Cellulolytic bacterium characterization and genome functional analysis: An attempt to lay the foundation for waste management

An, Xiaopeng; Chen, Xi; Wang, Yue; Zhao, Xinyue; Xiao, Xiaoshuang; Long, Haozhi; Li, Hanguang; Zhang, Qinghua

doi:10.1016/j.biortech.2020.124462

Cited by 18 publications

(7 citation statements)

References 31 publications

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“…and Serratia spp. [59][60][61]. They usually produce enzyme systems for lignocellulose degradation, which comprises cellulase, xylanase and cellobiase.…”

Section: Typical Microorganisms For Lignocellulose Degradationmentioning

confidence: 99%

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

et al. 2021

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Lignocellulose is a kind of renewable bioresource containing abundant polysaccharides, which can be used for biochemicals and biofuels production. However, the complex structure hinders the final efficiency of lignocellulosic biorefinery. This review comprehensively summarizes the hydrolases and typical microorganisms for lignocellulosic degradation. Moreover, the commonly used bioprocesses for lignocellulosic biorefinery are also discussed, including separated hydrolysis and fermentation, simultaneous saccharification and fermentation and consolidated bioprocessing. Among these methods, construction of microbial co-culturing systems via consolidated bioprocessing is regarded as a potential strategy to efficiently produce biochemicals and biofuels, providing theoretical direction for constructing efficient and stable biorefinery process system in the future.

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“…and Serratia spp. [59][60][61]. They usually produce enzyme systems for lignocellulose degradation, which comprises cellulase, xylanase and cellobiase.…”

Section: Typical Microorganisms For Lignocellulose Degradationmentioning

confidence: 99%

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

et al. 2021

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“…These wastes have maximum amount of cellulose which is discarded or underused, resulting pollution in the atmosphere [274,275]. Researchers have shown that characterization of cellulase producing bacteria and their genome functional analyses help to improve waste management [276]. All the wastes are now wisely used to manufacture useful goods such as enzymes, carbohydrates, biofuels, chemicals, low-cost energy fermentation streams, enhanced cattle diets, and human nutrition dietary supplements [204,277,278].…”

Section: Cellulase In Waste Managementmentioning

confidence: 99%

Cellulase: Distribution, Production, Characterization and Industrial Applications

Maravi

Kumar

2021

BJI

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Cellulase enzyme complex is comprised of three enzymes namely exo-glucanase, endo-glucanase and β-glucosidase which act synergistically to deconstruct cellulosic biomass in order to produce fermentable sugars. The enzymes are produced naturally by the living organisms such as bacteria, fungi and algae. The majority of microorganisms that live in extreme environments including hot/cold springs, rumen stomach, deep ocean trench, acidic/alkaline pH environment, have been regarded as appealing producers of cellulase. Cellulases produced by microorganisms have enormous applications in different industries such as agriculture, food and feed production, brewing, textile, laundry and biofuel production. Scientists as well as industry researchers consider cellulases as a prospective candidate for further studies due to the intricacy of the enzyme system and massive industrial potential. Scientific belief in its production and further studies challenges are receiving greater attention these days, notably in the intent of decreasing its production cost at the industrial scale. In this review, future possibilities of using cellulase for various industrial applications are also addressed.

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“…Bacterial species under the genus Serratia, belonging to the family Enterobacteriaceae, has been identified for its numerous applications in biodegradation (Majumdar et al, 2020 ; An et al, 2021 ; Dabrowska et al, 2021 ). Serratia proteamaculans , a gram-negative, non-pigmented strain under this genus, was first reported to cause a leaf spot disease and is the only identified phytopathogen under the genus Serratia (Paine and Standsfield, 1919 ; Mahlen, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Characterization of a New Serratia proteamaculans Strain That Naturally Produces Significant Amount of Extracellular Laccase

et al. 2022

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Natural biodegradation processes hold promises for the conversion of agro-industrial lignocellulosic biomaterials into biofuels and fine chemicals through lignin-degrading enzymes. The high cost and low stability of these enzymes remain a significant challenge to economic lignocellulosic biomass conversion. Wood-degrading microorganisms are a great source for novel enzyme discoveries. In this study, the decomposed wood samples were screened, and a promising γ-proteobacterial strain that naturally secreted a significant amount of laccase enzyme was isolated and identified as Serratia proteamaculans AORB19 based on its phenotypic and genotypic characteristics. The laccase activities in culture medium of strain AORB19 were confirmed both qualitatively and quantitatively. Significant cultural parameters for laccase production under submerged conditions were identified following a one-factor-at-a-time (OFAT) methodology: temperature 30°C, pH 9, yeast extract (2 g/l), Li+, Cu2+, Ca2+, and Mn2+ (0.5 mM), and acetone (5%). Under the selected conditions, a 6-fold increase (73.3 U/L) in laccase production was achieved when compared with the initial culturing conditions (12.18 U/L). Furthermore, laccase production was enhanced under alkaline and mesophilic growth conditions in the presence of metal ions and organic solvents. The results of the study suggest the promising potential of the identified strain and its enzymes in the valorization of lignocellulosic wastes. Further optimization of culturing conditions to enhance the AORB19 strain laccase secretion, identification and characterization of the purified enzyme, and heterologous expression of the specific enzyme may lead to practical industrial and environmental applications.

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Cellulolytic bacterium characterization and genome functional analysis: An attempt to lay the foundation for waste management

Cited by 18 publications

References 31 publications

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

Challenges and Future Perspectives of Promising Biotechnologies for Lignocellulosic Biorefinery

Cellulase: Distribution, Production, Characterization and Industrial Applications

Identification and Characterization of a New Serratia proteamaculans Strain That Naturally Produces Significant Amount of Extracellular Laccase

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