Celsior solution and clinical liver transplantation

Maggi, U.; Caccamo, L.; Gatti, Stefano; Paone, G.; Reggiani, P.; Rossi, G.; Latham, Lea; Vannelli, Alberto; Melada, E.; Brambilla, Roberto; Damilano, I; Trezza, Priscila Maria; Lr, Fassati

doi:10.1016/s0041-1345(99)00866-0

Cited by 36 publications

(21 citation statements)

References 6 publications

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“…Recent solutions using high Na + and low K + ratio were successfully assessed in multi-organ preservation. CEL was recently developed as a multi-organ preservation solution [18][19][20]. The high Na + -low K + version of UW was also evaluated in different preservation conditions [21,22].…”

Section: Introductionmentioning

confidence: 99%

Evidence for a Mitochondrial Impact of Trimetazidine during Cold Ischemia and Reperfusion

et al. 2004

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In organ transplantation, ischemia-reperfusion injury (IRI) has been implicated in delayed graft function (DGF) as well as in short- and long-term complications. Using an autotransplant pig kidney model, changes in renal function and morphology were determined after different periods of cold ischemia in kidneys preserved in the University of Wisconsin solution (UW), high-Na⁺ version of UW (HEH) or Celsior (CEL) a newly developed high-Na⁺ solution, with or without trimetazidine (TMZ). Kidney function was better preserved in CEL, UW and particularly HEH in combination with TMZ, particularly after 48 and 72 h. Mitochondria integrity was improved in TMZ-preserved groups. This study indicates that TMZ is efficiently protective against IRI even after prolonged preservation and in different preservation solutions.

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Section: Introductionmentioning

confidence: 99%

Evidence for a Mitochondrial Impact of Trimetazidine during Cold Ischemia and Reperfusion

et al. 2004

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“…The hypothesis behind the present work was that two clinically used cardioplegic solutions, Celsior (Cs) and histidine-rich solution (HBS), prevent mitochondrial damage after ischemia alone or followed by 30-min reperfusion, as opposed to a control Krebs solution, thus explaining the cardioprotection achieved by both in previous studies [11][12][13]. We also focused our studies on two ischemia periods, 4 and 6 h, to follow possible changes from moderate to severe ischemia and on using both male and female Wistar rats, to assess for the first time if ischemia and I/R would differently affect both genders and if the protection afforded by Cs and HBS would also be gender-dependent.…”

Section: Introductionmentioning

confidence: 95%

“…The Cs is a mixture that contains an inert osmotic barrier impermeant to ions and proteins [9] and some compounds important to decrease cellular swelling and to support muscle contraction by promoting energy production and limiting calcium uptake [10]. Cs was initially developed for cardiac transplantation purposes but its innumerous advantages promoted the use for other organs such as liver, lung, kidney and intestine [11,12]. Nowadays, new cardioplegic solutions are under constant development.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Preservation in Celsior Versus Histidine Buffer Solution During Cardiac Ischemia and Reperfusion

2009

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Various stressful conditions such as ischemia in cold cardioplegic solutions and reperfusion occur during heart transplantation. Since ATP production is essential for the maintenance of contractile activity, mitochondrial function may be a mediator of ischemia and ischemia/reperfusion (I/R) injury. We aimed at testing the ability of two distinct cardioplegic solutions, Celsior (Cs) and Histidine Buffer (HBS), to protect rat heart mitochondria (HM) function during ischemia alone or ischemia followed by reperfusion. A standard Krebs-Henseleit solution (KH) was used as "negative" control. Male and Female Wistar rats were divided into control (Ctrl), reperfusion control (Ctrl_R), ischemia and I/R groups. Ischemia and I/R were divided into three subgroups depending on the cardioplegic solution used (Cs, HBS or KH) and subjected to 4-or 6-h ischemia alone or followed by reperfusion. HM respiration and transmembrane electric potential (Deltapsi) were measured with oxygen and TPP(+)-selective electrodes, respectively. Mitochondrial electron microscopy and detection of protein carbonyl groups content were also performed. After ischemic heart preservation, mitochondrial respiration and Deltapsi were not significantly affected except for the respiratory control ratio (RCR). After I/R, state 3 respiration, RCR and Deltapsi were decreased, especially in HM from male and for complex I substrates (CxI). HM preserved in HBS had less membrane disruption, segregation or disintegration. We conclude that (a) female HM were less sensitive to I/R, (b) CxI was particularly affected by I/R, (c) two cardioplegic solutions tested act in different mitochondrial targets preventing mitochondrial collapse.

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“…Furthermore, peak lipase on postoperative day 1, serum amylase and C-reactive protein were not significantly different between the two groups. Some reports, including the one by Becker et al Celsior, an extracellular, low-viscosity preservation solution originally designed for heart transplantation has also been used for experimental pancreas [23][24][25] and other organ preservation [26][27][28][29][30][31][32]. For whole-pancreas transplantation, the use of Celsior has been controversial [23][24][25].…”

Section: Pancreas Preservation For Pancreas Transplantationmentioning

confidence: 99%

Pancreas preservation for pancreas and islet transplantation

Iwanaga

Sutherland

Harmon

et al. 2008

Current Opinion in Organ Transplantation

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Purpose of review-To summarize advances and limitations in pancreas procurement and preservation for pancreas and islet transplantation, and review advances in islet protection and preservation.Recent findings-Pancreases procured after cardiac death, with in-situ regional organ cooling, have been successfully used for islet transplantation. Colloid-free Celsior and histidinetryptophan-ketoglutarate preservation solutions are comparable to University of Wisconsin solution when used for cold storage before pancreas transplantation. Colloid-free preservation solutions are inferior to University of Wisconsin solution for pancreas preservation prior to islet isolation and transplantation. Clinical reports on pancreas and islet transplants suggest that the two-layer method may not offer significant benefits over cold storage with the University of Wisconsin solution: improved oxygenation may depend on the graft size; benefits in experimental models may not translate to human organs. Improvements in islet yield and quality occurred from pancreases treated with inhibitors of stress-induced apoptosis during procurement, storage, isolation or culture. Pancreas perfusion may be desirable before islet isolation and transplantation and may improve islet yields and quality. Methods for real-time, noninvasive assessment of pancreas quality during preservation have been implemented and objective islet potency assays have been developed and validated. These innovations should contribute to objective evaluation and establishment of improved pancreas preservation and islet isolation strategies.Summary-Cold storage may be adequate for preservation before pancreas transplants, but insufficient when pancreases are processed for islets or when expanded donors are used. Supplementation of cold storage solutions with cytoprotective agents and perfusion may improve pancreas and islet transplant outcomes.

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Celsior solution and clinical liver transplantation

Cited by 36 publications

References 6 publications

Evidence for a Mitochondrial Impact of Trimetazidine during Cold Ischemia and Reperfusion

Evidence for a Mitochondrial Impact of Trimetazidine during Cold Ischemia and Reperfusion

Mitochondrial Preservation in Celsior Versus Histidine Buffer Solution During Cardiac Ischemia and Reperfusion

Pancreas preservation for pancreas and islet transplantation

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