Cellular uptake of pH/reduction responsive phosphorylcholine micelles

Cai, Yuanyuan; Li, Shuai; Cai, Mengtan; Chen, Yuanwei; Luο, Xingguang

doi:10.1039/c7nj02484c

Cited by 9 publications

(15 citation statements)

References 45 publications

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“…1 H NMR spectra of the precursors and targets were recorded on a spectrometer (Unity Inova 400) operating at 400 MHz using CDCl 3 or CD 3 OD as a solvent with tetramethylsilane (TMS) as the internal standard …”

Section: Methodsmentioning

confidence: 99%

“…Attenuated total reflection Fourier transform infrared (ATRFTIR) spectra were taken on a FTIR spectrometer (Nicolet Magna 560) …”

Section: Methodsmentioning

confidence: 99%

“…Cai et al developed pH/reduction responsive copolymer poly(ε-caprolactone)-ss- b -poly((N,N-diethylaminoethyl methacrylate)-r-poly(2-methacryloyloxyethyl phosphorylcholine) (PCL-ss-PDEAPMPC). The cytotoxicity of PCL-ss-PDEAPMPC micelles was low; on the contrary, the drug-loaded micelles strongly inhibited the growth of tumor cells because of fast drug release under the simultaneously reductive and acidic microenvironment of tumors …”

Section: Introductionmentioning

confidence: 98%

“…The cytotoxicity of PCL-ss-PDEAPMPC micelles was low; on the contrary, the drug-loaded micelles strongly inhibited the growth of tumor cells because of fast drug release under the simultaneously reductive and acidic microenvironment of tumors. 35 Although a number of pH-sensitive amphiphilic polymers containing ionizable groups has been reported, there are few study on complete comparison of in vitro and in vivo antitumor efficiency of pH-sensitive micelles with tertiary amines as sensitive groups with insensitive micelles, especially about phosphorylcholine micelles. With these considerations in mind, we designed and synthesized a novel pH-sensitive amphiphilic polymer poly{α- [4-(diethylamino)methyl-1,2,3triazol]-caprolactone-co-caprolactone}-b-poly(2-methacryloyloxyethyl phosphorylcholine) (PDCL-PMPC), and poly-(caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) (PCL-PMPC) without pH-sensitive groups was synthesized as a control.…”

Section: Introductionmentioning

confidence: 99%

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Tracing Difference: In Vitro and in Vivo Antitumor Property Comparison of pH-Sensitive Biomimetic Phosphorylcholine Micelles with Insensitive Micelles

Wang

Lei

Chen

et al. 2019

ACS Biomater. Sci. Eng.

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Although smart polymer micelles able to respond to the tumor acid microenvironment are potential anticancer drug carriers, fruitful clinical application of these carriers is inadequate. For the purpose of finding the origin of the unsatisfactory therapeutic efficacy of pH-sensitive drug carriers with tertiary amine groups, we newly designed and synthesized poly{α-[4-(diethylamino)methyl-1,2,3-triazol]-caprolactone-co-caprolactone}-b-poly(2-methacryloyloxyethyl phosphorylcholine) (PDCL-PMPC), a biomimetic phosphorylcholine polymer with pH-ensitive groups. PDCL-PMPC self-assembles into small and uniform micelles as its counterpart poly(caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) (PCL-PMPC) without pH-sensitive groups. The in vitro and in vivo properties of PDCL-PMPC and PCL-PMPC micelles are investigated in detail. PDCL-PMPC micelles display obvious pH sensitivity by micelle change and fast drug release at pH 5, but the insensitive micelles do not. The internalization of PDCL-PMPC micelles by tumor cells is stronger than that of PCL-PMPC micelles. However, in comparison with the insensitive micelles, the pH-sensitive micelles present much shorter blood circulation time in pharmacokinetics and demonstrate worse accumulation in the tumor site in vivo study. As a result, DOX loaded PCL-PMPC micelles demonstrate much better antitumor efficiency than pH-sensitive micelles. Furthermore, DOX loaded PCL-PMPC micelles show similar therapeutic efficacy to DOX·HCl but with considerably lower side effects.

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Section: Methodsmentioning

confidence: 99%

“…Attenuated total reflection Fourier transform infrared (ATRFTIR) spectra were taken on a FTIR spectrometer (Nicolet Magna 560) …”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Tracing Difference: In Vitro and in Vivo Antitumor Property Comparison of pH-Sensitive Biomimetic Phosphorylcholine Micelles with Insensitive Micelles

Wang

Lei

Chen

et al. 2019

ACS Biomater. Sci. Eng.

Self Cite

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show abstract

“…In the second BTAMDL method, the GBDT input also includes additional features. Furthermore, consensus strategy, 35,36 and feature selection from importance ranking, [37][38][39][40] are implemented in the present work. The proposed methods are validated on various datasets, and the results of our numerical experiments confirm that the present models indeed achieve better performance on tasks with small data and outperform other state-of-the-art ML methods.…”

Section: Introductionmentioning

confidence: 99%