Cellulolytic bacteria isolation, screening and optimization of enzyme production from vermicompost of paper cup waste

Karthika, A.; Seenivasagan, R.; Kasimani, Ramesh; Babalola, Olubukola Oluranti; Muthunarayanan, Vasanthy

doi:10.1016/j.wasman.2020.06.036

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…The results on cellulase and xylanase activities show that the microbes obtained from vermicompost are able to hydrolyze plant based substrates through these enzymes. Similar results were reported for vermicompost samples by other researchers (Karthika et al 2020;Chatterjee et al, 2020). Table 1 shows the activity of different hydrolytic enzymes present in vermicompost prepared from different organic wastes.…”

Section: Analysis Of Enzyme Activitysupporting

confidence: 86%

Evaluation of Microbiological Quality of Vermicompost Prepared from Different Types of Organic Wastes using Eisenia fetida

Kapila¹,

Verma²,

Sen³

2021

ASD

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Background: Vermicomposting is the agricultural technique of conversion of organic wastes to a fertile product, which can result in better crop growth and production. However, even though earthworms are the main organisms participating in the process, the microbes associated with it also have an important role to play. These microbes degrade the waste products biochemically and are responsible of the conversion processes. Few studies are carried out on microbial diversity and related enzymes activities in the vermicompost prepared from different organic waste materials. Methods: In this paper, we isolated both bacteria and fungi from seven different types of vermicompost, using different selective media. We also studied the activity of hydrolytic enzymes that are associated with the isolated microbes.Result: It was observed that bacteria like Bacillus sp., Pseudomonas sp., Klebsiella sp., Staphylococcus aureus, Streptococcus, Micrococcus, Actinomycetes, Pigment producing Actinomycetes, Streptomyces, Azotobactor and fungi like Penicillium purpurogenum, Aspergillus sp., Alternaria alternata, Fusarium solani, Rhizopus sp., Mucor hiemalis, Myrothecium verrucaria etc. were present in our vermicompost preparations. The presence of nitrogen fixing bacteria, phosphate solubilizing microorganisms and PGPR indicated the good fertilizer value of the vermicompost samples. It was also observed that the diversity of microbes present supported significant levels of CMCase Exoglucanase, Xylanase, β-Glucosidase, Phosphatase and Urease activities.

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Section: Analysis Of Enzyme Activitysupporting

confidence: 86%

Evaluation of Microbiological Quality of Vermicompost Prepared from Different Types of Organic Wastes using Eisenia fetida

Kapila¹,

Verma²,

Sen³

2021

ASD

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“…Li et al [ 43 ] reported that most thermophiles were having optimum temperature ranging between 65 and 70 °C. Therefore, it was recognized that about 45% of cellulose degradation have occurred due to the action of bacterial consortia at 37 °C [ 44 ]. Patel et al reported the novel cellulase named Cel-5M from rumen metagenome which showed maximum activity at 40 °C [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Cloning of cellulase gene using metagenomic approach of soils collected from Wadi El Natrun, an extremophilic desert valley in Egypt

