Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 → 3)-linked β- d -glucan oligosaccharides

Fu, Yunbin; Cheng, Likun; Meng, Yanyu; Li, Shuguang; Zhao, Xiaoming; Du, Yuguang; Yin, Heng

doi:10.1016/j.carbpol.2015.08.019

Cited by 23 publications

(7 citation statements)

References 19 publications

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“…β-1,3-Glucans are the main cell wall component.β-1,3-Glucanase is able to hydrolyze these β-1,3-glucans, as a defense against fungal pathogens in plants, as well as to suppress fungal growth in fermentation technology [ 3 , 4 ]. Moreover, β-1,3-glucans can be degraded into (1→3)-linked β- d -glucan oligosaccharides with immunomodulating and antitumor properties by β-1,3-glucanase [ 5 , 6 ]. The exo-β-1,3-glucanase gene encodes an immune-dominant protein as a cell wall remodeler, which is a candidate for immunotherapy and can be used in diagnostic tests [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation of β-1,3-Glucanase-Producing Microorganisms from Poria cocos Cultivation Soil via Molecular Biology

Dou

Zhang

et al. 2018

Molecules

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β-1,3-Glucanase is considered as a useful enzymatic tool for β-1,3-glucan degradation to produce (1→3)-linked β-glucan oligosaccharides with pharmacological activity properties. To validly isolate β-1,3-glucanase-producing microorganisms, the soil of Wolfiporia extensa, considered an environment rich in β-1,3-glucan-degrading microorganisms, was subjected to high throughput sequencing. The results demonstrated that the genera Streptomyces (1.90%) and Arthrobacter (0.78%) belonging to the order Actinomycetales (8.64%) in the phylum Actinobacteria (18.64%) were observed in soil for P. cocos cultivation (FTL1). Actinomycetes were considered as the candidates for isolation of glucan-degrading microorganisms. Out of 58 isolates, only 11 exhibited β-1,3-glucan-degrading activity. The isolate SYBCQL belonging to the genus Kitasatospora with β-1,3-glucan-degrading activity was found and reported for the first time and the isolate SYBC17 displayed the highest yield (1.02 U/mg) among the isolates. To check the β-1,3-glucanase contribution to β-1,3-glucan-degrading activity, two genes, 17-W and 17-Q, encoding β-1,3-glucanase in SYBC17 and one gene QLK1 in SYBCQL were cloned and expressed for verification at the molecular level. Our findings collectively showed that the isolates able to secrete β-1,3-glucanase could be obtained with the assistance of high-throughput sequencing and genes expression analysis. These methods provided technical support for isolating β-1,3-glucanase-producing microorganisms.

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Section: Introductionmentioning

confidence: 99%

Isolation of β-1,3-Glucanase-Producing Microorganisms from Poria cocos Cultivation Soil via Molecular Biology

Dou

Zhang

et al. 2018

Molecules

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“…Apart from this, curdlan has unique gelling properties and thus can be applied to increase elasticity and the overall gel strength ( 13 ). Curdlan can also be used to form edible preservative films ( 14 , 15 ). Recent patents of curdlan are mostly concentrated toward its immunomodulatory functions and improvement of physical properties of foods and drugs in the packaging processes.…”

Section: Recent Patents Of Curdlanmentioning

confidence: 99%

“…According to recent reports, curdlan biofilms with 5% caprolactam have been shown to possess better mechanical strength and other physical properties as compared to other films ( 14 ). Curdlan can form two types of gels based on their thermal properties, viz ., low-set gel and high-set gel ( 15 ). The low-set curdlan gel is produced when the aqueous solution of curdlan is heated up to 60°C and then cooled to 40°C in a thermally reversible process.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic and Industrial Applications of Curdlan With Overview on Its Recent Patents

