Central actions of glucocorticoids in the control of body fluid homeostasis: Review

Ruginsk, Sílvia Graciela; Silva, A. Lopes da; Ventura, Raissa Wihby; Elias, Lucila Leico Kagohara; Antunes‐Rodrigues, José

doi:10.1590/s0100-879x2009000100010

Cited by 11 publications

(5 citation statements)

References 52 publications

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“…This approach prevents the large pool of Avp in MCNs masking the changes in gene expression in the smaller population of PCNs. However, our finding of DEX inhibition of Avp expression in both PVN and SON support the concept of DEX modulation of Avp expression may not only be evident in PCNs, but also in MCNs consistent with proposed functions of glucocorticoids in the control of water homeostasis [ 45 ]. It is known that modulation of excitatory and inhibitory synaptic transmission by glucocorticoids in the mouse PVN and SON is lost with conditional deletion of the glucocorticoid receptor [ 46 ].…”

Section: Discussionsupporting

confidence: 88%

Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus

et al. 2015

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BackgroundArginine vasopressin (AVP), a neuropeptide hormone that functions in the regulation of water homeostasis by controlling water re-absorption at kidneys, is synthesised in supraoptic nucleus and paraventricular nucleus of the hypothalamus. An increase in plasma osmolality stimulates secretion of AVP to blood circulation and induces AVP synthesis in these nuclei. Although studies on mechanism of AVP transcriptional regulation in hypothalamus proposed that cAMP and glucocorticoids positively and negatively regulate Avp expression, respectively, the molecular mechanisms have remained elusive. Recently, we identified CREB3L1 (cAMP-responsive element binding protein 3 like 1) as a putative transcription factor of Avp transcription in the rat hypothalamus. However the mechanism of how CREB3L1 is regulated in response of hyperosmotic stress in the neurons of hypothalamus has never been reported. This study aims to investigate effect of previously reported regulators (cAMP and glucocorticoid) of Avp transcription on transcription factor CREB3L1 in order to establish a molecular explanation for cAMP and glucocorticoids effect on AVP expression.ResultsThe effect of cAMP and glucocorticoid treatment on Creb3l1 was investigated in both AtT20 cells and hypothalamic organotypic cultures. The expression of Creb3l1 was increased in both mRNA and protein level by treatment with forskolin, which raises intracellular cAMP levels. Activation of cAMP by forskolin also increased Avp promoter activity in AtT20 cells and this effect was blunted by shRNA mediated silencing of Creb3l1. The forskolin induced increase in Creb3l1 expression was diminished by combined treatment with dexamethasone, and, in vivo, intraperitoneal dexamethasone injection blunted the increase in Creb3l1 and Avp expression induced by hyperosmotic stress.ConclusionHere we shows that cAMP and glucocorticoid positively and negatively regulate Creb3l1 expression in the rat hypothalamus, respectively, and regulation of cAMP on AVP expression is mediated through CREB3L1. This data provides the connection between CREB3L1, a newly identified transcription factor of AVP expression, with the previously proposed mechanism of Avp transcription which extends our understanding in transcription regulation of Avp in the hypothalamus.

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Section: Discussionsupporting

confidence: 88%

Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus

et al. 2015

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“…Glucocorticoids have been implicated in the regulation of oxytocin (OT) synthesis and secretion in response to altered fluid volume and tonicity (17). Glucocorticoid receptor (GR) signaling is important for normal heart function and development (18).…”

Section: Introductionmentioning

confidence: 99%

The Role of Glucocorticoid Receptor and Oxytocin Receptor in the Septic Heart in a Clinically Relevant, Resuscitated Porcine Model With Underlying Atherosclerosis

