CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly

Shono, Nobuaki; Ohzeki, Jun Ichirou; Otake, Kazuo; Martins, Nuno M. C.; Nagase, Takahiro; Kimurâ, Hiroshi; Larionov, Vladimir; Earnshaw, William C.; Masumoto, Hiroshi

doi:10.1242/jcs.180786

Cited by 58 publications

(73 citation statements)

References 77 publications

(127 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In mammalian cells, the kinetochore protein CENP-C recruits epigenetic factors such as DNA methyltransferases or KNL2 to centromeric and pericentromeric satellite sequences to promote epigenetic maintenance of CENP-A chromatin (Gopalakrishnan et al, 2009;Shono et al, 2015). CENP-C has been shown to interact directly with KNL2 (Moree et al, 2011;Dambacher et al, 2012) and with cenH3 nucleosomes (Westermann et al, 2003;Carroll et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Targeting of Arabidopsis KNL2 to Centromeres Depends on the Conserved CENPC-k Motif in Its C Terminus

et al. 2017

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

“…The loss of CENP-C leads to chromosome misalignment and segregation defects during mitosis and to increased transcription of centromeric repeats. CENP-I can also recruit KNL2 independently of CENP-C in HeLa cells (Shono et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Targeting of Arabidopsis KNL2 to Centromeres Depends on the Conserved CENPC-k Motif in Its C Terminus

et al. 2017

View full text Add to dashboard Cite

“…Beyond their use as gene-delivery vectors, HACs have proven especially important in chromosome biology research, to quantify chromosome instability in cancer cells (Lee et al 2013;Duffy et al 2016;Kim et al 2016;Lee et al 2016), to study telomere maintenance (Wakai et al 2014) and to study the chromatin requirements for centromere function (Nakano et al 2008;Cardinale et al 2009;Bergmann et al 2011;Bergmann et al 2012a;Ohzeki et al 2012;Shono et al 2015;Ohzeki et al 2016;Martins et al 2016;Molina et al 2016b) ( Table 1). …”

Section: Human Artificial Chromosomes Development: a Historic Viewmentioning

confidence: 99%

“…The proper assembly of CCAN components, and in turn the outer kinetochore proteins, is dependent on the presence of CENP-A (Oegema et al 2001;Goshima et al 2003;Liu et al 2006;Hori et al 2013;Shono et al 2015). This suggested that CENP-A might act as an epigenetic mark responsible for the maintenance of centromere identity (Vafa and Sullivan 1997;Warburton et al 1997).…”

Section: Heterochromatin Versus Centrochromatin In Centromere Assemblmentioning

confidence: 99%

Using human artificial chromosomes to study centromere assembly and function

et al. 2017

Self Cite

View full text Add to dashboard Cite

Abstract:Centromeres are the site of assembly of the kinetochore, which directs chromosome segregation during cell division. Active centromeres are characterized by the presence of nucleosomes containing CENP-A and a specific chromatin environment that resembles that of active genes. Recent work using Human Artificial Chromosomes (HAC) sheds light on the fine balance of different histone post-translational modifications and transcription that exists at centromeres for kinetochore assembly and maintenance. Here, we review the use of HAC technology to understand centromere assembly and function. We put particular emphasis on studies using the alphoidtetO HAC, whose centromere can be specifically modified for epigenetic engineering studies. Author Comments:We suggest Professor Erich Nigg as an Editor (not found in the "Request Editor" list), who invited us to write this review.Response to Reviewers: Comments for the Author:Reviewer #1: Since there has been significant progress over the last few years with regards to HAC technology, particularly with alphoidtetO HACs, a review on this topic would be welcomed in the field, especially by these authors, who are world experts on Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporationthis topic. The review is mostly satisfying, but I would suggest two areas where the authors would be well served to make additions/changes to avoid disappointing readers hoping for more focus/organization/clarity. First, since the goal of this review is to "review the use of HAC technology to understand centromere assembly and function", it would be especially helpful if the authors summarized the evolution of HAC technology and the findings that came from targeting different tetR fusion proteins to the alphoidtetO HACs (probably most effectively in a table or schematic).We thank the reviewer for the thoughtful review of our MS. Following the reviewer´s advice, a table has been added explaining the findings that came from the alphoidtetO HAC studies in a timeline manner (Table 1).Second, more discussion on the pitfalls of current HACs would be desirable (currently only a couple sentences is mentioned in the future directions). From how it is written they sound so great that a reader would probably wonder why they are not used more widely in the research lab and/or clinic. There are problems with them, and it would be more clear and interesting, in my opinion, if more of the problems were laid out/challenges addressed.A description of limitations of the HAC technology have been added in the text, first limitations for HAC clinical use (p. 8-9) and additional limitations that should be overcome in the final remarks (p. 23). Some other changes that I'd suggest:1. It's confusing to refer to the same DNA as "alpha satellite", "α-satellite", and "alpha-satellite" throughout the review (most notably in the first two lines of the second paragraph). Why not just refer to it as "α-satellite" DNA?We changed the term to α-satellite DNA throughout the text to maintain consist...

show abstract

“…Interestingly, the priming of native centromeric chromatin is achieved by interactions of pre-existing CENP-A with other kinetochore components, including CENP-C or CENP-N (Carroll et al 2010;Hori et al 2013;Falk et al 2015;Shono et al 2015). This interaction recruits other kinetochore components and licensing factors (Figs.…”

Section: Priming the Centromerementioning

confidence: 99%

Kinetochore assembly and function through the cell cycle

Nagpal

Fukagawa

2016

Chromosoma

View full text Add to dashboard Cite

The kinetochore is an essential structure for the chromosome segregation machinery in eukaryotes; it serves as a bridge between the spindle microtubules and chromosomes. The kinetochore consists of multiple interconnecting components on the centromere; therefore, understanding its formation, molecular function, and regulation has remained an ongoing challenge. Recent studies have provided new insights into centromere identity, kinetochore assembly, and function. In this review, we discuss recent advances in our understanding of the function and regulation of key kinetochore components. We highlight the reciprocal localization dependencies of the different sub-complexes of the kinetochore and describe their regulation during the cell cycle.

show abstract

CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly

Cited by 58 publications

References 77 publications

Targeting of Arabidopsis KNL2 to Centromeres Depends on the Conserved CENPC-k Motif in Its C Terminus

Targeting of Arabidopsis KNL2 to Centromeres Depends on the Conserved CENPC-k Motif in Its C Terminus

Using human artificial chromosomes to study centromere assembly and function

Kinetochore assembly and function through the cell cycle

Contact Info

Product

Resources

About