Centractin is an actin homologue associated with the centrosome

Clark, Spencer K.; Meyer, David I.

doi:10.1038/359246a0

Cited by 163 publications

(139 citation statements)

References 32 publications

Supporting

Mentioning

133

Contrasting

Unclassified

Order By: Relevance

“…Similarly, actin and centractin are about 50% identical [2,3]. By contrast, the homologies between members of the tubulin and actin families are restricted to a small number of peptides, most of which (such as a glycine-rich peptide) are common to many nucleotidebinding proteins.…”

Section: Introductionmentioning

confidence: 99%

Functional role of a consensus peptide which is common to α‐, β‐, and γ‐tubulin, to actin and centractin, to phytochrome A, and to the TCP1α chaperonin protein

Burns¹,

Surridge²

1994

FEBS Letters

View full text Add to dashboard Cite

The TRiC (TCPl Ring Complex) chaperonin complex participates in the functional folding of actin, centractin, a-,/J-, y-tubulin, and phytochrome. Each of the cytoskeletal proteins contain a peptide, RK(A,C,T)F/KRAF, located towards the C-terminus, which is homologous to a TCPld peptide, while the equivalent phytochrome peptide (RLKAF in certain isoforms) is very similar to the KLRAF peptide of TCPla. We propose that this TCPla peptide binds to the nascent polypeptides as they emerge from the ribosome, that this binding restricts the folding pathway, and that the TCPla peptide is subsequently displaced by the synthesis of the consensus peptide. This hypothesis is strongly supported by the crystallographic structure of actin.

show abstract

Section: Introductionmentioning

confidence: 99%

Functional role of a consensus peptide which is common to α‐, β‐, and γ‐tubulin, to actin and centractin, to phytochrome A, and to the TCP1α chaperonin protein

Burns¹,

Surridge²

1994

FEBS Letters

View full text Add to dashboard Cite

show abstract

“…Recently centractin, an actin-like protein found in the centrosome, was thought to be involved in the association between centrioles and the microfilament network (Clark and Meyer, 1992). Therefore, microfilaments may also be involved in the process which leads to the perpendicular orientation of centrioles.…”

Section: Introductionmentioning

confidence: 99%

Centrosome Behavior under the Action of a Mitochondrial Uncoupler and the Effect of Disruption of Cytoskeleton Elements on the Uncoupler-Induced Alterations

Alieva

Vorobjev

1994

Journal of Structural Biology

View full text Add to dashboard Cite

Carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone (FCCP) induced in pig kidney embryo cells a loss of rhodamine 123 staining of mitochondria in 2-3 min. Within 5 min after FCCP inoculation of cells prestained with rhodamine 123, the diffuse staining of the cytoplasm was absent. FCCP did not induce changes in the cytoplasmic microtubule complex, but induced nonrandom (preferentially perpendicular to the substrate surface) orientation of maternal centrioles. Nonrandom orientation of maternal centrioles occurred 10 min after treatment and remained for 2 hr. At 30 min after introduction of the drug, FCCP treatment increased the mean number of pericentriolar satellites on maternal centrioles and the frequency of primary cilia. The percentage of centrioles perpendicular to the substrate induced by FCCP treatment was slightly increased by disruption of microtubules and slightly diminished by disruption of microfilaments. In both cases centrioles were oriented signifycantly differently from random (P < 0.01). These results suggest that microtubules are neither involved in the signaling pathway from plasma membrane to the centriole, nor do they anchor the centrioles perpendicular to the substrate, as proposed

show abstract

“…Arpl: vertebrate actin-RPV* ( Lees-Miller et al, 1992b), vertebrate centractin* (Clark and Meyer, 1992), vertebrate /~-centractin , Drosophila Arp87C (Fyrberg et al, 1994), Neurospora Arpl (Plamann et al, 1994) Pneumocystis Arpl (Christopher et al, 1994), Saccharomyces Act3p** , Saccharomyces Act5p** (Muhua et al, 1994), and Caenorhabditis ActB (Waterston, R., D. Helfman, personal communication).…”

mentioning

confidence: 99%

“…In the summary below we group the various proteins according to this classification scheme. Identical superscripts designate identical proteins.Arpl: vertebrate actin-RPV* ( Lees-Miller et al, 1992b), vertebrate centractin* (Clark and Meyer, 1992), vertebrate /~-centractin (Clark et al …”

mentioning

confidence: 99%

See 1 more Smart Citation

Actin-related protein nomenclature and classification.

