Cellulose as a Matrix for Nucleic Acid Purification

Su, Xing; Comeau, Anne Marie

doi:10.1006/abio.1998.2987

Cited by 22 publications

(23 citation statements)

References 9 publications

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“…However, we noted that the control, unmodified Whatman No.1 paper, consistently resulted in strong amplification ( S1B Fig ). The ability of cellulose-based paper to entrap or adsorb DNA under specific conditions has been extensively reported, but its use has been limited to storage or transport and not for nucleic acid purification purposes under nonprecipitating conditions [ 10 , 11 , 23 – 25 ]. We further examined the efficiency at which Whatman No.1 can capture DNA and retain it during a brief (one minute) wash prior to DNA amplification directly from the paper.…”

Section: Resultsmentioning

confidence: 99%

“…The use of cellulose for DNA purification is commercially available and is claimed to have improved performance over silica-based DNA purification methods [ 58 , 59 ]. DNA purification using cellulose has been previously reported to be achieved by coaggregating or adsorbing the DNA to the cellulose in the presence of various chemicals, including chaotropic salts [ 60 ], ethanol [ 23 , 61 ], and high salt concentrations and/or crowding agents such as polyethylene glycol [ 11 , 62 , 63 ], which destabilise the DNA structure and facilitate its interaction with the cellulose fibres. In these systems, water or a low salt solution is then required to elute the DNA from the cellulose.…”

Section: Discussionmentioning

confidence: 99%

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Nucleic acid purification from plants, animals and microbes in under 30 seconds

et al. 2017

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Nucleic acid amplification is a powerful molecular biology tool, although its use outside the modern laboratory environment is limited due to the relatively cumbersome methods required to extract nucleic acids from biological samples. To address this issue, we investigated a variety of materials for their suitability for nucleic acid capture and purification. We report here that untreated cellulose-based paper can rapidly capture nucleic acids within seconds and retain them during a single washing step, while contaminants present in complex biological samples are quickly removed. Building on this knowledge, we have successfully created an equipment-free nucleic acid extraction dipstick methodology that can obtain amplification-ready DNA and RNA from plants, animals, and microbes from difficult biological samples such as blood and leaves from adult trees in less than 30 seconds. The simplicity and speed of this method as well as the low cost and availability of suitable materials (e.g., common paper towelling), means that nucleic acid extraction is now more accessible and affordable for researchers and the broader community. Furthermore, when combined with recent advancements in isothermal amplification and naked eye DNA visualization techniques, the dipstick extraction technology makes performing molecular diagnostic assays achievable in limited resource settings including university and high school classrooms, field-based environments, and developing countries.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Nucleic acid purification from plants, animals and microbes in under 30 seconds

et al. 2017

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“…Here we test a new approach to plant DNA extraction using MagnaCel paramagnetic cellulose particles (PMC) integrated with the Maxwell 16 robotic instrument (Promega Corporation, Madison, Wisconsin, USA) (adapted from Mandrekar et al, 2010). These particular cellulose particles have a high DNA‐binding capacity (Su and Comeau, 1999), which Promega asserts is greater than silica. We compared the DNA yield and purity across a wide range of flowering plants among PMC, the silica‐based DNeasy Plant Mini kit (QIAGEN, Dusseldorf, Germany), and a CTAB‐based method (adapted from Doyle and Doyle, 1987).…”

mentioning

confidence: 99%

Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa

et al. 2014

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• Premise of the study: The chemical diversity of land plants ensures that no single DNA isolation method results in high yield and purity with little effort for all species. Here we evaluate a new technique originally developed for forensic science, based on MagnaCel paramagnetic cellulose particles (PMC), to determine its efficacy in extracting DNA from 25 plant species representing 21 families and 15 orders.• Methods and Results: Yield and purity of DNA isolated by PMC, DNeasy Plant Mini Kit (silica column), and cetyltrimethylammonium bromide (CTAB) methods were compared among four individuals for each of 25 plant species. PMC gave a twofold advantage in average yield, and the relative advantage of the PMC method was greatest for samples with the lowest DNA yields. PMC also produced more consistent sample purity based on absorbance ratios at 260:280 and 260:230 nm.• Conclusions: PMC technology is a promising alternative for plant DNA isolation.

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“…We have also developed a nucleic acid extraction module using a solid phase purification method (18) and integrated it into our BPU device. Extraction generally includes releasing the nucleic acid from the sample (lysis), removing contaminants (purification) and recovering the nucleic acid.…”

Section: Nucleic Acid Purification and Concentrationmentioning

confidence: 99%

A miniature integrated device for automated multistep genetic assays

Anderson¹,

Su²,

Bogdan³

et al. 2000

Nucleic Acids Research

204

101

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A highly integrated monolithic device was developed that automatically carries out a complex series of molecular processes on multiple samples. The device is capable of extracting and concentrating nucleic acids from milliliter aqueous samples and performing microliter chemical amplification, serial enzymatic reactions, metering, mixing and nucleic acid hybridization. The device, which is smaller than a credit card, can manipulate over 10 reagents in more than 60 sequential operations and was tested for the detection of mutations in a 1.6 kb region of the HIV genome from serum samples containing as few as 500 copies of the RNA. The elements in this device are readily linked into complex, flexible and highly parallel analysis networks for high throughput sample preparation or, conversely, for low cost portable DNA analysis instruments in point-of-care medical diagnostics, environmental testing and defensive biological agent detection.

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Cellulose as a Matrix for Nucleic Acid Purification

Cited by 22 publications

References 9 publications

Nucleic acid purification from plants, animals and microbes in under 30 seconds

Nucleic acid purification from plants, animals and microbes in under 30 seconds

Paramagnetic cellulose DNA isolation improves DNA yield and quality among diverse plant taxa

A miniature integrated device for automated multistep genetic assays

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