Cellular Uptake of Magnetic Nanoparticles Quantified by Magnetic Particle Spectroscopy

Loewa, Norbert; Wiekhorst, Frank; Gemeinhardt, Ines; Ebert, Monika; Schnorr, Jörg; Wagner, Susanne; Taupitz, Matthias; Trahms, Lutz

doi:10.1109/tmag.2012.2218223

Cited by 18 publications

(19 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Diamagnetic biological tissue and paramagnetic blood iron do not contribute to the MPS signal. 19 For measurement, a PCR tube typically containing 30-50 L sample volume was placed in the pick-up Copyright: American Scientific Publishers coil of the MPS system. During measurement, the sample was exposed to a sinusoidal oscillating magnetic field (variable amplitude B excit up to 25 mT, fixed frequency f excit of 25 kHz) and the non-linear magnetization response was detected (sensitivity: 5 · 10 12 Am 2 ).…”

Section: Methodsmentioning

confidence: 99%

“…The amplitude of the third harmonic in the MPS signal, A 3 , allows for specific determination of SPIO iron amount without being affected by non-particular body iron. 19 Changes in the nonlinear response (as parameterized by the harmonic ratio A 5 /A 3 provide information on the magnetic characteristics of SPIO. 20 The present study evaluates the feasibility and accuracy of MPS for VSOP quantification and for analysis of labeling efficacy in living THP-1 cells.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Magnetic Particle Spectroscopy Reveals Dynamic Changes in the Magnetic Behavior of Very Small Superparamagnetic Iron Oxide Nanoparticles During Cellular Uptake and Enables Determination of Cell-Labeling Efficacy

Poller¹,

Löwa²,

Wiekhorst³

et al. 2016

j biomed nanotechnol

Self Cite

View full text Add to dashboard Cite

In vivo tracking of nanoparticle-labeled cells by magnetic resonance imaging (MRI) crucially depends on accurate determination of cell-labeling efficacy prior to transplantation. Here, we analyzed the feasibility and accuracy of magnetic particle spectroscopy (MPS) for estimation of cell-labeling efficacy in living THP-1 cells incubated with very small superparamagnetic iron oxide nanoparticles (VSOP). Cell viability and proliferation capacity were not affected by the MPS measurement procedure. In VSOP samples without cell contact, MPS enabled highly accurate quantification. In contrast, MPS constantly overestimated the amount of cell associated and internalized VSOP. Analyses of the MPS spectrum shape expressed as harmonic ratio A₅/A₃ revealed distinct changes in the magnetic behavior of VSOP in response to cellular uptake. These changes were proportional to the deviation between MPS and actual iron amount, therefore allowing for adjusted iron quantification. Transmission electron microscopy provided visual evidence that changes in the magnetic properties correlated with cell surface interaction of VSOP as well as with alterations of particle structure and arrangement during the phagocytic process. Altogether, A₅/A₃-adjusted MPS enables highly accurate, cell-preserving VSOP quantification and furthermore provides information on the magnetic characteristics of internalized VSOP.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Magnetic Particle Spectroscopy Reveals Dynamic Changes in the Magnetic Behavior of Very Small Superparamagnetic Iron Oxide Nanoparticles During Cellular Uptake and Enables Determination of Cell-Labeling Efficacy

Poller¹,

Löwa²,

Wiekhorst³

et al. 2016

j biomed nanotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…In another technique called magnetic particle spectroscopy (MPS) or magnetization response spectroscopy, frequency and large amplitude is applied to IONPs and the non-linear magnetization response of IONPs in the presence of an oscillating magnetic field is monitored [ 167 ]. The MPS was recently utilized to quantify IONPs in Hela and Jurkat cells with the detection limits of 5 ng for Resovist, 50 ng for Ferraheme, and 100 ng for carboxydextran coated IONPs [ 168 ]. MPS measurements utilized small sample volumes (5 µL), allowed rapid measurements, and a high throughput screening (200 samples per hour).…”