et al. 2020

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The pathophysiology of sepsis-induced myocardial dysfunction is not resolved to date and comprises inflammation, barrier dysfunction and oxidative stress. Disease-associated reduction of tissue cystathionine-γ-lyase (CSE) expression, an endogenous H 2 S-producing enzyme, is associated with oxidative stress, barrier dysfunction and organ injury. CSE-mediated cardio-protection has been suggested to be related the upregulation of oxytocin receptor (OTR). CSE can also mediate glucocorticoid receptor (GR) signaling, which is important for normal heart function. A sepsis-related loss of cardiac CSE expression associated with impaired organ function has been reported previously. The aim of this current post hoc study was to investigate the role of cardiac GR and OTR after polymicrobial sepsis in a clinically relevant, resuscitated, atherosclerotic porcine model. Anesthetized and instrumented FBM (Familial Hypercholesterolemia Bretoncelles Meishan) pigs with high fat diet-induced atherosclerosis underwent poly-microbial septic shock (n = 8) or sham procedure (n = 5), and subsequently received intensive care therapy with fluid and noradrenaline administration for 24 h. Cardiac protein expression and mRNA levels were analyzed. Systemic troponin, a marker of cardiac injury, was significantly increased in septic animals in contrast to sham, whereas OTR and GR expression in septic hearts were reduced, along with a down-regulation of anti-inflammatory GR target genes and the antioxidant transcription factor NRF2. These results suggest a potential interplay between GR, CSE, and OTR in sepsis-mediated oxidative stress, inflammation and cardiac dysfunction.

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“…The fast effects of the treatment on water search behavior in the toads can also be explained by non-genomic CORT effects. ANG II and GC modulate the synthesis and secretion of neurohypophyseal hormones, particularly oxytocin, in rats exposed to different experimental conditions of altered fluid volume and tonicity (Ruginsk et al 2009). The underlying mechanisms by which CORT administration results in rapid behavioral changes observed in our toads remain to be tested.…”

Section: Discussionmentioning

confidence: 99%

Challenges of dehydration result in a behavioral shift in invasive toads

Madelaire

Barsotti

Wagener

et al. 2020

Behav Ecol Sociobiol

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The adaptive nature of invasive species facilitates their survival in conditions that differ markedly from their native range. Behavioral changes in invasive populations are poorly explored but offer a wide potential when combined with physiological traits. For amphibians invading xeric habitats, finding water is important to function optimally and avoid dehydration. The waterfinding hypothesis postulates that survival can be enhanced through the increased behavioral ability to find water. We tested the water-finding hypothesis in guttural toads (Sclerophrys gutturalis) from their native range and an invasive population. Additionally, we tested if artificially elevated corticosterone levels, which increase during dehydration, affect behavioral traits linked to water balance. In a labyrinth experiment, we observed the toads' ability to find water in different hydration states (100%, 90%, and 80%). We found that individuals from the invasive population took longer to engage in water-searching behavior and spent more time close to the water source after finding it. Toads from the invasive population were also more active, and at 90% hydration, their attempts to find water increased. Moreover, artificially increasing corticosterone in fully hydrated invasive toads increased water-finding success. Our experiments demonstrated that invasive toads show water-conservation behaviors that can optimize water balance and might facilitate survival in an invaded xeric environment. Additionally, we suggest a link between elevated corticosterone levels and water-finding success. Our results lend support to the importance of behavior in successful invasions and the modulation of water-finding behavior by corticosterone. Significant statementInvasion success is associated with behavioral and physiological adaptations of invasive species. For anuran species invading xeric habitats, the ability to find water is an important behavioral trait. Using a labyrinth experiment and toads (Sclerophrys gutturalis) dehydrated at different levels, we observed that individuals invading a hydric challenging habitat showed advantageous water-conservation behaviors (e.g., taking longer to engage in water-searching behavior and spent more time close to the water source after finding it) compared to toads from the native range. Transdermal treatment with corticosterone, a hormone that increases its plasma concentrations under dehydration, increased success of finding water in 100% hydrated animals. Our results indicate that, compared to the native population, invasive toads display water-conservation behaviors that might improve survival rates in a novel xeric environment and that experimental corticosterone elevation modulates this water-finding behavior.

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Central actions of glucocorticoids in the control of body fluid homeostasis: Review

Cited by 11 publications

References 52 publications

Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus

Transcription factor CREB3L1 mediates cAMP and glucocorticoid regulation of arginine vasopressin gene transcription in the rat hypothalamus

The Role of Glucocorticoid Receptor and Oxytocin Receptor in the Septic Heart in a Clinically Relevant, Resuscitated Porcine Model With Underlying Atherosclerosis

Challenges of dehydration result in a behavioral shift in invasive toads

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