Celulas Sanguineas De Schroederichthys Chilensis (Guichenot 1848) (Elasmobranchii, Scyliorhinidae): La Serie Blanca

Abstract

RESUMENLa Ictiohematología es una herramienta de uso común con fines clínicos en peces de importancia económica, con un menor aporte en la comprensión de la fisiología del organismo en relación con su medio. En este contexto y frente a la escasa información publicada de aspectos hematológicos de condrictios, se propuso caracterizar los leucocitos de la pintarroja Schroederichtys chilensis y determinar los valores de referencia cuantitativos. Se obtuvo sangre de pintarrojas capturadas en la Bahía Coliumo (VIII Región, Chile). En extensiones teñidas con May Grünwald y Giemsa se caracterizó los siguientes leucocitos: linfocitos, granulocitos eosinófilos, granulocitos polimorfonucleares heterófilos, monocitos, blastos y trombocitos. Los leucocitos, en general, presentaron características similares a las descritas en otros peces, destacando la elevada presencia de eosinófilos con gránulos prominentes. Cuantitativamente, se encontraron en el siguiente orden: trombocitos (40-50%) > eosinófilos (15-25%) > linfocitos (18-25%) > heterófilos (5-9%) > monocitos (0-1%) = blastos (0 -1%). En comparación con otros peces, la eosinofilia (la más elevada descrita en peces), produce una distorsión de la fórmula leucocitaria, posiblemente por la presencia de trypanosoma humboldti en la sangre. Los parámetros hematológicos informados son un aporte al conocimiento de la especie y servirán como base comparativa para futuras aplicaciones. PALABRAS CLAVES: Schroederichthyes chilensis, Hematología, leucocitos, eosinofilia, tripanosoma, Chile. ABSTRACTIchthyohematology is a common tool in the clinic analysis of economically important fish, and used less to understand fish physiology in relation to the environment. In this context and because of the scarce information about the condrictian hematology, we characterize the leukocytes of the Chilean catshark Schroederichtys chilensis and determine the quantitative reference values. Blood was obtained from fish caught in Coliumo Bay (VIII Región, Chile). Slides were stained with May Grünwald Gimsa. Lymphocytes, eosinophil granulocytes, heterophils granulocytes, monocytes, blasts and thrombocytes were characterized. The leukocytes show similar characteristics to other fish, emphasizing the high number of eosinophils with big granules. Quantitatively the rank order is: thrombocytes (40-50%) > eosinophils (15-25%) > lymphocytes (18-25) > heterophils (5-9%) > monocytes (0-1%) = blasts (0 -1%). Comparing with other fish, the catshark eosinophilia (the highest reported among fishes) produces a distortion in the differential leukocyte count, probably due to the presence of Trypanosomes in the blood. The hematological parameters here reported are important for the species knowledge and will be useful as a comparative base for future studies.

Blood protozoans in elasmobranchs of the family Rajidae from Galicia (NW Spain)

“…The species T. scylli and T. rajae have been found in the anterior intestine of elasmobranchs (Lom & Dyková 1992). T. humboldti Morillas, Nascimento & Khan, 1987, has been reported from the red-spotted catshark Schroederichthys chilensis (Guichenot, 1848) by Valenzuela et al (2003). The type species of the genus Haemogregarina is H. stepanowi Danilewky, 1885, a parasite of Emys orbicularis Linnaeus, 1758.…”

“…A recent study of a trypanosome-infected population of the red-spotted catshark Schroederichthys chilensis (Guichenot, 1848) (infection prevalence 87%) found the highest eosinophil percentage reported to date in fishes (Valenzuela et al 2003).…”

Blood cytology of the common jollytail (Galaxias maculatus) (Jenyns, 1842) (Osmeriformes: Galaxiidae) at postlarval and adult stages

“…The absent of thick grain leukocytes (basophiles and eosinophils) is coincident with other reports that indicate these cells are scarcely present in the periferical blood, furthermore, their absence is usual in most teleosts fish (Olabuenaga 2000, Tavares-Diaz 2006 ). This might be related to the fact that the granulocytes are located in other tissues, such as dermis, intestinal tissue, gills, natatory bladder, hematopoietic tissue, nasal epithelium, heart and inflamed tissue in general (Ellis et al 1981, Suzuki and Iida 1992, Olabuenaga 2000, Valenzuela et al 2003. The granulocytes of thick grain belongs to an heterogeneous group of cells named mastocytes which plays a defensive role and are located in different tissues in the organism (Reite 1998).…”

Hematological changes in Eleginops maclovinus during an experimental Caligus rogercresseyi infestation

“…Studies performed by Valenzuela et al (1999) and Valenzuela et al (2003) have characterized blood cells from wild fish native to Chile. To date there are no studies of the differences in the hematologic parameters between uninfested and infested captive robalo E. maclovinus with C. rogercresseyi.…”