Central and peripheral administration of endothelin-1 induces an increase in blood pressure in conscious trout

Mével, Jean-Claude Le; Delarue, Catherine; Mabin, D; Vaudry, Hubert

doi:10.1152/ajpregu.1999.276.4.r1010

Cited by 7 publications

(11 citation statements)

References 25 publications

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“…Furthermore, hypercortisolemia has related with elevated ET-1 levels (Lederbogen et al 1999). The current data disagree with (le Mevel et al 1999) demonstrated that intraarterial injection of ET-1 did not markedly modify cortisol levels. Also, (Vierhapper et al 1995) concluded that cortisol concentration was unchanged by infusion of ET-1.…”

Section: Discussioncontrasting

confidence: 93%

Effect of Bosentan and Losartan on Oxidative Stress and Cortisol level in Endothelin-1 and Angiotensin II Treated Rats

2016

ZJPAS

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Section: Discussioncontrasting

confidence: 93%

Effect of Bosentan and Losartan on Oxidative Stress and Cortisol level in Endothelin-1 and Angiotensin II Treated Rats

2016

ZJPAS

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“…The cannula was immediately flushed with 60 l of vehicle (0.9% NaCl). The ET-1 solution was freshly prepared from a stock solution (10 Ϫ4 M), and the dose was chosen from the dose-response curves reported in the literature (28,56). Triton X-100 was added to prevent the adsorption of ET-1 to the tube wall.…”

Section: Methodsmentioning

confidence: 99%

FHL5, a novel actin-binding protein, is highly expressed in eel gill pillar cells and responds to wall tension

Mistry

Kato²,

Tran³

et al. 2004

American Journal of Physiology-Regulatory, Integrative and Comp

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Supporting evidence for the contractile nature of fish branchial pillar cells was provided by demonstrating the presence of actin fibers and a novel four-and-a-half LIM (FHL) protein in which expression is specific for contractile tissues and sensitive to the tension applied to the pillar cell. When eel gill sections were stained with rhodamine-phalloidin, a selective fluorescent probe for fibrous actin, a strong bundle-like staining was observed around collagen columns in pillar cells, suggesting the presence of abundant actin fibers. A cDNA clone encoding a novel member of the actin-binding FHL family, FHL5, was isolated from a subtracted cDNA library of eel gill. Northern analysis revealed that FHL5 mRNA is highly expressed only in gills, heart, and skeletal muscle. In gills, FHL5 was found to be confined to pillar cells by immunohistochemistry. Confocal fluorescence microscopy showed that FHL5 is present in both cytosol and nucleus; within the cytosol, a large portion of FHL5 is colocalized with the phalloidin-positive actin bundles. Furthermore, transfection of myogenic C2C12 cells with FHL5 cDNA demonstrated, in addition to its interaction with actin stress fibers, a nuclear shuttling activity of FHL5. The mRNA and protein levels were found to be elevated on 1) transfer of eels from seawater to freshwater, 2) volume expansion by infusion of isotonic dextran-saline, and 3) constriction of gill vasculature by bolus injection of endothelin-1. These results suggest contractile nature of pillar cells and a role of FHL5 in maintaining the integrity and regulating the dynamics of pillar cells. C2C12 myoblast; endothelin; immunohistochemistry; lamella; zinc finger; four-and-a-half LIM FISH GILLS CONSIST OF a large number of filaments arranged along the gill arches. The surfaces of the filaments are greatly enlarged by a series of plate-like lamellae. Each lamella is composed of two sheets of epithelia separated by a thin space through which the blood circulates to allow the exchange of respiratory gases. The separation between the epithelial sheets is maintained by pillar cells and collagen bundles. Pillar cells are spool-shaped cells connecting two epithelial sheets of the respiratory lamella in fish gills (50,51,64). They are characterized by collagen bundles contained in the infoldings of the plasma membrane and are oriented perpendicularly to the epithelial sheets, which consist of a thin layer of pavement cells and a basal lamina that is continuous with the collagen bundles traversing the pillar cells (49, 64); for the anatomy of gill lamella, see the schematic illustration in Fig. 9D. The membrane-enclosed collagen bundles help prevent ballooning of the lamella. The inner surfaces of the two epithelial sheets are covered with flanges extended from the pillar cells, forming capillary lumen called lacunae. Another feature of the pillar cells is the presence of numerous myofilament-like structures that course through the cytoplasm in an orientation parallel to the collagen bundles. These myofilaments appear to ...

