Cellulolytic enzymes production guided by morphology engineering

Buffo, Mariane Molina; Ferreira, André Luís Zozzi; Almeida, Renata Maria Rosas Garcia; Farinas, Cristiane S.; Badino, Alberto Colli; Ximenes, Eduardo; Ladisch, Michael R.

doi:10.1016/j.enzmictec.2021.109833

Cited by 7 publications

(1 citation statement)

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“…In comparison to other methods, in situ screening is a faster and easier way to identify a highyielding strain. It screens the strain by the morphological characteristics of the colony and the reaction of the strain with the substrate in the plate (Buffo et al 2021). A number of different methodologies have been proposed.…”

Section: Introductionmentioning

confidence: 99%

Screening of a high-yield strain of avermectin B1a by colony analysis in situ

Gou

Li²,

et al. 2022

Int Microbiol

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Avermectin, an agricultural antibiotic, is widely used as an agricultural insecticide and an important lead compound of antibiotics. It is manufactured by Streptomyces avermitilis through fermentation. Manufacturers pay special attention to screening for strains with high fermentation capacity based on morphological properties of the colony and by the result of shake ask fermentation. These traditional screening methods are time-consuming, labor-intensive and require specialized equipment. Moreover, evaluation of colony appearance is highly subjective. To improve and accelerate the screening process, we developed a rapid in situ screening method. Forty-four strains isolated naturally from the spores of industrial high-yielding strains were studied. The data show that the colony fermentation titer is highly correlated with the yield from the shake ask fermentation of avermectin, and the Pearson R is 0.990. The total titer of avermectins by shake ask fermentation is also highly correlated with the B 1a titer (Pearson R is 0.994). This result also shows that strains can be quickly screened by analyzing the colony titer.Pigment rings of the colonies that appeared after growing and maturing on the new medium plate were analyzed. The chosen colonies were directly marked and punched and then extracted with methanol. The fermentation ability can be evaluated by measuring the absorbance at 245 nm. This methodology can be applied in both natural breeding and mutation breeding conditions. By continuously breeding from 2008 to 2020, the ask titer of avermectin B 1a increased from 4582±483 µg/mL to 9197 ± 1134 µg/mL.

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Section: Introductionmentioning

confidence: 99%