Cellular Uptake Mechanism of Cationic Branched Polypeptides with Poly[l-Lys] Backbone

Abstract

Cationic macromolecular carriers can be effective carriers for small molecular compounds, drugs, epitopes or nucleic acids. Polylysine based polymeric branched polypeptides have been systematically studied on the level of cells and organisms as well. In the present study we report on our findings on the cellular uptake characteristics of nine structurally related polylysine based

Cationic dynamic covalent polymers for gene transfection

“…102 The high loading of histidine into cationic polymers effectively promote efficient endosomal escape but can also sometimes be deleterious to the nucleic acid complexation -the benefit to the overall transfection efficiency being affected by the casedependent relative importance of those two steps. [103][104][105] In contrast, although lysine-containing cationic polymers are better at nucleic acid complexation and cell penetration, 106,107 they do not display effective endosomolytic activity, thereby causing low transfection efficiency. 104,108…”

Hogyan befolyásolja a primer szerkezet elágazó láncú polipeptidek térszerkezetét és konjugátumaik funkcionális tulajdonságait?

“…Ugyanakkor a poli[L-Lys] toxikusnak bizonyult mind a HT-29 (LC 50 = 44.1 ± 4.6 ìg/mL), mind a HepG2 sejteken (LC 50 = 32.3 ± 10.8 ìg/mL). 40 Habár a citotoxikus/citosztatikus hatást nagyon különbözo sejteken vizsgáltunk, összefoglalásként érdemes felhívni a figyelmet arra, hogy az elágazó polipeptidek gerincét képezo poli[L-Lys] illetve az XAK (X= Lys) származék pusztító/gátló hatása jól megfigyelheto volt. Azok a polipeptidek, amelyek oldalláncaikban g-karboxil (pl.…”

Advanced Delivery Systems Based on Lysine or Lysine Polymers

“…They also studied internalization for different cell types and deduced that cell type is also important in the internalization process. 63 In most cases, it is necessary for the drug being delivered by polylysine to be released from the polymer. While this particular topic will be discussed in detail in the following sections, it is worth briefly considering here for the sake of completeness.…”

“…Polylysine with a positive charge group and containing hydrophobic amino acid close to the polylysine backbone can internalize efficiently. They also studied internalization for different cell types and deduced that cell type is also important in the internalization process …”

“…Chemical structure of branched polypeptides based on polylysine and their different side chains. Reprinted with permission . Copyright 2017 American Chemical Society.…”