CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.

Liao, Hong; Winkfein, Robert J.; Mack, G; Rattner, J. B.; Yen, Tim J.

doi:10.1083/jcb.130.3.507

Cited by 340 publications

(331 citation statements)

References 36 publications

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“…CENP-F (mitosin) associates with the centromere-kinetochore complex from the onset of mitosis to the metaphase (Rattner et al, 1993), gradually accumulates during the cell cycle and peaks in G 2 and M phase cells, and is then rapidly degraded upon the completion of mitosis (Liao et al, 1995). KIF4A belongs to the kinesinlike protein family, and is fundamental to many biological processes, including cell division, and the intracellular transport of membranous organelles in higher eukaryotic cells (Miki et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Exploration of replicative senescence-associated genes in human dermal fibroblasts by cDNA microarray technology

Yoon

Kim

et al. 2004

Experimental Gerontology

108

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Section: Discussionmentioning

confidence: 99%

Exploration of replicative senescence-associated genes in human dermal fibroblasts by cDNA microarray technology

Yoon

Kim

et al. 2004

Experimental Gerontology

108

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“…While it is now well established that regulated proteolysis of cell cycle proteins is crucial for orderly progression of the cycle (Minshull et al, 1989;Murray, 1989;Glotzer et al, 1991;Liao et al, 1995;Hsu et al, 1996), few studies have addressed the role of the well known and ubiquitous Ca 2+ -dependent proteases, calpains, in this process. It was previously demonstrated that thiol protease inhibitors like ALLN-CHO could inhibit the proliferative growth of vascular smooth muscle cells at G 1 and G 2 /M-phases (March et al, 1993).…”

Section: Calpain Inhibitors Block Progression To S-phasementioning

confidence: 99%

Inhibition of the growth of WI-38 fibroblasts by benzyloxycarbonyl-Leu-Leu-Tyr diazomethyl ketone: evidence that cleavage of p53 by a calpain-like protease is necessary for G1 to S-phase transition

et al. 1997

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The e ect of a calpain-selective cell permeant inhibitor, benzyloxycarbonyl Leu-Leu-Tyr diazomethylketone (ZLLY-CHN 2 ), on the serum-stimulated growth of WI-38 human ®broblasts has been investigated. Only cell permeant protease inhibitors with activity against calpains prevented progression into S-phase. Protein blotting experiments indicated that p53 immunoreactivity increased in late G 1 cells treated with ZLLY-CHN 2 . The content of p21 Waf1/Cip1 CDK inhibitor also increased, providing a mechanism for the observed failure to enter S-phase. Further studies indicated that p53 could be degraded by a ZLLY-CHN 2 -sensitive protease immediately prior to S-phase, but that proteolysis did not occur after this critical time point. Chelation of extracellular Ca 2+ by addition of EGTA inhibited the p53 degradation. Consistent with proteolysis of p53 in late G 1 phase, m-calpain immunoreactivity transiently accumulated in cell nuclei at this time. ZLLY-CHN 2 did not appear to increase p53 mRNA in WI-38 cells. Puri®ed mcalpain required only 1 to 3 mM Ca 2+ to proteolyze p53 in WI-38 cell lysates. These results indicate that ZLLY-CHN 2 inhibits progression of WI-38 cells into S-phase by inactivating a calpain-like protease that is responsible for proteolysis of constitutively expressed p53 in late G 1 .

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“…The attachment and proper positioning of chromosomes on the spindle likely involves cytoplasmic dynein (Echeverri et al 1996) andCENP-E (1997;Schaar et al 1997;Wood et al 1997), both of which are located in the outer plate and corona. The possible functions of other kinetochore components, including ZW10 (e.g., Starr et al 1997), CENP-F (also known as mitosin; Liao et al 1995) and MCAK (Wordeman and Mitchison 1995), remain to be determined. The composition of facultative proteins on the kinetochore changes during the course of mitosis.…”

Section: Introductionmentioning

confidence: 99%

A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution

et al. 1998

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Three decades of structural analysis have produced the view that the kinetochore in vertebrate cells is a disk-shaped structure composed of three distinct structural domains. The most prominent of these consists of a conspicuous electron opaque outer plate that is separated by a light-staining electrontranslucent middle plate from an inner plate associated with the surface of the pericentric heterochromatin. Spindle microtubules terminate in the outer plate and, in their absence, a conspicuous corona of fine filaments radiates from the cytoplasmic surface of this plate. Here we report for the first time the ultrastructure of kinetochores in untreated and Colcemid-treated vertebrate somatic (PtK 1 ) cells prepared for optimal structural preservation using high-pressure freezing and freeze substitution. In serial thin sections, and electron tomographic reconstructions, the kinetochore appears as a 50-75 nm thick mat of light-staining fibrous material that is directly connected with the more electron-opaque surface of the centromeric heterochromatin. This mat corresponds to the outer plate in conventional preparations, and is surrounded on its cytoplasmic surface by a conspicuous 100-150 nm wide zone that excludes ribosomes and other cytoplasmic components. High magnification views of this zone reveal that it contains a loose network of light-staining, thin (<9 nm diameter) fibers that are analogous to the corona fibers in conventional preparations. Unlike the chromosome arms, which appear uniformly electron opaque, the chromatin in the primary constriction appears mottled. Since the middle plate is not visible in these kinetochore preparations this feature is likely an artifact produced by extraction and coagulation during conventional fixation and/or dehydration procedures.

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CENP-F is a protein of the nuclear matrix that assembles onto kinetochores at late G2 and is rapidly degraded after mitosis.

Abstract: Abstract. Centromere protein-F (CENP-F) is a mammalian kinetochore protein that was recently identified by an autoimmune serum (Rattner,

Cited by 340 publications

References 36 publications

Exploration of replicative senescence-associated genes in human dermal fibroblasts by cDNA microarray technology

Exploration of replicative senescence-associated genes in human dermal fibroblasts by cDNA microarray technology

Inhibition of the growth of WI-38 fibroblasts by benzyloxycarbonyl-Leu-Leu-Tyr diazomethyl ketone: evidence that cleavage of p53 by a calpain-like protease is necessary for G1 to S-phase transition

A new look at kinetochore structure in vertebrate somatic cells using high-pressure freezing and freeze substitution

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