Cellulosic Butanol Biorefinery: Production of Biobutanol from High Solid Loadings of Sweet Sorghum Bagasse—Simultaneous Saccharification, Fermentation, and Product Recovery

Qureshi, Nasib; Saha, Badal C.; Liu, S.; Ezeji, Thaddeus Chukwuemeka; Nichols, Nancy N.

doi:10.3390/fermentation7040310

Cited by 7 publications

(8 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analytical-grade standard acetic (Sigma Chemicals) and butyric (Sigma-Aldrich, St. Louis, MO, USA) acids were obtained from these companies. Details of their measurement have been published elsewhere [21,22]. Prior to injecting the samples to the GC, they were diluted by 20 fold using distilled water.…”

Section: Analysesmentioning

confidence: 99%

“…In addition to the application of energy efficient product recovery techniques, the economic potential of this process can only be realized if followings are considered: (i) the use of cost-effective nutrient media and (ii) high-productivity reactors. There are several kinds of nutrient media that can be applied to industrial fermentations in general, including reinforced clostridial medium (RCM), Luria-Bertani medium (LBM) [20], P2 medium [21,22], trypticase soy broth (TSB), and corn steep liquor (CSL) [23][24][25]. RCM, LBM and P2 media are refined and costly, so their application may not be cost-effective for this large-volume/low-value product, thus leaving TSB and CSL as the possible viable options.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Butyric Acid Production by Fermentation: Employing Potential of the Novel Clostridium tyrobutyricum Strain NRRL 67062

2022

Self Cite

View full text Add to dashboard Cite

In this study, the ability of a novel strain of Clostridium tyrobutyricum NRRL 67062 to produce butyric acid during glucose fermentation was evaluated. The strain was evaluated for substrate and product inhibition in batch experiments using anaerobic tubes. To characterize glucose inhibition, initial glucose concentrations ranging from 60 to 250 g L−1 were used, and it was demonstrated that a glucose concentration of 250 g L−1 exerted strong inhibition on cell growth and fermentation. To evaluate butyric acid inhibition, the culture was challenged with 5–50 g L−1 of butyric acid at an initial pH of 6.5. These experiments were performed without pH control. When challenged with a butyric acid concentration of 50 g L−1, cell growth was slow; however, it produced 8.25 g L−1 of butyric acid. This suggested that the butyric acid tolerance of the culture was 58 g L−1. In a scaled-up batch experiment, which was performed in a 2.5 L fermentor with an initial glucose concentration of 100 g L−1, the pH was controlled at 6.5. In this experiment, the strain produced 57.86 g L−1 of butyric acid and 12.88 g L−1 of acetic acid, thus producing 70.74 g L−1 of total acids with a productivity of 0.69 g·L−1·h−1. A concentration of 70.74 g L−1 of acids equates to a yield of 0.71 g of acid per g consumed glucose. The maximum cell concentration was 3.80 g L−1, which may have been the reason for high productivity in the batch culture. Finally, corn steep liquor (CSL; a commercial nutrient solution) provided greater growth and acid production than the refined medium.

show abstract

Section: Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Butyric Acid Production by Fermentation: Employing Potential of the Novel Clostridium tyrobutyricum Strain NRRL 67062

2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…For economic reasons, use of biomass greater than 90 g/L [15] is desirable to reduce the process cost, as it would require smaller size pretreatment and hydrolysis reactors. In some of the pretreatment studies, biomass concentration as high as 250 to 300 (25-30%) g/L has been used [18,19]. Use of such a high biomass concentration can result in the production of 75 g/L xylose and over 100 g/L glucose.…”

Section: Introductionmentioning

confidence: 99%

“…In our long-term continuous bioreactors in which concentrated hydrolyzed mixed sugar solutions were allowed to ferment, some residual xylose remained unfermented. These residual xylose concentrations were in the range of 3.89 to 7.78 g/L [19,20]. This fermentation stream containing xylose sugar cannot be disposed of or released into the environment as it may cause severe pollution problems such as polluting rivers, streams, and air.…”

Section: Introductionmentioning

confidence: 99%

Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There?

