Cellulose nanocrystals of variable sulfation degrees can sequester specific platelet lysate-derived biomolecules to modulate stem cell response

Mendes, Bárbara B.; Gomez‐Florit, Manuel; Osório, Hugo; Vilaça, Adriana; Domingues, Rui M. A.; Reis, Rui L.; Gomes, Manuela E.

doi:10.1039/d0cc01850c

Cited by 10 publications

(14 citation statements)

References 31 publications

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“…The typical CNC production method renders nanoparticles that are grafted with sulfate ester groups on their surface (about 292 ± 5 mmol kg −1 , obtained from ref. [17] ), imparting a negative surface charge (−63.03 ± 0.41 mV), in agreement with the literature. [27] In addition, like other nanocelluloses, CNC have an abundance of hydroxyl groups on their surface that are amenable to hydrogen bonding.…”

Section: Biofunctionalization Of Anisotropic Surfaces By Sequesteringsupporting

confidence: 91%

“…Although this increase indicates the release of weakly adsorbed PL proteins from the CNC-coated surfaces, [36] around 25% of the total mass before the PBS washing, the results confirm the presence of strongly adsorbed PL biomolecules on CNC after the PBS washing, in agreement with our previous results using CNC suspensions. [17] Overall, these results suggest that CNC can effectively mediate the sequestration of PL proteins on Ti/CNC surfaces. These findings are of significant interest because it represents a straightforward strategy to incorporate bioactivity on the surfaces of metallic implant because it can emulate the role of the ECM on the regulation of PL signaling biomolecules.…”

Section: Biofunctionalization Of Anisotropic Surfaces By Sequesteringmentioning

confidence: 73%

“…[30] The produced CNC exhibited the characteristic rod-shaped morphology (Figure S1, Supporting Information), with an average length of 188 ± 69 nm and height of 4 ± 2 nm, in agreement with previous works. [17,31] Leveraging on the rod morphology of CNC, we aimed to develop metallic surfaces showing anisotropic and isotropic bioinstructive nanopatterns to guide cell growth and lead to a superior and more predictable soft tissue integration. Inspired by strategies reported elsewhere, [15,16] we used spin-coating to align CNC and create nanopatterned surfaces.…”

Section: Controlling the Orientation Of Cnc To Produce Anisotropic Namentioning

confidence: 99%

“…Characterization of Nanocoated Surfaces-Proteomic Analysis: To study the proteins absorbed on the CNC, mass spectrometry-based proteomic analysis was performed as previously reported. [17] Briefly, equal volumes of CNC solutions diluted with ultrapure water to a final concentration of 2 mg mL −1 (containing 3.8 mg of CNC) and PL (containing 128.44 mg of total protein content) were incubated for 60 min at 37°C under constant agitation (200 rpm). CNC were separated from the supernatant by centrifugation at 15 000 × g for 30 min.…”

Section: Nanocoating Of the Nanopatterned Surfaces With Platelet Lysatementioning

confidence: 99%

“…We previously showed that CNC possess a versatile surface chemistry able to sequester in their protein corona biomolecules from platelet lysate (PL), [ 17 ] a milieu of biological elements (growth factors, cytokines, antimicrobial peptides, and scaffolding proteins) known to modulate different cell functions, including adhesion, proliferation, and differentiation. [ 18 ] Actually, the use of platelet derived products, such as platelet‐rich‐plasma (PRP) and PL, is gaining increasing attention in clinical settings.…”

Section: Introductionmentioning

confidence: 99%

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Multifunctional Surfaces for Improving Soft Tissue Integration

Vilaça

Domingues

Tiainen

et al. 2021

Adv Healthcare Materials

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Metallic implants are widely used in diverse clinical applications to aid in recovery from lesions or to replace native hard tissues. However, the lack of integration of metallic surfaces with soft tissue interfaces causes the occurrence of biomaterial-associated infections, which can trigger a complicated inflammatory response and, ultimately, implant failure. Here, a multifunctional implant surface showing nanoscale anisotropy, based on the controlled deposition of cellulose nanocrystals (CNC), and biological activity derived from platelet lysate (PL) biomolecules sequestered and presented on CNC surface, is proposed. The anisotropic radial nanopatterns are produced on polished titanium surfaces by spin-coating CNC at high speed. Furthermore, CNC surface chemistry allows to further sequester and form a coating of bioactive molecules derived from PL. The surface anisotropy provided by CNC guides fibroblasts growth and alignment up to 14 days of culture. Moreover, PL-derived biomolecules polarize macrophages toward the M2-like anti-inflammatory phenotype. These results suggest that the developed multifunctional surfaces can promote soft tissue integration to metallic implants and, at the same time, prevent bacterial invasion, tissue inflammation, and failure of biomedical metallic implants.

