Cellulase Hyper-Producing Fungus Penicillium janthinellum NCIM 1366 Elaborates a Wider Array of Proteins Involved in Transport and Secretion, Potentially Enabling a Diverse Substrate Range

Christopher, Meera; Sreeja-Raju, AthiraRaj; Kooloth-Valappil, Prajeesh; Gokhale, D. V.; Sukumaran, Rajeev K.

doi:10.1007/s12155-022-10407-3

Cited by 4 publications

(2 citation statements)

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“…PJ-1366 encodes 11,828 proteins, of which 1007 have signal peptides 7 . Of the extracellular proteins, 216 proteins were described as carbohydrate-active enzymes (CAZymes), of which 165 were Glycoside Hydrolases (GH), that include the cellulases and hemicellulases 8 . Among them, 40 sequences were identified as belonging to putative cellulase families GH2, GH3, GH30, GH5, GH6, GH7, GH12, GH45 and GH131.…”

Section: Resultsmentioning

confidence: 99%

Early cellular events and potential regulators of cellulase induction in Penicillium janthinellum NCIM 1366

Christopher

Sreeja-Raju

Abraham

et al. 2023

Sci Rep

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Cellulase production by fungi is tightly regulated in response to environmental cues, and understanding this mechanism is a key pre-requisite in the efforts to improve cellulase secretion. Based on UniProt descriptions of secreted Carbohydrate Active enZymes (CAZymes), 13 proteins of the cellulase hyper-producer Penicillium janthinellum NCIM 1366 (PJ-1366) were annotated as cellulases- 4 cellobiohydrolases (CBH), 7 endoglucanases (EG) and 2 beta glucosidases (BGL). Cellulase, xylanase, BGL and peroxidase activities were higher for cultures grown on a combination of cellulose and wheat bran, while EG was stimulated by disaccharides. Docking studies indicated that the most abundant BGL- Bgl2- has different binding sites for the substrate cellobiose and the product glucose, which helps to alleviate feedback inhibition, probably accounting for the low level of glucose tolerance exhibited. Out of the 758 transcription factors (TFs) differentially expressed on cellulose induction, 13 TFs were identified whose binding site frequencies on the promoter regions of the cellulases positively correlated with their abundance in the secretome. Further, correlation analysis of the transcriptional response of these regulators and TF-binding sites on their promoters indicated that cellulase expression is possibly preceded by up-regulation of 12 TFs and down-regulation of 16 TFs, which cumulatively regulate transcription, translation, nutrient metabolism and stress response.

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Section: Resultsmentioning

confidence: 99%

Early cellular events and potential regulators of cellulase induction in Penicillium janthinellum NCIM 1366

Christopher

Sreeja-Raju

Abraham

et al. 2023

Sci Rep

Self Cite

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“…The authors proposed that with proper supply chain management, this fermentable carbon can be destined as a co-feed in any existing distillery, or even a separate decentralized system could be envisaged. Christopher et al [14] worked on the cellulase hyper-producing fungus Penicillium janthinellum NCIM 1366 and presented a wider array of proteins involved in transport and secretion, potentially enabling a diverse substrate range.…”

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confidence: 99%

Biotechnology for Resource Efficiency, Energy, Environment, Chemicals, and Health

et al. 2023

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There has been an increasing role and significance of industrial bioprocessing and sustainable developments in different walks of life-be it human life or animal life, plant life, or overall on the environment, which has attained global relevance related to sustainable development goals. Biotechnological innovations are generally sustainability-conscious and have a pivotal role in the current scenario .

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Novel cyclic shifting of temperature strategy for simultaneous saccharification and fermentation for lignocellulosic bioethanol production

Panda,

Maiti

2024

Bioresource Technology

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Cellulase Hyper-Producing Fungus Penicillium janthinellum NCIM 1366 Elaborates a Wider Array of Proteins Involved in Transport and Secretion, Potentially Enabling a Diverse Substrate Range

Cited by 4 publications

References 50 publications

Early cellular events and potential regulators of cellulase induction in Penicillium janthinellum NCIM 1366

Early cellular events and potential regulators of cellulase induction in Penicillium janthinellum NCIM 1366

Biotechnology for Resource Efficiency, Energy, Environment, Chemicals, and Health

Novel cyclic shifting of temperature strategy for simultaneous saccharification and fermentation for lignocellulosic bioethanol production

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