Cellulosic biomass could help meet California's transportation fuel needs

Wyman, Charles E.; Yang, Bin

doi:10.3733/ca.v063n04p185

Cited by 21 publications

(12 citation statements)

References 33 publications

(32 reference statements)

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“…Cellulose, a structural component of plant biomass, is the most abundant feedstock used for the production of alternative liquid fuels, mainly bioethanol. However, cellulose in terrestrial plants is intertwined with lignin, hemicelluloses and pectin, which require extra energy input as pretreatment for their removal [3,4]. Consequently, due to their high carbohydrate content, high productivity and widespread distribution marine macroalgae (seaweeds) are increasingly gaining prominence as an alternative renewable feedstock for sustainable production of biofuels [5].…”

Section: Introductionmentioning

confidence: 99%

Solid state fermentation (SSF)-derived cellulase for saccharification of the green seaweed Ulva for bioethanol production

et al. 2015

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Section: Introductionmentioning

confidence: 99%

Solid state fermentation (SSF)-derived cellulase for saccharification of the green seaweed Ulva for bioethanol production

et al. 2015

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“…While the decomposition of cellulose and hemicellulose increase the fermentable sugars for cultivation, the liberated in the degradation of lignin are considered microbial inhibitors of fermentation [65] Figure 4. Agriculture crop composed of three major constituents: cellulose, hemicellulose and lignin [66] Producing commercial products through fermentation of lignocelluloses is a multi-step process: It starts with pretreatment of the lignocelluloses to release fermentable simple sugars, then the fermentation of simple sugars by living microorganisms to produce hydrocarbons such as biopolymers or alcohols, and lastly recovery from the fermentation broth of the desired fermentation products, and utilization of the by-products. Although the process steps are described separately, the steps may be integrated to improve production performance [67]…”

Section: Agricultural Biomass As Substratementioning

confidence: 99%

Production of Green Biocellulose Nanofibers Through Utilizing Agricultural Wastes

Al-Abdallah¹

2021

Preprint

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In the present study, the green Biocellulose Nanofibers (BC), a vitally emerging biomaterial, was produced by fermentation of wheat straw (WS), as a widely available agricultural waste, using. Two different fermentation methods were used; Separate Hydrolysis and Fermentation (SHF), and Simultaneous Saccharification and Fermentation (SSF). Different acidic and enzymatic WS pretreatment conditions were used to understand the effect of pretreatment conditions on BC production. Afterward, sugar hydrolsates were simultaneously or separately inoculated with Gluconacetobacter Xylinum bacterium (i.e., for SSF and SHF, respectively), at optimum production conditions in shake flasks for 7 days to produce the biocellulose nanofibers. BC productions of 9.7 g/L in SHF and 10.8 g/L in SSF were achieved when WS was pretreated with dilute acids. Enzymatic treatment of WS after acidic pretreatment increased sugars’ concentrations from the hydrolysis, which increased BC production in SHF to 10.6 g/L. However, enzymes in SSF broke cellulose I alpha linkage in BC and decreased its production compared to no enzymatic treatment. Results show that glucose extracted from WS (~55% of total sugars) was found essential for the cellular metabolism, while xylose (~28% of total sugars) was highly consumed during cells growth phase. Generally, increasing thermal treatment, time and temperature, resulted in increasing furfural concentration. This observed to inhibits bacterial cells growth and leads to lower nanofibers yield when exists at concentration higher than 1 g/L threshold. In general, results obtained in the present study demonstrate the ability of utilizing agricultural wastes in the fermentation production of BC. Such a step is expected to eliminate cost of expensive pure sugars as a carbon source in the fermentation. Also the study shows an improved production yield by using effective fermentation techniques as SSF compared to classical methods used in literature.

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“…Unlike fossil fuel, ethanol is a renewable energy source produced via fermentation of sugars from various biomasses. Nowadays, ethanol is produced from both: food based biomass such as corn/sugarcane/wheat (Shen et al, 2012;Ferreira et al, 2014;Mohagheghi et al, 2015), and non-food based materials such as lignocellulosic biomass (Wyman and Yang, 2009;Zhao et al, 2012;Roberts et al, 2015).…”

Section: Author's Declarationmentioning

confidence: 99%

Bioconversion process of source-separated organic waste for ethanol production

Bekmuradov¹

2021

Preprint

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Production of biofuel such as ethanol from lignocellulosic biomass is a beneficial way to meet sustainability, energy security, and environmental goals. Lignocellulosic biomass such as source-separated organic (SSO) waste is particularly attractive since it is widely available, often at a negative cost, reduce the land depletion from using food-based biomass for ethanol production and reduce the amount of generated waste. Therefore, in order to meet the future fuel demands and cope with increasing volume of municipal waste this study was a first attempt to use SSO as a feedstock for ethanol production. The main objectives of the study were: a) to compare standard and modified celluloseorganic- solvent-based lignocellulosic fractionation (COSLIF) pretreatment of SSO waste for ethanol production in terms of enzyme savings, sugar formation and ethanol yields; b) to produce ethanol from SSO by using modified COSLIF pretreatment and fermentation with two different recombinant strains: Z. mobilis 8b and S. cerevisiae DA2416; and c) to develop experimental kinetic model capable of predicting behavior of batch SSCF on SSO waste with different SSO substrate concentrations using Berkeley Madonna program. Based on the obtained results, it was found that SSO is an excellent feedstock material for ethanol conversion. The efficiency of modified COSLIF pretreatment was improved by 20% compared to standard method using ethanol washing of pretreated SSO samples during the experimental procedures instead of acetone. On average, glucose yield from SSO samples pretreated by modified COSLIF was about 90% compared to 10% for untreated samples. S. cerevisiae DA2416 outperformed Z. mobilis 8b on ethanol yields during the fermentation process, with 0.50 g ethanol/g potential sugar fed on SSO in less than 5 days, with a 96% cellulose conversion, totalling in 150 g/L ethanol produced. A kinetic model with newly integrated values of experimentally defined SSO feedstock constants was proven to predict the ethanol yield accurately with substrate concentration ranges of 20 g/L - 50 g/L. Model prediction at higher substrate concentration (e.g. 100 g/L) deviated from the experimental values, suggesting that ethanol inhibition is a major factor in bioethanol conversion.

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Cellulosic biomass could help meet California's transportation fuel needs

Cited by 21 publications

References 33 publications

Solid state fermentation (SSF)-derived cellulase for saccharification of the green seaweed Ulva for bioethanol production

Solid state fermentation (SSF)-derived cellulase for saccharification of the green seaweed Ulva for bioethanol production

Production of Green Biocellulose Nanofibers Through Utilizing Agricultural Wastes

Bioconversion process of source-separated organic waste for ethanol production

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