the tissue/organ-specific role of bam in regulating energy metabolism in Drosophila. The DSS-induced mouse colitis was generated to identify the role of Gm114, the mammalian homolog of bam, in modulating intestinal homeostasis. Results We show that loss of bam leads to an increased storage of energy in Drosophila. Silence of bam in intestines results in commensal microbial dysbiosis and metabolic dysfunction of the host. Moreover, recovery of bam expression in guts almost rescues the obese phenotype in bam loss-of-function mutants. Further examinations of mammalian Gm114 imply a similar biological function in regulating the intestinal homeostasis and energy storage with its Drosophila homolog bam. Conclusion Our studies uncover a novel biological function of bam/Gm114 in regulating the host lipid homeostasis.

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Section: Resultsmentioning

confidence: 99%

A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila

Wang

Zhu

Zhang

et al. 2022

PeerJ

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“…We show that flies consume very little food containing 2% H 2 O 2 . This effect is not limited to H 2 O 2 , as many supplements have been found to reduce feeding in flies [97], and this may extend to other oxidative stressors including paraquat and menadione. While several studies have detected reduced feeding in response to paraquat-containing food [102,103], others detected no difference [67].…”

Section: Starvation As a Confounding Factor In Stress Assaysmentioning

confidence: 89%

“…Feeding is essential to the survival of adult Drosophila, and feeding behavior is influenced by a variety of cues, including food acidity or the presence of bitter compounds, hypoxia, and the nutrient content of food [97][98][99][100]. While some stressors affect the preference for food of particular composition [98,101], others may alter feeding behavior by affecting satiety [97]. We show that flies consume very little food containing 2% H 2 O 2 .…”

Section: Starvation As a Confounding Factor In Stress Assaysmentioning

confidence: 99%

The metabolome as a link in the genotype-phenotype map for peroxide resistance in the fruit fly, Drosophila melanogaster

et al. 2020

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Background Genetic association studies that seek to explain the inheritance of complex traits typically fail to explain a majority of the heritability of the trait under study. Thus, we are left with a gap in the map from genotype to phenotype. Several approaches have been used to fill this gap, including those that attempt to map endophenotype such as the transcriptome, proteome or metabolome, that underlie complex traits. Here we used metabolomics to explore the nature of genetic variation for hydrogen peroxide (H2O2) resistance in the sequenced inbred Drosophila Genetic Reference Panel (DGRP). Results We first studied genetic variation for H2O2 resistance in 179 DGRP lines and along with identifying the insulin signaling modulator u-shaped and several regulators of feeding behavior, we estimate that a substantial amount of phenotypic variation can be explained by a polygenic model of genetic variation. We then profiled a portion of the aqueous metabolome in subsets of eight ‘high resistance’ lines and eight ‘low resistance’ lines. We used these lines to represent collections of genotypes that were either resistant or sensitive to the stressor, effectively modeling a discrete trait. Across the range of genotypes in both populations, flies exhibited surprising consistency in their metabolomic signature of resistance. Importantly, the resistance phenotype of these flies was more easily distinguished by their metabolome profiles than by their genotypes. Furthermore, we found a metabolic response to H2O2 in sensitive, but not in resistant genotypes. Metabolomic data further implicated at least two pathways, glycogen and folate metabolism, as determinants of sensitivity to H2O2. We also discovered a confounding effect of feeding behavior on assays involving supplemented food. Conclusions This work suggests that the metabolome can be a point of convergence for genetic variation influencing complex traits, and can efficiently elucidate mechanisms underlying trait variation.

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Section: Starvation As a Confounding Factor In Stress Assaysmentioning

confidence: 89%

The metabolome as a link in the genotype-phenotype map for peroxide resistance in the fruit fly, Drosophila melanogaster

Harrison

Wang

Gajda

et al. 2019

Preprint

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Background Genetic association studies that seek to explain the inheritance of complex traits typically fail to explain more than a small fraction of the heritability of the trait under study. Thus we are left with a gap in the map from genotype to phenotype. Several approaches have been used to fill this gap, including those that attempt to map endophenotype such as the transcriptome, proteome or metabolome, that underlie complex traits. Here we used metabolomics to explore the nature of genetic variation for hydrogen peroxide (H2O2) resistance in the sequenced inbred Drosophila Genetic Reference Panel (DGRP). Results We first studied genetic variation for H2O2 resistance in 180 DGRP lines and identify the insulin signaling modulator u-shaped and several regulators of feeding behavior. We then profiled a portion of the aqueous metabolome in subsets of eight ‘high resistance’ lines and eight ‘low resistance’ lines. We used these lines to represent collections of genotypes that were either resistant or sensitive to the stressor, effectively modeling a discrete trait. Across the range of genotypes in both populations, flies exhibited surprising consistency in their metabolomic signature of resistance. Metabolomic profiles were also able to distinguish stress-resistant from stress-sensitive flies with greater accuracy than the genotype of these same lines. Furthermore, we found a metabolic response to H2O2 that was shared among sensitive, but not resistant genotypes. Metabolomic data further implicated at least two pathways, glycogen and folate metabolism, as determinants of sensitivity to H2O2. We also discovered a confounding effect of feeding behavior on assays involving supplemented food. Conclusions This work suggests that the metabolome can be a point of convergence for genetic variation influencing complex traits, and efficiently elucidate the mechanisms underlying this trait variation.

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Central and Peripheral Regulation of Appetite and Food Intake in Drosophila

Cited by 9 publications

References 1 publication

A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila

A conserved role of bam in maintaining metabolic homeostasis via regulating intestinal microbiota in Drosophila

The metabolome as a link in the genotype-phenotype map for peroxide resistance in the fruit fly, Drosophila melanogaster

The metabolome as a link in the genotype-phenotype map for peroxide resistance in the fruit fly, Drosophila melanogaster

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