Cellular Uptake of Gold Nanoparticles into Normal and Cancer Cells

Trono, Jade; Mizuno, Kazue; Yusa, Noritaka; Matsukawa, Tetsuya; Uesaka, Mitsuru

doi:10.1007/978-3-642-03887-7_56

Cited by 10 publications

(7 citation statements)

References 9 publications

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“…Our observations agree with those reported by other authors regarding the cell lines of mouse calvarial osteoblasts (MC3T3 E1),36 pancreatic cancer (PK-1, PK-45, and Panc-1),37 and liver cancer (Hep-G2),38 located on the plasma membrane, obtained by TEM, SEM, and CLSM.…”

Section: Resultssupporting

confidence: 93%

Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

Lara-Cruz

Jiménez-Salazar

Ramón-Gallegos

et al. 2016

IJN

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Gold nanoparticles (AuNPs) have been proposed for use in the treatment of different types of cancer, including breast cancer. At present, neither the mechanisms of AuNP interaction with the plasma membrane surface and their delivery and intracellular distribution in cancer cells nor their effect on the plasma membrane so as to allow cell incorporation of larger amounts of AuNPs is known. The objective of this work was to study the interaction of bare 20 nm diameter AuNPs with the plasma membrane of human MCF-7 breast cancer cells, as well as their uptake, intracellular distribution, and induction of changes on the cell surface roughness. The dynamics of intracellular incorporation and the distribution of AuNPs were observed by confocal laser scanning microscopy. Changes in roughness were monitored in synchronized MCF-7 cells by atomic force microscopy high-resolution imaging at 6 hour intervals for 24 hours during a single cell cycle. The results show that bare AuNPs are capable of emitting fluorescence at 626 nm, without the need for a fluorescent biomarker, which allows monitoring their uptake and intracellular distribution until they reach the nucleus. These results are correlated with changes in cell roughness, which significantly increases at 12 hours of incubation with AuNPs, when compared with control cells. The obtained data provide bases to understand molecular processes of the use of AuNPs in the treatment of different diseases, mainly breast cancer.

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Section: Resultssupporting

confidence: 93%

Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

Lara-Cruz

Jiménez-Salazar

Ramón-Gallegos

et al. 2016

IJN

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“…It was seen from Figure 1 , that at concentrations greater > 1 mM, the level of gold within the non-cancerous cells was proportional to the external concentration, however in the tumour cells intracellular levels did not increase above this concentration. These results are in agreement with that reported by Trono et al [ 22 ]. They observed that uptake and incorporation of AuNPs depend on various factors such as cell type (non-cancerous or cancerous), the concentration and size of the nanoparticles and the incubation period with the nanoparticles.…”

Section: Discussionsupporting

confidence: 94%

Differential Effects of Gold Nanoparticles and Ionizing Radiation on Cell Motility between Primary Human Colonic and Melanocytic Cells and Their Cancerous Counterparts

Shahhoseini

Nakayama

Piva

et al. 2021

IJMS

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This study examined the effects of gold nanoparticles (AuNPs) and/or ionizing radiation (IR) on the viability and motility of human primary colon epithelial (CCD841) and colorectal adenocarcinoma (SW48) cells as well as human primary epidermal melanocytes (HEM) and melanoma (MM418-C1) cells. AuNPs up to 4 mM had no effect on the viability of these cell lines. The viability of the cancer cells was ~60% following exposure to 5 Gy. Exposure to 5 Gy X-rays or 1 mM AuNPs showed the migration of the cancer cells ~85% that of untreated controls, while co-treatment with AuNPs and IR decreased migration to ~60%. In the non-cancerous cell lines gap closure was enhanced by ~15% following 1 mM AuNPs or 5 Gy treatment, while for co-treatment it was ~22% greater than that for the untreated controls. AuNPs had no effect on cell re-adhesion, while IR enhanced only the re-adhesion of the cancer cell lines but not their non-cancerous counterparts. The addition of AuNPs did not enhance cell adherence. This different reaction to AuNPs and IR in the cancer and normal cells can be attributed to radiation-induced adhesiveness and metabolic differences between tumour cells and their non-cancerous counterparts.

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“…On the other hand, gold nanoparticles (AuNPs) has been investigated as an anti‐cancer reagent in various fields for decades, such as drug delivery agent, radio sensitizer, and photothermal reagent . These applications imply that the uptake of AuNPs contributes an important part in the investigation.…”

Section: Introductionmentioning

confidence: 99%

Cold Plasma Accelerates the Uptake of Gold Nanoparticles Into Glioblastoma Cells

Cheng

Rajjoub

Sherman

et al. 2015

Plasma Processes & Polymers

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The gold nanoparticles and cold plasma have been widely investigated separately in cancer therapy. In this paper, the two agents were combined in order to achieve a more profound understanding of their interactions in glioblastoma (U87) and normal human astrocytes (E6/E7). Gold nanoparticles were endocytosed at an accelerated rate in the U87 cell membrane due to the plasma treatment. This caused many divots across the cell membrane which made it more porous. On the contrary, in E6/E7 cells, there was no significant difference for penetration of gold nanoparticles or the cell membrane morphology. As proven in previous experiments the addition of the nanoparticles decrease cell viability, allowing the cold plasma to be more efficient. Present work demonstrated the synergistic effect of the two agents, which can be correlated in order to maximize the efficiency.

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Cellular Uptake of Gold Nanoparticles into Normal and Cancer Cells

Cited by 10 publications

References 9 publications

Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

Increasing roughness of the human breast cancer cell membrane through incorporation of gold nanoparticles

Differential Effects of Gold Nanoparticles and Ionizing Radiation on Cell Motility between Primary Human Colonic and Melanocytic Cells and Their Cancerous Counterparts

Cold Plasma Accelerates the Uptake of Gold Nanoparticles Into Glioblastoma Cells

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