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“…The potent hemodynamic effects of endothelin-1, such as increase in blood pressure (37,35), could also be important in vivo. In previous studies with the teleost rainbow trout, endothelin-1 has been found to cause several short-term oscillations in blood pressure followed by a sustained hypertensive period (19,15,6). In mammals, it has been found that locally induced vasoactive agents, such as endothelin-1, mediate, in a paracrine fashion, part of the stretch-induced ANP secretion (29).…”

Section: Discussionmentioning

confidence: 98%

Pronatriuretic peptide is a sensitive marker of the endocrine function of teleost heart

Vierimaa¹,

Hirvinen²,

Tervonen³

et al. 2002

American Journal of Physiology-Endocrinology and Metabolism

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We recently characterized a novel heart-specific hormone from salmon (salmon cardiac peptide, sCP). We have now prepared a recombinant plasmid expressing the NH2-terminal fragment of pro-sCP (NT-pro-sCP) and used it to set up a specific RIA for the peptide. Because of the sensitivity of the assay and the high circulating levels, NTpro-sCP can be measured from as little as 2 l of serum. This enables repeated sampling from the same animal in different experimental setups. Mechanical load increased the release of NT-pro-sCP from isolated perfused salmon ventricle, in parallel with sCP. Bolus injection of human endothelin-1 (ET-1; 1 g) in the dorsal aorta of salmon resulted in an extensive increase of serum NT-pro-sCP (from 0.99 Ϯ 0.11 to 4.6 Ϯ 1.5 nmol/l). The response was abolished by pretreatment with a specific type A ET (ETA) receptor antagonist (BQ-123) but not with a type B ET receptor antagonist (BQ-788).The NT-pro-sCP levels had a good correlation with those of sCP (r 2 ϭ 0.75). Our results demonstrate the practical usefulness of circulating NT-pro-sCP as a marker of the endocrine function of salmon heart. They also suggest that ET-1 has an important role in regulating sCP release from teleost heart by an ETA receptor-mediated mechanism. natriuretic peptide; endothelin; recombinant protein; radioimmunoassay; study in vivo NATRIURETIC PEPTIDES are cardiac hormones with an important role in regulating cardiovascular and fluid homeostasis (2). The release of A-and B-type natriuretic peptides (ANP and BNP) from the heart is stimulated by increased load (11,14,18) and by various paracrine factors, such as endothelin-1 (3, 16, 30). They reduce the cardiac load by causing natriuresis, diuresis, and relaxation of the vascular smooth muscle and by inhibiting the renin-angiotensin-aldosterone system (21). Although blood volume expansion is an important stimulus for ANP secretion in mammals, in nonmammalian eel, ANP secretion has been reported to be more sensitive to osmotic than volemic stimuli. In this teleost, ANP causes excretion of Na ϩ , thereby promoting adaptation to seawater (9).In mammals, ANP is stored in the secretory granules of atrial myocytes as a large-molecular-weight prohormone (proANP). It is processed to low-molecularweight ANP during exocytosis (33), presumably by the membrane-bound serine protease corin (36). The products are the 28-amino-acid biologically active ANP and the inert 98-amino-acid NH 2 -terminal fragment NTproANP (8,31). The elimination of ANP from the circulation is very rapid, resulting in low and labile plasma concentrations. The half-life of rat NT-proANP in rat circulation has been reported to be eight times longer than that of ANP (32). Therefore, and because its plasma concentrations are much higher, the measurement of NT-proANP is preferred over that of ANP in the assessment of cardiac function, e.g., in patients with congestive heart failure (22). The same appears to apply for .We have recently cloned and characterized from salmon a novel peptide hormone, salmon cardiac pep...

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Central and peripheral administration of endothelin-1 induces an increase in blood pressure in conscious trout

Cited by 7 publications

References 25 publications

Effect of Bosentan and Losartan on Oxidative Stress and Cortisol level in Endothelin-1 and Angiotensin II Treated Rats

Effect of Bosentan and Losartan on Oxidative Stress and Cortisol level in Endothelin-1 and Angiotensin II Treated Rats

FHL5, a novel actin-binding protein, is highly expressed in eel gill pillar cells and responds to wall tension

Pronatriuretic peptide is a sensitive marker of the endocrine function of teleost heart

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