Cellulase Production by Streptomyces viridobrunneus SCPE-09 Using Lignocellulosic Biomass as Inducer Substrate

Abstract

An actinomycete strain, isolated from a soil sample under a sugar cane plantation in Brazil and identified as Streptomyces viridobrunneus SCPE-09, was selected as a promising cellulolytic strain, and tested for its ability to produce cellulases from agro-industrial residues. Sugar cane bagasse or wheat bran was tested as carbon source, and corn steep liquor tested as nitrogen source. Different concentrations of carbon and nitrogen were tested using factorial design to identify optimal cellulose production. The results showed that media containing wheat bran 2.0% (w/v) and corn steep liquid 0.19% (w/v) lead to the highest production, 2.0 U mL(-1) of CMCase, obtained on the fifth day of fermentation. The pH and temperature profile showed optimal activity at pH 4.9 and 50°C. As for thermostability, endoglucanases were most tolerant at 50°C, retaining more than 80% of maximal activity even after 2 h of incubation. Zymogram analyses using supernatant from growth under optimized conditions revealed the presence of two CMCase bands with apparent molecular masses of 37 and 119 kDa. The combination of pH tolerance and CMCase production from agro-industrial residues by S. viridobrunneus SCPE-09 offers promise for future bioethanol biotechnologies.

Production and Characterization of Partially Purified Thermostable Endoxylanase and Endoglucanase from Novel Actinomadura geliboluensis and the Biotechnological Applications in the Saccharification of Lignocellulosic Biomass

“…11b). The thermostability of endoglucanase that was produced by A. geliboluensis was better than that of some actinomycetes such as Streptomyces malaysiensis (Nascimento et al 2009), Streptomyces viridobrunnes SCPE-09 (Da Vinha et al 2011), and Streptomyces drozdowiczii (De Lima et al 2005). However, endoglucanase that was produced by Thermomonospora sp.…”

Production of cellulose-degrading enzyme on sisal and other agro-industrial residues using a new Brazilian actinobacteria strain Streptomyces sp. SLBA-08

“…Cells were cultivated in a mineral medium (Da Vinha et al, 2011) Fermentations were performed in 250-mL Erlenmeyer flasks containing 50 mL of the culture medium (initial pH 6.8). Each medium was inoculated with 50 µL of spore suspension (1.61 x 10 8 spores mL ), for 5 days.…”

“…Crude enzyme was able to retain 70% residual activity at 50 °C for 2 h, the half-life being 6 h at this temperature. Half-lives of 24 h at 40 °C or 8 h at 50 °C have been cited in the literature for some Streptomyces strains (Grigorevski-Lima et al, 2005;Nascimento et al, 2009;Da Vinha et al, 2011). The pH activity profile of the crude extract obtained under the best growth conditions (Fig.…”

“…The Streptomyces are the most important genus in this group, able to produce and excrete a large variety of enzymes, including those involved in the degradation of cellulose, hemicellulose and lignin. Since the microbiology of Brazilian tropical soils is largely unknown, these soils can represent an excellent source for the search for new species and enzymes, especially cellulases (Grigorevski-Lima Brazilian Journal of Chemical Engineering Nascimento et al, 2009;Da Vinha et al, 2011). These cellulose degrading enzymes are related to industrial processes, like food, brewery and wine, agriculture, textile, detergent, animal feed, pulp and paper industries, as well as research and development technologies (Bhat, 2000;Jang and Chen, 2003;.…”

“…Studies dealing with cellulase (CMCase) production by actinobacteria using low-cost agro-industrial residues are scarce in literature, however our group has been isolating cellulolytic strains from Brazilian tropical soils for some time, obtaining promising results (Grigorevski-Lima et al, 2005;Nascimento et al, 2009;Da Vinha et al, 2011;Nascimento and Coelho, 2011).…”