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Section: Biofunctionalization Of Anisotropic Surfaces By Sequesteringsupporting

confidence: 91%

Section: Biofunctionalization Of Anisotropic Surfaces By Sequesteringmentioning

confidence: 73%

Section: Controlling the Orientation Of Cnc To Produce Anisotropic Namentioning

confidence: 99%

Section: Nanocoating Of the Nanopatterned Surfaces With Platelet Lysatementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multifunctional Surfaces for Improving Soft Tissue Integration

Vilaça

Domingues

Tiainen

et al. 2021

Adv Healthcare Materials

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3D Bioprinting of Miniaturized Tissues Embedded in Self‐Assembled Nanoparticle‐Based Fibrillar Platforms

Bakht

Gomez‐Florit

Lamers

et al. 2021

Adv Funct Materials

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The creation of microphysiological systems like tissue and organ-on-chip for in vitro modeling of human physiology and diseases is gathering increasing interest. However, the platforms used to build these systems have limitations concerning implementation, automation, and cost-effectiveness. Moreover, their typical plastic-based housing materials are poor recreations of native tissue extracellular matrix (ECM) and barriers. Here, the controlled selfassembly of plant-derived cellulose nanocrystals (CNC) is combined with the concept of 3D bioprinting in suspension baths for the direct biofabrication of microphysiological systems embedded within an ECM mimetic fibrillar support material. The developed support CNC fluid gel allows exceptionally highresolution bioprinting of 3D constructs with arbitrary geometries and low restrictions of bioink choice. The further induction of CNC self-assembly with biocompatible calcium ions results in a transparent biomimetic nanoscaled fibrillar matrix that allows hosting different compartmentalized cell types and perfusable channels, has tailored permeability for biomacromolecules diffusion and cellular crosstalk, and holds structural stability to support long-term in vitro cell maturation. In summary, this xeno-free nanoscale CNC fibrillar matrix allows the biofabrication of hierarchical living constructs, opening new opportunities not only for developing physiologically relevant 3D in vitro models but also for a wide range of applications in regenerative medicine.

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The impact of cellulose nanocrystals on the properties of poly(2‐hydroxyethyl methacrylate)‐based contact lenses

Blanc,

Faran,

Dufresne

et al. 2024

J of Applied Polymer Sci

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Contact lenses are widely used corrective devices for addressing vision impairments. In addition to their traditional role, these lenses are increasingly applied in personalized care, biosensing, and drug release, thus broadening their application as multifunctional devices. Nanoparticles are often used to introduce new functionalities to these materials. In this context, the role played by different nanoparticles requires attention to ensure that lens manufacture and patient comfort meet the desired criteria. This study investigates the properties of contact lenses prepared by incorporating cellulose nanocrystals into the poly(2‐hydroxyethyl methacrylate) matrix. Using photopolymerization reactions, polyHEMA‐based lenses and nanocomposites containing freeze‐dried and never‐dried nanoparticles were obtained. Fundamental properties of these lenses were evaluated to verify whether the nanoparticles could have a negative effect on the materials. Subsequently, the potential impact of these nanoparticles on the adsorption of model amino acids was investigated. Our findings reveal that the presence of cellulose nanocrystals modifies on lens thickness, light transmittance, mechanical properties, refractive index (RI), and the uptake of amino acids by the contact lenses. This could potentially affect patient comfort and modify the uptake and release of pharmaceutical ingredients by the material. Additionally, it could impact how these materials interact with biomolecules present in the lacrimal fluid.

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Cellulose nanocrystals of variable sulfation degrees can sequester specific platelet lysate-derived biomolecules to modulate stem cell response

Abstract: Cellulose nanocrystals can bind different patterns of platelet lysate-derived protein in a surface sulfation dependent manner. The potential to direct stem cell fate by solid-phase presentation of defined protein coronas is demonstrated.

Cited by 10 publications

References 31 publications

Multifunctional Surfaces for Improving Soft Tissue Integration

Multifunctional Surfaces for Improving Soft Tissue Integration

3D Bioprinting of Miniaturized Tissues Embedded in Self‐Assembled Nanoparticle‐Based Fibrillar Platforms

The impact of cellulose nanocrystals on the properties of poly(2‐hydroxyethyl methacrylate)‐based contact lenses

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