Cellulase production by T. reesei

Réczey, Kati; Szengyel, Zsolt; Eklund, Robert; Zacchi, Guido

doi:10.1016/0960-8524(96)00038-7

Cited by 112 publications

(52 citation statements)

References 21 publications

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“…IFPU/L-h at 9 days and with a casing loading of 25g/L was 0.77 IFPU/ml at 6 days and 5.1 IFPU/L-h at 5 days. The cellulase activity for all the loading levels was within the values obtained in other studies using waste paper and biomass as substrate with T. reesei RUT-30 culture (Silvia et al, 1995;Reczey et al, 1996;Hayward et al, 1999;Shin et al, 2000).…”

Section: Cellulase Production In the Bubble-column Reactorsupporting

confidence: 62%

“…As a result, extensive studies with promising cellulase enzyme yields have been obtained in the fermentation of T. reesei RUT-C30 with different solid lignocellulosic materials and cellulosic waste products such as wheat straw, bagasse, aspen wood, oak wood chips, willow, yellow poplar, waste paper sludge, waste newsprint, office paper, municipal solid waste, and banana waste (Reczey et al, 1996;Shin et al, 2000;Szengyel et al, 2000;Reddy et al, 2003). Soluble carbon sources such as lactose, xylose, and cheese-whey permeate have also been used for cellulase production but with only some strains of T. reesei (Mohagheghi et al, 1988;Cameron, 1996;Reczey et al, 1996).…”

Section: Cellulase Productionmentioning

confidence: 99%

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Production of Value-Added Products from Meat Processing Cellulosic Waste

Cumba¹,

Bellmer²

2005

2005 Tampa, FL July 17-20, 2005

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Section: Cellulase Production In the Bubble-column Reactorsupporting

confidence: 62%

Section: Cellulase Productionmentioning

confidence: 99%

Production of Value-Added Products from Meat Processing Cellulosic Waste

Cumba¹,

Bellmer²

2005

2005 Tampa, FL July 17-20, 2005

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“…Cellulase, which is expressed as filter paper activity (FPA), was measured according to Reczey et al (1996), with one unit defined as formation of 1 µmol glucose per hour. LiP activity was measured as described by Roy and Archibald (1993), with 1 U defined as 1/µmol of veratryl alcohol oxidized to vertraldehyde per min.…”

Section: Methodsmentioning

confidence: 99%

Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium

Liu

et al. 2016

BioResources

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The potential of Phanerochaete chrysosporium in ethanol fermentation was evaluated. During the initial submerged cultivation, 1.76 g/L ethanol was obtained using glucose as substrate. After mutation, the ethanol concentration of an alcohol dehydrogenase (ADH) mutant reached 5.02 g/L. Both base transition and nine-base frame shift mutation occurred in the ADH gene of the mutant, changing the secondary and tertiary structures of ADH, as well as increasing the ADH activity during cultivation. Moreover, P. chrysosporium converted corn stalk to ethanol by a one-step process. After statistical optimizations, 0.26 g/g•substrate of ethanol yield was obtained on day 10. During the fermentation, the maximum lignin peroxidase, Mn-dependent peroxidase, and cellulase activities were 29.0 U/L, 256.5 U/L, and 40 U/mL, respectively, thus explaining why the fungus directly ferments corn stalk to ethanol. This study is the first report of the conversion of corn stalk without pretreatment to ethanol using a white-rot fungus.

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“…Economical production of cellulases is key for feasible bioethanol production from lignocellulosic biomass using cellulase-based processes. To date the production of cellulase has been widely studied in submerged culture processes (liquid state) but the relatively high cost of enzyme production has hindered the industrial applications through various expensive media are used to produce such enzymes (Reczey et al, 1996). The aim of this study was to determine the fermentation conditions by applying the statistical technique utilizing Palm Oil Mill Effluent (POME) as new substrate for the production of cellulase enzyme.…”

Section: Introductionmentioning

confidence: 99%

Statistical Optimization of Fermentation Conditions for Cellulase Production from Palm Oil Mill Effluent

Daoud¹

2010

American Journal of Environmental Sciences

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Problem statement: Palm oil mill effluent discharged by the oil palm industries is considered as the mixed of high polluted effluent which is abundant (about 20 million tonnes year −1 ) and its effect contributes to the serious environmental problems through the pollution of water bodies. Approach: The aim of this study was to identify the potential of low cost substrate such as Palm Oil Mill Effluent (POME) for the production of cellulase enzyme by liquid state bioconversion. The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation.Results: Statistical analysis showed that the optimum conditions were: Temperature of 30°C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU mL −1 . Analysis Of Variance (ANOVA) of the design showed a high coefficient of determination (R 2 ) value of 0.999, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data. Conclusion/Recommendations: This study indicates a better solution for waste management through the utilization of POME for cellulase production that could be used in the industrial applications such as bioethanol production.

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Cellulase production by T. reesei

Cited by 112 publications

References 21 publications

Production of Value-Added Products from Meat Processing Cellulosic Waste

Production of Value-Added Products from Meat Processing Cellulosic Waste

Conversion of Corn Stalk to Ethanol by One-Step Process using an Alcohol Dehydrogenase Mutant of Phanerochaete chrysosporium

Statistical Optimization of Fermentation Conditions for Cellulase Production from Palm Oil Mill Effluent

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