Cellulose nanocrystals decorated with gold nanoparticles immobilizing GOx enzyme for non-invasive biosensing of human salivary glucose

Saeed, Ayman Ali; Abbas, Mohammed Nooredeen; Singh, Balwinder; Abou-Zeid, Ragab E.; Kamel, Samir

doi:10.1039/c9ay02176k

Cited by 31 publications

(12 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CNC have desirable physical, chemical, and mechanical properties, such as an elastic modulus around 150 GPa, which is greater than the elastic modulus of glass fibers (85 GPa) and aramid (65 GPa) [ 3 , 4 , 5 ], high tensile strength (7500 MPa), high stiffness (Young’s modulus ≥140 GPa), a high surface area, high aspect ratio, an abundance of surface hydroxyl groups available for chemical functionalization, and full biodegradability, due to its natural origin [ 1 , 2 , 3 , 4 , 6 ]. Therefore, CNC have been applied in diverse fields such as medicine [ 2 ], catalysis [ 7 ], biosensing [ 8 ], and as reinforcing materials to improve the mechanical properties of composites in multiple applications, such as plastics, ceramics, concrete, etc. [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Optimization of the Obtaining of Cellulose Nanocrystals from Agave tequilana Weber Var. Azul Bagasse by Acid Hydrolysis

et al. 2021

View full text Add to dashboard Cite

A multilevel factorial design of 23 with 12 experiments was developed for the preparation of cellulose nanocrystals (CNC) from Agave tequilana Weber var. Azul bagasse, an agro-industrial waste from tequila production. The studied parameters were acid type (H2SO4 and HCl), acid concentration (60 and 65 wt% for H2SO4, 2 and 8N for HCl) temperature (40 and 60 °C for H2SO4, 50 and 90 °C for HCl), and hydrolysis time (40, 55 and 70 min for H2SO4; and 30, 115 and 200 min for HCl). The obtained CNC were physical and chemically characterized using dynamic light scattering (DLS), atomic force microscopy (AFM), Fourier-transform infrared spectroscopy (FT-IR), X-ray photoelectron spectroscopy (XPS), and X-ray diffraction (XDR) techniques. The maximum CNC yield was 90 and 96% for HCL and H2SO4, respectively, and the crystallinity values ranged from 88–91%. The size and morphology of A. tequilana CNC strongly depends on the acid type and hydrolysis time. The shortest CNC obtained with H2SO4 (65 wt%, 40 °C, and 70 min) had a length of 137 ± 68 nm, width 33 ± 7 nm, and height 9.1 nm, whereas the shortest CNC obtained with HCl (2 N, 50 °C and 30 min) had a length of 216 ± 73 nm, width 69 ± 17 nm, and height 8.9 nm. In general, the obtained CNC had an ellipsoidal shape, whereas CNC prepared from H2SO4 were shorter and thinner than those obtained with HCl. The total sulfate group content of CNC obtained with H2SO4 increased with time, temperature, and acid concentration, exhibiting an exponential behavior of .

show abstract

Section: Introductionmentioning

confidence: 99%

Optimization of the Obtaining of Cellulose Nanocrystals from Agave tequilana Weber Var. Azul Bagasse by Acid Hydrolysis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Recently, Schyrr et al have demonstrated CNC-and PVA-based platform for use in fluorescence-based biomedical applications 67 and Incani et al have also developed glucose sensors by immobilization of glucose oxidase (GOx) on the cellulose nanocrystal PEI/AuNPs composite 60 . Similarly, Saeed et al proposed a technique with gold nanoparticle decorated functionalized cellulose nanocrystal and immobilized GOx enzyme to sense salivary glucose 68 . Efforts were made for developing point of care biosensors by using conjugation of peptides and protein to CNCs 64,65 .…”

