Centennial celebration of the bacteriophage research

Debarbieux, Laurent; Forterre, Patrick; Krupovìč, Mart; Kutateladze, Mzia; Prangishvili, David

doi:10.1016/j.resmic.2018.10.001

Cited by 2 publications

(2 citation statements)

References 34 publications

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“…Phages were discovered over a century ago, and phage therapy has a long history (Debarbieux et al, 2018; Gelman et al, 2018). However, the advent of chemical antibiotics led to virtual abandonment of phage therapy in most of the world, whereas in the countries of Eastern Europe phage therapy was continuously pursued (Chanishvili, 2016; Myelnikov, 2018).…”

Section: Introductionmentioning

confidence: 99%

Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections

et al. 2019

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Phage therapy requires libraries of well-characterized phages. Here we describe the generation of phage libraries for three target species: Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae. The basic phage characteristics on the isolation host, sequence analysis, growth properties, and host range and virulence on a number of contemporary clinical isolates are presented. This information is required before phages can be added to a phage library for potential human use or sharing between laboratories for use in compassionate use protocols in humans under eIND (emergency investigational new drug). Clinical scenarios in which these phages can potentially be used are discussed. The phages presented here are currently being characterized in animal models and are available for eINDs.

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Section: Introductionmentioning

confidence: 99%

Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections

et al. 2019

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“…Similar to these two phages, IME-JL8 was also highly specific to the bacteria, only sensitive to their host bacteria. Moreover, antibiotic resistance genes or putative virulence factors were not reported in this phage by genomics analysis (Debarbieux et al, 2018;Santos et al, 2018;Straus and Bo, 2018). All the mentioned factors strongly suggest that IME-JL8 could be a potential therapeutic phage against multiple C. freundii infections.…”

Section: Discussionmentioning

confidence: 92%

Genomic, Morphological and Functional Characterization of Virulent Bacteriophage IME-JL8 Targeting Citrobacter freundii

et al. 2020

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Citrobacter freundii refers to a fish pathogen extensively reported to be able to cause injury and high mortality. Phage therapy is considered a process to alternatively control bacterial infections and contaminations. In the present study, the isolation of a virulent bacteriophage IME-JL8 isolated from sewage was presented, and such bacteriophage was characterized to be able to infect Citrobacter freundii specifically. Phage IME-JL8 has been classified as the member of the Siphoviridae family, which exhibits the latent period of 30–40 min. The pH and thermal stability of phage IME-JL8 demonstrated that this bacteriophage achieved a pH range of 4–10 as well as a temperature range of 4, 25, and 37°C. As revealed from the results of whole genomic sequence analysis, IME-JL8 covers a double-stranded genome of 49,838 bp (exhibiting 47.96% G+C content), with 80 putative coding sequences contained. No bacterial virulence- or lysogenesis-related ORF was identified in the IME-JL8 genome, so it could be applicable to phage therapy. As indicated by the in vitro experiments, phage IME-JL8 is capable of effectively removing bacteria (the colony count decreased by 6.8 log units at 20 min), and biofilm can be formed in 24 h. According to the in vivo experiments, administrating IME-JL8 (1 × 107 PFU) was demonstrated to effectively protect the fish exhibiting a double median lethal dose (2 × 109 CFU/carp). Moreover, the phage treatment led to the decline of pro-inflammatory cytokines in carp with lethal infections. IME-JL8 was reported to induce efficient lysis of Citrobacter freundii both in vitro and in vivo, thereby demonstrating its potential as an alternative treatment strategy for infections attributed to Citrobacter freundii.

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Centennial celebration of the bacteriophage research

Cited by 2 publications

References 34 publications

Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections

Constructing and Characterizing Bacteriophage Libraries for Phage Therapy of Human Infections

Genomic, Morphological and Functional Characterization of Virulent Bacteriophage IME-JL8 Targeting Citrobacter freundii

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