Cellulose nanofibers production using a set of recombinant enzymes

Rossi, Bruno Roberto; Pellegrini, Vanessa de Oliveira Arnoldi; Cortez, Anelyse Abreu; Chiromito, Emanoele Maria Santos; Carvalho, Antônio J. F.; Pinto, Lidiane O.; Rezende, Camila A.; Mastelaro, Valmor Roberto; Polikarpov, Igor

doi:10.1016/j.carbpol.2020.117510

Cited by 39 publications

(18 citation statements)

References 65 publications

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“…The mechanical treatment also typically results in low solid density aqueous dispersions of CNFs, leading to transportation and storage difficulties. [133] Current studies have focused on green production of CNF by using chemical or enzymatic pretreatment to reduce energy input requirements. By introducing negative surface charge onto cellulose via TEMPO (2,2,6,6-Tetramethylpiperidinyloxy or 2,2,6,6-Tetramethylpiperidine 1-oxyl) oxidation (Figure 7c), [134] mechanical treatment becomes easier with lower energy consumption.…”

Section: Preparation Of Cnfsmentioning

confidence: 99%

Fiber‐Based Biopolymer Processing as a Route toward Sustainability

Shi

et al. 2021

Advanced Materials

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nomic losses, and a reduction in natural fossil resources. With the current recycling infrastructure, only 14% of current plastic-packaging waste is collected, and even with this recycling, 4% is lost during the process and 8% is redirected for the fabrication of lower-value applications. [5] The long degradation half-lives of synthetic plastics, which break down in the environment over decades or centuries, is the central problem of sustainability. [4,6] Therefore, seeking sustainable and biodegradable alternatives to synthetic plastics have become a pressing task. Developing biodegradable products from renewable resources (e.g., natural biopolymers) represents the best option by fitting material design, production, use, and disposal into a circular materials lifecycle approach (Figure 1b). [7] Instead of losing fossil fuel resources into landfills and causing environmental burdens, natural biopolymers offer options for composting/degradation, thus, recycling the carbon back to environmental cycles for the regeneration of feedstocks. Besides being sustainable and biodegradable, fibrous structural biopolymers from plants (e.g., cellulose) and animals (e.g., silk, chitin) have unique hierarchical structures, with structural integrity, flexibility, and toughness. Therefore, fibrous structural biopolymers represent a class of material that could help to supplement or replace some conventional synthetic plastics. Additionally, the inherent biocompatibility of most natural biopolymers makes them important candidates for added-value applications, such as in regenerative medicine, drug delivery, and tissueimplantable devices. Among these natural biopolymers, cellulose, chitin, and silk fibroin represent the most abundant and investigated biopolymers in the categories of polysaccharides and proteins (Table 1).The first step in this progression toward sustainability is to process fibrous biopolymers into material formats suitable for targeted applications. The sophisticated hierarchical structure of fibrous biopolymers, which consists of well-organized structural features from molecular to nano-to macroscopic length scales, employ extensive hydrogen bonding networks embedded within semicrystalline structures across all structural levels. This structural stability endows fibrous biopolymers with exceptional mechanical properties, but also generates challenges with the direct processing of the fibrous materials into useful material formats via traditional thermal processing methods that are widely used for synthetic polymer processing Some of the most abundant biomass on earth is sequestered in fibrous biopolymers like cellulose, chitin, and silk. These types of natural materials offer unique and striking mechanical and functional features that have driven strong interest in their utility for a range of applications, while also matching environmental sustainability needs. However, these material systems are challenging to process in cost-competitive ways to compete with synthetic plastics due to the limited options for ther...

