Cellulase enzyme activity of the bacteria isolated from mangrove ecosystem in Aceh Besar and Banda Aceh

Abstract

Cellulolytic bacteria is one of the beneficial bacteria that can found in mangrove ecosystem. The purposes of study were to analyse the cellulolytic index, and to analyse the cellulase activity of bacteria isolated from soil mangrove. Qualitatively, assessment of cellulase activity were carried out at the Microbiology laboratory of Fish Quarantine Station, Quality Control and Safety of Fishery Products (SKIPM) Aceh, while quantitatively was observed in microbiology laboratory, Biology Department, IPB. Assessment of qualitative cellulase activity is performed by growing the selected pure isolate on 1% CMC medium then spilled 1% congo red to test its cellulolytic potential. Cellulolytic potential was determined by clear zone performed around the colony after congo red flooded. The quantitative cellulase enzyme activity test carried out by DNS method tested on one selective isolate. There were 21 from 39 isolates showed a clear zone isolated from mangrove soil. The cellulolytic index (CI) obtained ranged from 0.07 to 0.80 classified as low cellulolytic index criteria. The cellulolytic index was higher in bacteria isolated from mangrove rehabilitated than mangrove unrehabilitated. The highest cellulase activity and specific cellulase activity of BTMD32 was at 48 hours with the value were 0.0012 U/ml, 0.077 U/mg, respectively. The result concluded that the bacteria cellulolytic isolated from mangrove soil had low cellulolytic index, low cellulase activity, and low specific cellulase activity.

Production and activity optimization of cellulase and xylanase from the compost isolated Paracoccus kondratievae strain GB in Egypt

“…Our data agree with those of (Fatokun et al 37) who reported that the best agitation rate for xylanase was 150 rpm and for cellulase was between 100 and 150 rpm. Nutrient supplies have been reported to be key elements in the synthesis of cellulase and xylanase, and carbon source is thought to be an essential nutrient affecting enzyme production (45). We tested the impact of different carbon sources, such as glucose, sucrose, mannose, arabinose, lactose, CMC, xylan, glucose + CMC, glucose + xylan and filter paper on enzyme production.…”

Selection of Pectinolytic Yeast from Liberica Green Coffee Beans (Coffea liberica)

“…1). The pectinolytic index produced by isolates ranged from 0 -0.22 and was classified as low according to Dewiyanti et al [18]. The isolate that had the highest pectinolytic index was Y5, but there were no significant differences in value between isolates.…”

“…According to Haile & Kang, incubation time is one of the factors that influences pectinase enzyme production [12]. It is said that a higher pectinolytic index can be used as an indication of higher enzyme activity [18]. However, it requires further quantitative assay to exactly know the enzyme activity of each isolate.…”

Production of Sugar from Cassava Peel using Different Chemical Pre-treatment

“…Cellulase may be produced by several microorganisms: actinomycetes, bacteria, and fungi. Each cellulolytic microorganism has a difference in the cellulase activity produced [75] A study by [42] found that Aspergillus niger produced the highest glucose concentration with the highest enzymatic activity (0.13 U/ml), followed by a monoculture of Trichoderma reesei (0.11 U/ml). Besides, [42] reported that Penicillium citrinum and Bacillus subtilis have the activity of 1.72 IU/ml and 2.8 IU/gds, respectively.…”