et al. 2023

Self Cite

View full text Add to dashboard Cite

This study applied concentrated xylose (60–250 g/L) medium to produce butanol (acetone butanol ethanol, or ABE). A control batch fermentation of 61 g/L initial glucose using Clostridium beijerinckii P260 resulted in a productivity and yield of 0.33 g/L·h and 0.43 g/g, respectively. Use of 60 g/L xylose in a batch system resulted in productivity and yield of 0.26 g/L·h, and 0.40 g/g, respectively. In these two experiments, the culture fermented 89.3% glucose and 83.6% of xylose, respectively. When ABE recovery was coupled with fermentation for continuous solvent removal, the culture fermented all the added xylose (60 g/L). This system resulted in a productivity and yield of 0.66 g/L·h and 0.44 g/g, respectively. When the sugar concentration was further increased above 100 g/L, only a small fraction of the sugar was fermented in batch cultures without product removal. However, with simultaneous product removal, all the xylose (150 g/L) was fermented provided the culture was fed with nutrients intermittently. In this system, 66.32 g/L ABE was produced from 150 g/L xylose with a productivity of 0.44 g/L·h and yield of 0.44 g/g. Using the integrated culture system allowed sugar consumption to be increased by 300% (150 g/L). The continuous system using xylose as a feed did not sustain and after 36 days (864 h) of fermentation, it produced only 2–3 g/L ABE. Rather, the culture became acidogenic and produced 4–5 g/L acids (acetic and butyric). This study suggested that xylose be fermented in batch reactors coupled with simultaneous product recovery rather than in continuous reactors.

show abstract

“…Lignocellulose biomass represents one of the most abundant and promising renewable materials on the planet and, as such, it could serve for sustainable production of bulk chemical commodities via fermentation [ 1 ], including solvents such as acetone–butanol–ethanol (ABE) produced by solventogenic clostridia [ 2 – 4 ]. To reap the indisputable environmental benefits of utilizing lignocellulose biomass, a number of obstacles need to be overcome, including the recalcitrant nature of the material [ 5 , 6 ] and the low sugar content of the resulting hydrolysate due to limitations in the solid-to-liquid phase ratio [ 7 , 8 ]. There are many methods that assist in loosening the structure of tightly interconnected cellulose, hemicellulose and lignin [ 9 ]; biological [ 10 ], physical, chemical [ 11 ] and their various combinations [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation

Branska,

Koppova,

Husakova

et al. 2024

Biotechnol Biofuels

View full text Add to dashboard Cite

Background Inhibitors that are released from lignocellulose biomass during its treatment represent one of the major bottlenecks hindering its massive utilization in the biotechnological production of chemicals. This study demonstrates that negative effect of inhibitors can be mitigated by proper feeding strategy. Both, crude undetoxified lignocellulose hydrolysate and complex medium supplemented with corresponding inhibitors were tested in acetone–butanol–ethanol (ABE) fermentation using Clostridium beijerinckii NRRL B-598 as the producer strain. Results First, it was found that the sensitivity of C. beijerinckii to inhibitors varied with different growth stages, being the most significant during the early acidogenic phase and less pronounced during late acidogenesis and early solventogenesis. Thus, a fed-batch regime with three feeding schemes was tested for toxic hydrolysate (no growth in batch mode was observed). The best results were obtained when the feeding of an otherwise toxic hydrolysate was initiated close to the metabolic switch, resulting in stable and high ABE production. Complete utilization of glucose, and up to 88% of xylose, were obtained. The most abundant inhibitors present in the alkaline wheat straw hydrolysate were ferulic and coumaric acids; both phenolic acids were efficiently detoxified by the intrinsic metabolic activity of clostridia during the early stages of cultivation as well as during the feeding period, thus preventing their accumulation. Finally, the best feeding strategy was verified using a TYA culture medium supplemented with both inhibitors, resulting in 500% increase in butanol titer over control batch cultivation in which inhibitors were added prior to inoculation. Conclusion Properly timed sequential feeding effectively prevented acid-crash and enabled utilization of otherwise toxic substrate. This study unequivocally demonstrates that an appropriate biotechnological process control strategy can fully eliminate the negative effects of lignocellulose-derived inhibitors. Graphical Abstract

show abstract

Cellulosic Butanol Biorefinery: Production of Biobutanol from High Solid Loadings of Sweet Sorghum Bagasse—Simultaneous Saccharification, Fermentation, and Product Recovery

Cited by 7 publications

References 21 publications

Butyric Acid Production by Fermentation: Employing Potential of the Novel Clostridium tyrobutyricum Strain NRRL 67062

Butyric Acid Production by Fermentation: Employing Potential of the Novel Clostridium tyrobutyricum Strain NRRL 67062

Can Xylose Be Fermented to Biofuel Butanol in Continuous Long-Term Reactors: If Not, What Options Are There?

Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation

Contact Info

Product

Resources

About