Schroer

Fyrberg

Cooper

et al. 1994

The Journal of Cell Biology

View full text Add to dashboard Cite

ECENTLY several laboratories have discovered genes or cDNAs encoding proteins that are 35-55 % identical to conventional actin isoforms. The initial discoveries, largely serendipitous, were unexpected, as for some time it had been thought that actin was a unique protein.These actin-related proteins apparently play profound roles in cells. Those discovered to date have been shown to be essential or important in yeasts (Lees-Miller et al., 1992a;Schwob and Martin, 1992;Muhua et al., 1994;Harata et al., 1994), and one is known to copolymerize with actin in vivo (Schafer et al., 1994) to form a short, discrete microfilament. The emerging consensus is that there are only a few such proteins, and that they are highly conserved in all eukaryotes, from protozoa to yeasts to mammals.Functional studies of the actin-related proteins are proceeding rapidly. It was suspected from the time of their initial discovery that actin-relatod proteins would have distinct cellular roles, for while their similarities to actins are striking, the novel proteins are much less abundant, and in many instances have peptide insertions never found within actin isoforms. Indeed, the best characterized actin-related protein, referred to by various authors as actin-RPV, centractin, Saccharomyces cerevisiae Act5 protein, and S. cerevisiae Act3 protein (Lees-Miller et al., 1992b; Meyer, 1992, 1994;Muhua et al., 1994), has been found in vertebrates to form a short filament that facilitates movemonts of vesicles on microtubules by cytoplasmic dynein (Lees-Miller et al., 1992b;Paschal et al., 1993;Schafer et al., 1994), and in yeast and filamentous fungi to implement mitotic spindle orientation and nuclear migration (Muhua et al., 1994; Plamann et al., 1994). In Acanthamoeba, two different actin-related proteins are found in a complex, four members of which bind to profilinSepharose (Machesky ot al., 1994). The importance of these actin relatives now seems well established, but much remains to be learned.In the course of analyzing primary sequences of actinrelated proteins from several sources we have noted that Address all correspondence to E. Fyrberg, Department of Biology, Johns Hopkins University, 144 Mudd Hall, 3400 N. Charles Street, Baltimore, MD 21218-2685. Tel.: (410) 516-5213. three families are widely distributed. As might be expected, very similar, and in some cases identical, proteins have been given a variety of names. Both the random naming and the fact that actin-related proteins are not designated so as to differentiate them from conventional actins have created confusion within the literature. Therefore, we feel that it is essential to devise a more unifying nomenclature for actinrelated proteins, and also to point out the apparent subsets of equivalent proteins defined by us independently using phylogenetic tree analysis programs, such as CLUSTAL V.We propose designating actin-related proteins with the Arp (actin-related protein) prefix. Of the currently known families, the one that is most similar to conventional actin (•4...

show abstract

Centractin is an actin homologue associated with the centrosome

Cited by 163 publications

References 32 publications

Functional role of a consensus peptide which is common to α‐, β‐, and γ‐tubulin, to actin and centractin, to phytochrome A, and to the TCP1α chaperonin protein

Functional role of a consensus peptide which is common to α‐, β‐, and γ‐tubulin, to actin and centractin, to phytochrome A, and to the TCP1α chaperonin protein

Centrosome Behavior under the Action of a Mitochondrial Uncoupler and the Effect of Disruption of Cytoskeleton Elements on the Uncoupler-Induced Alterations

Actin-related protein nomenclature and classification.

Contact Info

Product

Resources

About