Section: Quantification Of Ionpsmentioning

confidence: 99%

In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

Patil

Adireddy

Jaiswal

et al. 2015

IJMS

169

117

View full text Add to dashboard Cite

Increasing biomedical applications of iron oxide nanoparticles (IONPs) in academic and commercial settings have alarmed the scientific community about the safety and assessment of toxicity profiles of IONPs. The great amount of diversity found in the cytotoxic measurements of IONPs points toward the necessity of careful characterization and quantification of IONPs. The present document discusses the major developments related to in vitro and in vivo toxicity assessment of IONPs and its relationship with the physicochemical parameters of IONPs. Major discussion is included on the current spectrophotometric and imaging based techniques used for quantifying, and studying the clearance and biodistribution of IONPs. Several invasive and non-invasive quantification techniques along with the pitfalls are discussed in detail. Finally, critical guidelines are provided to optimize the design of IONPs to minimize the toxicity.

show abstract

“…For example, distinguishing free MNPs from those that are bound to cells. Strategies for probing the local environmental status of MNPs (e.g., bound vs. unbound) include detection of even, odd, and intermodulation frequencies [28-32], the use of ratios between harmonics[21, 33], rotational drift non-linearity [34] and magnetic particle spectroscopy (MPS) [35, 36]. MPS is a technique that uses a large applied field in order to measure a number of harmonic frequencies to determine a spectroscopic difference in the MNP response when MNPs are in a bound state.…”

Section: Introductionmentioning

confidence: 99%

A Feasibility Study of Nonlinear Spectroscopic Measurement of Magnetic Nanoparticles Targeted to Cancer Cells

Ficko

Ndong²,

Giacometti³

et al. 2017

IEEE Trans. Biomed. Eng.

View full text Add to dashboard Cite

Objective Magnetic nanoparticles (MNPs) are an emerging platform for targeted diagnostics in cancer. An important component needed for translation of MNPs is the detection and quantification of targeted MNPs bound to tumor cells. Method This study explores the feasibility of a multi-frequency nonlinear magnetic spectroscopic method that uses excitation and pick-up coils and is capable of discriminating between quantities of bound and unbound MNPs in 0.5 ml samples of KB and Igrov human cancer cell lines. The method is tested over a range of five concentrations of MNPs from 0 to 80 μg /ml and five concentrations of cells from 50 to 400 thousand count per ml. Results A linear model applied to the magnetic spectroscopy data was able to simultaneously measure bound and unbound MNPs with agreement between the model-fit and lab assay measurements (p<0.001). The detectable iron of the presented method to bound and unbound MNPs was < 2 μg in a 0.5 ml sample. The linear model parameters used to determine the quantities of bound and unbound nanoparticles in KB cells were also used to measure the bound and unbound MNP in the Igrov cell line and vice versa. Conclusion Nonlinear spectroscopic measurement of magnetic nanoparticles may be a useful method for studying targeted MNPs in oncology. Significance Determining the quantity of bound and unbound MNP in an unknown sample using a linear model represents an exciting opportunity to translate multi-frequency nonlinear spectroscopy methods to in vivo applications where MNPs could be targeted to cancer cells.

show abstract

Cellular Uptake of Magnetic Nanoparticles Quantified by Magnetic Particle Spectroscopy

Cited by 18 publications

References 31 publications

Magnetic Particle Spectroscopy Reveals Dynamic Changes in the Magnetic Behavior of Very Small Superparamagnetic Iron Oxide Nanoparticles During Cellular Uptake and Enables Determination of Cell-Labeling Efficacy

Magnetic Particle Spectroscopy Reveals Dynamic Changes in the Magnetic Behavior of Very Small Superparamagnetic Iron Oxide Nanoparticles During Cellular Uptake and Enables Determination of Cell-Labeling Efficacy

In Vitro/In Vivo Toxicity Evaluation and Quantification of Iron Oxide Nanoparticles

A Feasibility Study of Nonlinear Spectroscopic Measurement of Magnetic Nanoparticles Targeted to Cancer Cells

Contact Info

Product

Resources

About