et al. 2021

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Curdlan is an exopolysaccharide, which is composed of glucose linked with β-(1,3)-glycosidic bond and is produced by bacteria, such as Alcaligenes spp., Agrobacterium spp., Paenibacillus spp., Rhizobium spp., Saccharomyces cerevisiae, Candida spp., and fungal sources like Aureobasidium pullulan, Poria cocos, etc. Curdlan has been utilized in the food and pharmaceutical industries for its prebiotic, viscosifying, and water-holding properties for decades. Recently, the usefulness of curdlan has been further explored by the pharmaceutical industry for its potential therapeutic applications. Curdlan has exhibited immunoregulatory and antitumor activity in preclinical settings. It was observed that curdlan can prevent the proliferation of malarial merozoites in vivo; therefore, it may be considered as a promising therapy for the treatment of end-stage malaria. In addition, curdlan has demonstrated potent antiviral effects against human immunodeficiency virus (HIV) and Aedes aegypti virus. It has been suggested that the virucidal properties of curdlans should be extended further for other deadly viruses, such as severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and the current severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2/COVID-19). The prebiotic property of curdlan would confer beneficial effects on the host by promoting the growth of healthy microbiota in the gut and consequently help to reduce gastrointestinal disorders. Therefore, curdlan can be employed in the manufacture of prebiotics for the management of various gastrointestinal dysbiosis problems. Studies on the mechanism of action of curdlan-induced suppression in microbial and tumor cells at the cellular and molecular levels would not only enhance our understanding regarding the therapeutic effectiveness of curdlan but also help in the discovery of new drugs and dietary supplements. The primary focus of this review is to highlight the therapeutic interventions of curdlan as an anticancer, anti-malaria, antiviral, and antibacterial agent in humans. In addition, our review provides the latest information about the chemistry and biosynthesis of curdlan and its applications for making novel dairy products, functional foods, and nutraceuticals and also details about the recent patents of curdlan and its derivatives.

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“…Alkaline neutralization method was developed as an alternative to promote subsequent hydrolysis. While both pretreatment were effective to some extent, the resulting oligosaccharides varies in degree of polymerization (DP) from 2-10, undesirable for structural and functional studies and for most other applications [11,12]. Microwave-assisted thermal hydrolysis also leads to a mix of oligosaccharides [13].…”

Section: Introductionmentioning

confidence: 99%

A High-Yielding Enzymatic Process for Production of Bioactive β-1,3-Glucan Oligosaccharides with Defined Size

Guan¹,

Yu²,

Liu³

et al. 2018

J Glycobiol

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The study is to develop a bioprocess to depolymerize curdlan for production of β-1,3 oligosaccharides with a uniform size. A codon-optimized Streptomyces laminarinase was expressed in an E. coli system, resulting in an enzyme preparation (19.1 U/L) highly active on solid curdlan substrate without any pre-treatment. In a preparative scale reaction at pH 5.5 and 55°C, 90% conversion and a product concentration close to 27 g/L were achieved within 6 hours. The major product was identified as β3-glucan pentasaccharide based on HPLC, TLC, and MS analyses. A single-enzyme based bioprocess was highly efficient in deriving β3-glucan pentasaccharide from solid curdlan. Enzyme to curdlan ratio and reaction time are critical parameters for high-yield production of β3-glucan pentasaccharide from curdlan.

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Cellulosimicrobium cellulans strain E4-5 enzymatic hydrolysis of curdlan for production of (1 → 3)-linked β- d -glucan oligosaccharides

Cited by 23 publications

References 19 publications

Isolation of β-1,3-Glucanase-Producing Microorganisms from Poria cocos Cultivation Soil via Molecular Biology

Isolation of β-1,3-Glucanase-Producing Microorganisms from Poria cocos Cultivation Soil via Molecular Biology

Therapeutic and Industrial Applications of Curdlan With Overview on Its Recent Patents

A High-Yielding Enzymatic Process for Production of Bioactive β-1,3-Glucan Oligosaccharides with Defined Size

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