Cellulomonas dongxiuzhuiae sp. nov., Cellulomonas wangleii sp. nov. and Cellulomonas fengjieae sp. nov., isolated from the intestinal contents of Marmota himalayana

Abstract

Six Gram-stain-positive, aerobic or facultative anaerobic, catalase-positive, urease- and oxidase-negative, rod-shaped bacteria (zg-ZUI157T/zg-ZUI40, zg-ZUI222T/zg-ZUI199 and zg-ZUI188T/ zg-ZUI168) were characterized by a polyphasic approach. Optimal growth of the six strains was observed at pH 7.0 and 28 °C. Phylogenetic analyses based on the 16S rRNA gene and 247 core genes revealed that they belong to genus Cellulomonas . The three type strains have low digital DNA-DNA hybridization (19.3–30.1%) and average nucleotide identity values (78.0-85.5%) with all available genomes in the genus Cellulomonas , and a DNA G+C content range of 73.0-74.6 mol%. The major fatty acids detected in strain pairs zg-ZUI157T/zg-ZUI40 and zg-ZUI 222T/zg-ZUI199 were C16:0, anteiso-C15:0 and anteiso A-C15:1, and C16:0, anteiso-C15:0, anteiso A-C15:1 and anteiso-C17:0 in strain pair zg-ZUI188T/zg-ZUI168. Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides were the major polar lipids detected in the three novel species. MK-9(H4) was the predominant quinone detected in strains zg-ZUI222T (87.4 %) and zg-ZUI188T (91.4 %), and MK-9(H4) (49.1 %) and MK-8 (43.4 %) in strain zg-ZUI157T. The cell-wall sugars detected in the three novel species mainly contained rhamnose. The cell-wall peptidoglycan type of the three novel species was A4β, with an inferred l-Orn–d-Asp interpeptide bridge for strains zg-ZUI157T and zg-ZUI222T, and l-Orn–d-Glu for strain zg-ZUI188T. Based on the results of the phenotypic, phylogenetic, genomic hybridization, average nucleotide identity and chemotaxonomic analyses, the six strains should be classified as belonging to three novel Cellulomonas species, for which the names Cellulomonas dongxiuzhuiae sp. nov. (zg-ZUI157T=GDMCC 1.2559T=KCTC 49678T), Cellulomonas wangleii sp. nov. (zg-ZUI222T=GDMCC 1.2501T=KCTC 49675T) and Cellulomonas fengjieae sp. nov. (zg-ZUI188T=GDMCC 1.2563T=KCTC 49674T) are proposed.

Identification, characteristics and rice growth promotion of a highly efficient cellulolytic bacterial strain, Cellulomonas iranensis ZJW-6, isolated from paddy soil in central China

“…Cellulomonas species, unique cellulose degraders, Gram-positive bacteria’s with high GC content, were not commercially significant until recently, as notions of biotransformation of cellulosic agricultural wastes arose ( Dahal et al, 2022 ; Gui Zhang et al, 2022 ; Siriatcharanon et al, 2022 ). Cellulomonas fimi , in particular, is one of the few facultative anaerobes identified to date that is capable of degrading cellulose at oxygen levels as low as 1%, which was thought to be more favorable than aerobic cellulose-degrading fungi in this regard ( Christopherson et al, 2013 ).…”

Cellulomonas xiejunii sp. nov., Cellulomonas chengniuliangii sp. nov. and Cellulomonas wangsupingiae sp. nov., three cellulolytic bacteria isolated from intestinal contents of Marmota himalayana

“…Strains zg-B89 T , zg-Y338 T and zg-Y908 T had similar polar lipid profiles to the closely related species with some small differences in compositions () [4, 16, 40, 41]. All three novel type strains had diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside in common, with a varying number of unidentified phospholipids and unknown phosphoglycolipid (Fig.…”

“…Samples of intestinal contents of M. himalayana were collected from Nangqian County (32° 38′ 26″ N 96° 5′ 25″ E), Yushu Tibetan Autonomous Prefecture of Qinghai Province in 2018. The M. himalayana specimens were aseptically collected and processed as described previously [16]. After the diluted samples were incubated aerobically on brain heart infusion agar supplemented with 5 % (v/v) defibrillated sheep blood (BHIB) or Luria-Bertani (LB) agar at 28 °C, colonies growing on the plates were selected based on morphology diversity.…”

“… Cellulomonas species were mostly isolated from environmental samples, such as various soils, air, sea water, and plants [4–14], and a few members were isolated from animals. During a previous study of bacterial biodiversity in wild animals living on the Qinghai–Tibet Plateau, four Cellulomonas species of wild animal origin were identified [15, 16]. Here, we characterize another six isolates (strains zg-B89 T , zg-B12, zg-Y338 T , zg-Y138, zg-Y908 T and zg-Y766) isolated from intestinal contents of Marmota himalayana using a polyphasic taxonomic approach.…”