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Section: Preparation Of Cnfsmentioning

confidence: 99%

Fiber‐Based Biopolymer Processing as a Route toward Sustainability

Shi

et al. 2021

Advanced Materials

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“…Chemical pretreatment can destroy hydrogen bonds network inside the fibers, and the rigid structures of the fibers are softened, so that the fibers can be easily homogenized using a high pressure homogenizer. [ 114‐118 ] The most commonly used chemical pretreatment method is TEMPO (2,2,6,6‐Tetramethylpiperidine 1‐oxyl) oxidation. In the presence of NaBr and NaOCl, TEMPO oxidation is highly selective, which can oxidize hydroxyl groups on cellulose glucose unit C6 to carboxyl groups, while the hydroxyl groups on cellulose glucose unit C2 and C3 are not oxidized.…”

Section: Preparation Of Mofs Cellulose Derivatives and Mof@cellulose Hybridsmentioning

confidence: 99%

“…Since the rigid structure of the fibers has been softened, there is little blockage during the homogenization process. [ 114‐118 ] Moreover, the most common method to produce CNFs is mechanical techniques. [ 119‐120 ] These techniques pave the way for the industrial production of CNFs.…”

Section: Preparation Of Mofs Cellulose Derivatives and Mof@cellulose Hybridsmentioning

confidence: 99%

Recent Progress in Metal‐Organic Frameworks@Cellulose Hybrids and Their Applications

et al. 2021

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In recent years, metal‐organic frameworks (MOFs) are attracting increasing attention due to their charming properties. However, the intrinsic fragility, powdered crystalline state, and poor processibility of MOFs hinder their further application. By this, the designs of MOF‐based aerogels, hydrogels, membranes are effective ways to solve this problem. Graphene, silica, synthetic polymer and cellulose are the commonly used raw materials for fabricating MOF‐based aerogels, hydrogels, and membranes. Among them, cellulose and its derivatives are the best candidates to support the powdered MOFs from the perspectives of economy, environmental protection and property. This review focuses on discussing the advantages of MOF@cellulose hybrids in applications such as water treatment, antibacterial, gas separation and adsorption, energy storage, drug delivery, catalysis, and other smart composites. MOF@cellulose‐based aerogels, hydrogels and membranes can provide valuable guidance for investigating the practical application of MOFs in terms of processability, reusability, stability and easiness in handling.

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“…The importance of mechanistic understanding of LPMO-driven catalysis and light activation extrapolates the level of biofuels and could be applied, for example, to the production of cellulose nanoparticles and nanofibrils. These materials, which could be obtained from plant biomass, are eco-friendly and biodegradable and can be used in various areas such as food packaging, biomedicine, electronics, and cosmetics, replacing materials from nonrenewable sources (Rossi et al, 2021). Thus, by better mechanistic understanding and consequent optimization of the LPMOs activities and applications, one can contribute to building bioeconomy and a greener, more sustainable society.…”

Section: Practical Implications Of the Present Studymentioning

confidence: 99%

Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A

et al. 2021

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Cost-efficient plant biomass conversion using biochemical and/or chemical routes is essential for transitioning to sustainable chemical technologies and renewable biofuels. Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that make part of modern hydrolytic cocktails destined for plant biomass degradation. Here, we characterized MtLPMO9A from Thermothelomyces thermophilus M77 (formerly Myceliophthora thermophila) and demonstrated that it could be efficiently driven by chlorophyllin excited by light in the presence of a reductant agent. However, in the absence of chemical reductant, chlorophyllin and light alone do not lead to a significant release of the reaction products by the LPMO, indicating a low capacity of MtLPMO9A reduction (either via direct electron transfer or via superoxide ion, O2•-). We showed that photocatalysis could significantly increase the LPMO activity against highly crystalline and recalcitrant cellulosic substrates, which are poorly degraded in the absence of chlorophyllin and light. We also evaluated the use of co-substrates by MtLPMO9A, revealing that the enzyme can use both hydrogen peroxide (H2O2) and molecular oxygen (O2) as co-substrates for cellulose catalytic oxidation.

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Cellulose nanofibers production using a set of recombinant enzymes

Cited by 39 publications

References 65 publications

Fiber‐Based Biopolymer Processing as a Route toward Sustainability

Fiber‐Based Biopolymer Processing as a Route toward Sustainability

Recent Progress in Metal‐Organic Frameworks@Cellulose Hybrids and Their Applications

Combining pieces: a thorough analysis of light activation boosting power and co-substrate preferences for the catalytic efficiency of lytic polysaccharide monooxygenase MtLPMO9A

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