Centchroman regulates breast cancer angiogenesis via inhibition of HIF-1α/VEGFR2 signalling axis

Dewangan, Jayant; Kaushik, Shweta; Rath, Srikanta Kumar; Balapure, Anil K.

doi:10.1016/j.lfs.2017.11.045

Cited by 38 publications

(23 citation statements)

References 37 publications

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“…Angiogenesis is an important factor favouring tumour development. 37 We therefore analysed the content levels of VEGF, a proangiogenic factor, and IL12p40, an anti-angiogenic factor, 38 in peritumoural adipose tissue. IL12p40 was lower in the CC group than in the WSC group, but there was no difference between the groups in VEGF content.…”

Section: Discussionmentioning

confidence: 99%

Peritumoural adipose tissue pro‐inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

Neto

Murari

Oyama

et al. 2018

J cachexia sarcopenia muscle

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BackgroundCancer cachexia (CC) is a multifactorial syndrome, often irreversible, that affects patients with cancer influenced, in part, by the inflammatory condition. Peritumoural adipose tissue produces adipokines and angiogenic, apoptotic, and growth factors; given the possible crosstalk between the peritumoural adipose tissue and tumour, these may play an important role in cancer biology and carcinogenesis.MethodsThe aim of this study was to evaluate the factors produced by peritumoural adipose tissue in a cohort of 16 colorectal cancer patients with either weight‐stable cancer (WSC; n = 7) or CC (n = 9). The study was approved by the Ethics Research Committee (972.914). Samples of peritumoural adipose tissue were analysed for concentrations of TNF‐α, IL‐1β, STAT‐1, STAT‐3, RANTES, IL‐1Ra, IP‐10, IL‐15, MCP‐1, IFN‐α, GCSF, FADD, and TGF‐β. The cytokines and proteins were measured using Multiplex. Correlations between the proteins and cytokines were evaluated.ResultsTNF‐α, STAT‐1, and FADD, a factor involved in apoptosis, were significantly higher in CC group than in the WSC group. In the peritumoural adipose tissue of the CC group, RANTES showed a significant positive correlation with IL‐1Ra and IP‐10 and a negative correlation with IFN‐α; and GCSF showed significant negative correlations with IL‐1Ra, IP‐10, IL‐15, and MCP‐1 and a positive correlation with IFN‐α. In the peritumoural adipose tissue of the WSC group, no significant correlations were detected between RANTES, GCSF, IL‐3, FADD, and STAT‐1 and the cytokines/chemokines analysed.ConclusionsThese results indicated that inflammatory and tumorigenic pathways were altered in peritumoural adipose tissue in CC. Furthermore, inflammatory cytokines were correlated with growth factors in the peritumoural adipose tissue of cachectic patients, suggesting that inflammatory cytokines modulated the proliferative environment closely linked to the tumour.

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Section: Discussionmentioning

confidence: 99%

Peritumoural adipose tissue pro‐inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

Neto

Murari

Oyama

et al. 2018

J cachexia sarcopenia muscle

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“…Another transcription pathway that was also seen to be more expressed along with the high expression of HIF-1α was the PI3K/AKT pathway, which is important in signaling proliferation, invasion, angiogenesis, etc. [23] .…”

Section: Prognosis Value Of Hif-1α Expressionmentioning

confidence: 99%

The role of hypoxia-induced factor 1�� in breast cancer

Peiró¹,

Encina²,

Perez³

et al. 2019

JCMT

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Breast cancer usually grows very quickly, becoming insensitive to blood flow in nearby veins; because of that, inside solid tumors it's possible to find a hypoxic environment, in other words, an environment where oxygen is less available. Another feature of cancer is its angiogenesis rate, because of the high energy demand, new blood vessels must be produced to take nutrients inside the solid tumor mass. Even with normal blood flow bringing the cancer oxygen and nutrients, its cells favor hypoxia, in an event known as Warburg Effect. According to the Warburg Effect, cells, even with normal oxygen rates, prefer to use fermentation instead of the citric acid cycle to produce ATP. For the cancer to operate normally in hypoxia, a transcription factor family is activated, known as hypoxia-induced factors (HIF), composed of a HIF-1β and a HIF-1α subunits. As HIF-1α is expressed during hypoxia, it is a great target for treatments and a breast cancer biomarker. Because of the role of HIF-1α in cancer and the high incidence of breast cancer worlwide, this review was performed in order to bring the most recent results concerning the role HIF-1α can exert in breast cancer development and progression.

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“…MTT or BrdU cell proliferation assays have commonly been employed to assess the proliferation of endothelial cells (Staton et al, ). Endothelial cell (HUVEC) proliferation assay was used in the study conducted by Dewangan et al (). Bräutigam et al () have also conducted cell proliferation assays with human primary endothelial cells.…”

Section: Assays and Techniques Used To Monitor Angiogenesismentioning

confidence: 99%

“…Migratory cells can be observed with the help of a light microscope or by cell lysis (Staton et al, 2004). The effects of centchroman, a non-steroidal oral contraceptive, on the invasion of HUVECs have been monitored with the help of the Boyden chamber assay by Dewangan, Kaushik, Rath, and Balapure (2018). Bräutigam, Dieu, Heiss, Bischoff, and Fürst (2017) have reported effects of narciclasine, an alkaloid, in the reduction of migration of human primary endothelial cells with the Boyden chamber assay.…”

Section: Boyden Chamber Assaymentioning

confidence: 99%

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In vitro assays and techniques utilized in anticancer drug discovery

Ediriweera

Tennekoon

Samarakoon

2018

J of Applied Toxicology

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Development of a cancer is a multistep process and six major hallmarks of cancer that are known to control malignant transformation have been described. Anticancer drug development is a tedious process, requiring a number of in vitro, in vivo and clinical studies. In vitro assays provide an initial platform for cancer drug discovery approaches. A wide range of in vitro assays/techniques have been developed to evaluate each hallmark feature of cancer and selection of a particular in vitro assay or technique mainly depends on the specific research question (s) to be examined. In the present review, we have described some commonly utilized in vitro assays and techniques used to examine cell viability/proliferation, apoptosis, cellular senescence, invasion and migration, oxidative stress and antioxidant effects, gene and protein expression, angiogenesis and genomic alterations in cancer drug discovery. Additionally, uses of modern techniques such as high throughput screening, high content screening and reporter gene assays in cancer drug discovery have also been described.

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Centchroman regulates breast cancer angiogenesis via inhibition of HIF-1α/VEGFR2 signalling axis

Cited by 38 publications

References 37 publications

Peritumoural adipose tissue pro‐inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

Peritumoural adipose tissue pro‐inflammatory cytokines are associated with tumoural growth factors in cancer cachexia patients

The role of hypoxia-induced factor 1�� in breast cancer

In vitro assays and techniques utilized in anticancer drug discovery

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