Ali

Soliman

Abdal-Aziz

et al. 2022

Journal of Genetic Engineering and Biotechnology

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Background Wadi El Natrun microorganisms have been considered as a new resource for natural products due to its extreme condition of salinity and alkalinity. Therefore, this study was devoted to generate metagemic library from soils collected from such an extreme environment in order to clone a novel cellulase for physique industrial applications. Results Total soil-DNA was successfully extracted, and then digested by different restriction enzymes. Purified fragments ranged ~ 200–6500 bp were ligated and were cloned into plasmid cloning vector (pUC19) by using Escherichia coli DH5α (E. coli) host cells. A constructed metagenomic library composed of 270 clones was screened on carboxymethylcellulose (CMC) agar plate where the active clones had been characterized by the formation of the yellowish halo zone. Thereafter, clone 1 was selected as the most active as being based on cellulase activity quantification (19 μ/ml). Plasmid related to clone 1 encoded cellSNSY gene of approximately 1.5 kb was subjected to molecular characterization; the obtained partial sequence of 861 bps encoded 287 amino acids showing 76% similarity to the endoglucanase gene of Bacillus amyloliquefaciens. The recombinant cellSNSY was expressed under lacz promoter at 1 mM of isopropyl β-d-1-thiogalactopyranoside (IPTG), giving 21 μ/ml cellulase after ~ 27 h. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and an activity staining of the recombinant cellSNSY which revealed an active band with a molecular mass ~ 59 kDa appeared in the induced sample. The maximum enzyme activity of crude cellSNSY was observed at 45 °C and for a pH of 8.5. Interestingly, the enzyme activity was slightly inhibited by ethylenediamine tetraacetic acid (EDTA) and methanol. It showed high resistance to the tested heavy metals and the surfactant which ordered Zn> (SDS,Fe)>Mn>Cu. Conclusions This study established an easy and a skillful way to clone/express a new found cellulase gene(s) under lacZ promoter. The isolated recombinant cellSNSY showed 76% similarity to endoglucanase gene, and the enzyme showed tolerance to the mostly tested agents including heavy metals, surfactant, solvents, and EDTA. Additionally, the studied recombinant showed a high stability up to 55 °C and for alkaline pH 8.5. These features make it an ample and viable for many applications.

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“…However, most Acinetobacter bacteria are important soil organisms and contribute to aromatic compound mineralization. Some studies also found that the genus Acinetobacter bacteria could hydrolyze cellulose efficiently (Pourramezan et al, 2012;Karthika et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of cellulose-degrading endophytic bacteria from Tomoceridae (springtails)

Wei¹,

Miao²,

Tian³

et al. 2022

Afr. J. Microbiol. Res.

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Springtails are considered as an important candidate bioindicator to assess soil quality spiked with trace metals, but little is known of their endophytic bacteria. In this study, a kind of Tomoceridae springtail was used, and a total of 45 effective sequences were obtained through the process of endophytic bacteria isolation, culture, polymerase chain reaction (PCR) amplification and sequencing. After NCBI-BLAST, the results showed that there were 20 bacterial colonies belonging to the genus Staphylococcus, 12 belonging to the genus Bacillus, 7 belonging to the genus Paenibacillus, 1 belonging to the genus Exiguobacterium and 5 belonging to Acinetobacter lwoffii. Furthermore, five bacterial strains from these five genera (named TomoRZH14, TomoRZH26, TomoRZH30, TomoRZH37, TomoRZH40) were selected for cellulose degradation analysis. The results showed that TomoRZH26 (Bacillus sp.) seemed to have a stronger cellulose degradation ability than those of the other four strains, while the three main components cellulase endo-β-glucanase, exo-β-glucanase and βglucosidase in TomoRZH26 showed significantly higher enzymatic activity than in the other strains. Viscosity analysis also showed that the TomoRZH26 bacterium degraded relatively quickly in cellulase fermentation medium. In general, in this study, we preliminarily revealed several endophytic bacteria of Tomoceridae springtails and found that they had potentially strong cellulose degradation activity, which may be one of the important reasons behind springtail adaptation to this kind of soil ecological environment.

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Cellulolytic bacteria isolation, screening and optimization of enzyme production from vermicompost of paper cup waste

Cited by 29 publications

References 29 publications

Evaluation of Microbiological Quality of Vermicompost Prepared from Different Types of Organic Wastes using Eisenia fetida

Evaluation of Microbiological Quality of Vermicompost Prepared from Different Types of Organic Wastes using Eisenia fetida

Cloning of cellulase gene using metagenomic approach of soils collected from Wadi El Natrun, an extremophilic desert valley in Egypt

Isolation and identification of cellulose-degrading endophytic bacteria from Tomoceridae (springtails)

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