mentioning

confidence: 99%

Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer

Hazra

Kale²,

Aland³

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Herein we report a hierarchically organized, water-dispersible ‘nanocage’ composed of cellulose nanocrystals (CNCs), which are magnetically powered by iron oxide (Fe3O4) nanoparticles (NPs) to capture circulating tumor cells (CTCs) in blood for head and neck cancer (HNC) patients. Capturing CTCs from peripheral blood is extremely challenging due to their low abundance and its account is clinically validated in progression-free survival of patients with HNC. Engaging multiple hydroxyl groups along the molecular backbone of CNC, we co-ordinated Fe3O4 NPs onto CNC scaffold, which was further modified by conjugation with a protein - transferrin (Tf) for targeted capture of CTCs. Owing to the presence of Fe3O4 nanoparticles, these nanocages were magnetic in nature, and CTCs could be captured under the influence of a magnetic field. Tf-CNC-based nanocages were evaluated using HNC patients’ blood sample and compared for the CTC capturing efficiency with clinically relevant Oncoviu platform. Conclusively, we observed that CNC-derived nanocages efficiently isolated CTCs from patient’s blood at 85% of cell capture efficiency to that of the standard platform. Capture efficiency was found to vary with the concentration of Tf and Fe3O4 nanoparticles immobilized onto the CNC scaffold. We envision that, Tf-CNC platform has immense connotation in ‘liquid biopsy’ for isolation and enumeration of CTCs for early detection of metastasis in cancer.

show abstract

“…Thus, the modification of cellulose for the immobilization of enzymes is an important role in amperometric sensors [121,122]. Saeed et al [123] developed recently a non-invasive biosensor for human salivary glucose using a dialdehyde derivative of cellulose nanocrystals (CNCs) immobilized with gold nanoparticles (AuNPs) via an aminothiophenol crosslinker and glucose oxidase (GOx) enzymes via thioctic acid as a double crosslinker. The gold nanoparticles were attached to the cellulose via thiol chemistry.…”

Section: Cellulose-bbased Electrochemical Sensorsmentioning

confidence: 99%

Recent Advances in Cellulose-Based Biosensors for Medical Diagnosis

2020

Self Cite

View full text Add to dashboard Cite

Cellulose has attracted much interest, particularly in medical applications such as advanced biosensing devices. Cellulose could provide biosensors with enhanced biocompatibility, biodegradability and non-toxicity, which could be useful for biosensors. Thus, they play a significant role in environmental monitoring, medical diagnostic tools, forensic science, and foodstuff processing safety applications. This review summarizes the recent developments in cellulose-based biosensors targeting the molecular design principles toward medical detection purposes. The recognition/detection mechanisms of cellulose-based biosensors demonstrate two major classes of measurable signal generation, including optical and electrochemical cellulosic biosensors. As a result of their simplicity, high sensitivity, and low cost, cellulose-based optical biosensors are particularly of great interest for including label-free and label-driven (fluorescent and colorimetric) biosensors. There have been numerous types of cellulose substrates employed in biosensors, including several cellulose derivatives, nano-cellulose, bacterial cellulose, paper, gauzes, and hydrogels. These kinds of cellulose-based biosensors were discussed according to their preparation procedures and detection principle. Cellulose and its derivatives with their distinctive chemical structure have demonstrated to be versatile materials, affording a high-quality platform for accomplishing the immobilization process of biologically active molecules into biosensors. Cellulose-based biosensors exhibit a variety of desirable characteristics, such as sensitivity, accuracy, convenience, quick response, and low-cost. For instance, cellulose paper-based biosensors are characterized as being low-cost and easy to operate, while nano-cellulose biosensors are characterized as having a good dispersion, high absorbance capacity, and large surface area. Cellulose and its derivatives have been promising materials in biosensors which could be employed to monitor various bio-molecules, such as urea, glucose, cell, amino acid, protein, lactate, hydroquinone, gene, and cholesterol. The future interest will focus on the design and construction of multifunctional, miniaturized, low-cost, environmentally friendly, and integrated biosensors. Thus, the production of cellulose-based biosensors is very important.

show abstract

Cellulose nanocrystals decorated with gold nanoparticles immobilizing GOx enzyme for non-invasive biosensing of human salivary glucose

Abstract: A cellulose nanocrystal (CNC) material was converted into its dialdehyde before being decorated with gold nanoparticles (AuNPs) through an aminothiophenol linker.

Cited by 31 publications

References 57 publications

Optimization of the Obtaining of Cellulose Nanocrystals from Agave tequilana Weber Var. Azul Bagasse by Acid Hydrolysis

Optimization of the Obtaining of Cellulose Nanocrystals from Agave tequilana Weber Var. Azul Bagasse by Acid Hydrolysis

Cellulose Mediated Transferrin Nanocages for Enumeration of Circulating Tumor Cells for Head and Neck Cancer

Recent Advances in Cellulose-Based Biosensors for Medical Diagnosis

Contact Info

Product

Resources

About