CENP-A, -B, and -C Chromatin Complex That Contains the I-Type α-Satellite Array Constitutes the Prekinetochore in HeLa Cells

Ando, Satoshi; Yang, Hua; Nozaki, Naohito; Okazaki, Tuneko; Yoda, Kinya

doi:10.1128/mcb.22.7.2229-2241.2002

Cited by 158 publications

(171 citation statements)

References 62 publications

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“…A. thaliana, AtCENP-C (this work); P. balsamifera, Populus balsamifera EST (BI137512); M. truncatula, Medicago truncatula EST (CB891635); O. sativa, Oryza sativa cDNA clone, full insert sequence (AK073890); L. esculentum, Lycopersicon esculentum EST (AI485238); S. tuberosum, Solanum tuberosum EST (BQ509271); P. coccineus Phaseolus coccineus EST (CA902018); Z. mays, Zea mays CENPCA protein (CenpcA) mRNA (AF129857). found in some alphoid DNA (Masumoto et al 1989), and chromatin immunoprecipitation suggests that CENP-A nucleosomes form a complex with CENP-B and CENP-C through interaction with DNA (Ando et al 2002). Recently, Suzuki et al (2004) also reported that human CENP-C interact with CENP-B and one of the two interaction domains of CENP-C included region I.…”

Section: Resultsmentioning

confidence: 98%

Characterization of a CENP-C homolog in Arabidopsis thaliana

et al. 2004

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Centromere protein C (CENP-C) is a component of the kinetochore essential for correct segregation of sister chromatids in mammals. In Arabidopsis thaliana , a single-copy gene encoding a protein homologous to CENP-C has been found by homology in the whole-genome sequence. To investigate the CENP-C homolog (AtCENP-C), we cloned cDNAs by RT-PCR and determined its full-length coding sequence. Antibodies against the synthetic peptide for the C-terminal residues of AtCENP-C detected a polypeptide in Arabidopsis cell extracts on western blots. Immunofluorescence labeling with the antibodies and fluorescence in situ hybridization demonstrated clearly that AtCENP-C is present at the centromeric regions throughout the cell cycle.

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Section: Resultsmentioning

confidence: 98%

Characterization of a CENP-C homolog in Arabidopsis thaliana

et al. 2004

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“…CENP-N is the only CCAN protein that has been shown to interact directly with CENP-A nucleosomes (44). CENP-C, CENP-T, and CENP-W coimmunoprecipitate with H3 nucleosomes after extensive nuclease digestion (19,45). This suggests that some canonical H3 nucleosomes must be close to CENP-A-rich subdomains in the inner kinetochore.…”

Section: Discussionmentioning

confidence: 99%

A super-resolution map of the vertebrate kinetochore

Ribeiro

Vagnarelli

Yang

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

191

217

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A longstanding question in centromere biology has been the organization of CENP-A-containing chromatin and its implications for kinetochore assembly. Here, we have combined genetic manipulations with deconvolution and super-resolution fluorescence microscopy for a detailed structural analysis of chicken kinetochores. Using fluorescence microscopy with subdiffraction spatial resolution and single molecule sensitivity to map protein localization in kinetochore chromatin unfolded by exposure to a low salt buffer, we observed robust amounts of H3K9me3, but only low levels of H3K4me2, between CENP-A subdomains in unfolded interphase prekinetochores. Constitutive centromere-associated network proteins CENP-C and CENP-H localize within CENP-A-rich subdomains (presumably on H3-containing nucleosomes) whereas CENP-T localizes in interspersed H3-rich blocks. Although interphase prekinetochores are relatively more resistant to unfolding than surrounding pericentromeric heterochromatin, mitotic kinetochores are significantly more stable, reflecting mitotic kinetochore maturation. Loss of CENP-H, CENP-N, or CENP-W had little or no effect on the unfolding of mitotic kinetochores. However, loss of CENP-C caused mitotic kinetochores to unfold to the same extent as their interphase counterparts. Based on our results we propose a new model for inner centromeric chromatin architecture in which chromatin is folded as a layered boustrophedon, with planar sinusoids containing interspersed CENP-A-rich and H3-rich subdomains oriented toward the outer kinetochore. In mitosis, a CENP-C-dependent mechanism crosslinks CENP-A blocks of different layers together, conferring extra stability to the kinetochore.

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“…Such a nucleosome arrangement is postulated to form the platform for kinetochore assembly (Espelin et al 2003;Bloom et al 2006;Westermann et al 2007). In human centromeres, the nucleosomes between two adjacent CENPB boxes are in phase, implying that the CENPA/CENPB nucleosomes over the whole ␣I-type array are orderly positioned, reflecting the repetitive nature of the underlying DNA (Ando et al 2002). Here we report that an array of nucleosomes is orderly positioned in the unique central core sequence of fission yeast centromere.…”

Section: Genome Research 1069mentioning

confidence: 99%

A high-resolution map of nucleosome positioning on a fission yeast centromere

Song¹,

Liu²,

Liu³

et al. 2008

Genome Res.

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A key element for defining the centromere identity is the incorporation of a specific histone H3, CENPA, known as Cnp1p in Schizosaccharomyces pombe. Previous studies have suggested that functional S. pombe centromeres lack regularly positioned nucleosomes and may involve chromatin remodeling as a key step of kinetochore assembly. We used tiling microarrays to show that nucleosomes are, in fact, positioned in regular intervals in the core of centromere 2, providing the first high-resolution map of regional centromere chromatin. Nucleosome locations are not disrupted by mutations in kinetochore protein genes cnp1, mis18, mis12, nuf2, mal2; overexpression of cnp1; or the deletion of ams2, which encodes a GATA-like factor participating in CENPA incorporation. Bioinformatics analysis of the centromere sequence indicates certain enriched motifs in linker regions between nucleosomes and reveals a sequence bias in nucleosome positioning. In addition, sequence analysis of nucleosome-free regions identifies novel binding sites of Ams2p. We conclude that centromeric nucleosome positions are stable and may be derived from the underlying DNA sequence.

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CENP-A, -B, and -C Chromatin Complex That Contains the I-Type α-Satellite Array Constitutes the Prekinetochore in HeLa Cells

Cited by 158 publications

References 62 publications

Characterization of a CENP-C homolog in Arabidopsis thaliana

Characterization of a CENP-C homolog in Arabidopsis thaliana

A super-resolution map of the vertebrate kinetochore

A high-resolution map of nucleosome positioning on a fission yeast centromere

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